.: ORİJİNAL ARAŞTIRMA
Diyabetik Ratlarda Aerobik Egzersizin Kortizol ve Tiroid Hormonları Üzerine Etkisi: Deneysel Araştırma
The Effect of Aerobic Exercise on Cortisol and Thyroid Hormones in Diabetic Rats: Experimental Research
Turkiye Klinikleri J Sports Sci. 2023;15(3):399-404
doi: 10.5336/sportsci.2023-96652
Makale Dili: TR
Tam Metin
ÖZET
Amaç: Bu araştırmada, deneysel diyabet oluşturulan ratlarda aerobik egzersizin kortizol ve tiroid hormonları üzerine etkilerinin belirlenmesi amaçlanmıştır. Gereç ve Yöntemler: Araştırmada, 6 haftalık, 36 adet Wistar Albino rat kullanıldı. Hayvanlar canlı ağırlıkları birbirine benzer olacak şekilde Kontrol (K), Diyabet (D), Egzersiz (E) ve Diyabet+Egzersiz (DE) olmak üzere 4 gruba ayrıldı. D ve DE gruplarına tek doz 60 mg/kg streptozotosin solüsyonu intraperitoneal olarak enjekte edildi. E ve DE gruplarına 4 hafta, 20 m/dk hızda ve günlük 45 dk koşu bandında egzersiz yaptırıldı. Deneme sonunda elde edilen serumlar ticari kit (Abbott) protokolüne uygun olarak belirlendi. Bulgular: Deneme sonunda elde edilen veriler incelendiğinde, D ve DE gruplarının glukoz değerlerinin kontrol gruplarına (K ve E) göre anlamlı düzeyde yüksek olduğu (p<0,05), ancak DE grubundaki değerlerin D grubuna göre azaldığı tespit edildi. D grubunda tiroid stimüle edici hormon [thyroid stimulating hormone (TSH)], T3 ve T4 seviyeleri K ve E grubuna kıyasla düşüş gösterdi (p<0,05). Kortizol düzeyi ise E grubunda diğer 3 gruptan daha düşük iken, en yüksek kortizol düzeyi diyabetik grupta ölçüldü (p<0,05). DE grubunda serum TSH düzeylerinin D grubuna kıyasla önemli oranda arttığı ve kontrol gruplarına (K ve E) benzerlik gösterdiği belirlendi (p<0,05). Yine DE grubunda T3, T4 düzeylerinin D grubuna göre artma eğiliminde olduğu belirlendi. Sonuç: Diyabetiklerde 4 hafta süren aerobik egzersiz uygulamasının, hem glukoz metabolizmasını iyileştirmesinde hem de kortizol ve tiroid hormon seviyelerini artırmasında oldukça etkili olduğu görülmektedir.
Anahtar Kelimeler: Aerobik egzersiz; diabetes mellitus; glukoz; kortizol; tiroid hormonları
Amaç: Bu araştırmada, deneysel diyabet oluşturulan ratlarda aerobik egzersizin kortizol ve tiroid hormonları üzerine etkilerinin belirlenmesi amaçlanmıştır. Gereç ve Yöntemler: Araştırmada, 6 haftalık, 36 adet Wistar Albino rat kullanıldı. Hayvanlar canlı ağırlıkları birbirine benzer olacak şekilde Kontrol (K), Diyabet (D), Egzersiz (E) ve Diyabet+Egzersiz (DE) olmak üzere 4 gruba ayrıldı. D ve DE gruplarına tek doz 60 mg/kg streptozotosin solüsyonu intraperitoneal olarak enjekte edildi. E ve DE gruplarına 4 hafta, 20 m/dk hızda ve günlük 45 dk koşu bandında egzersiz yaptırıldı. Deneme sonunda elde edilen serumlar ticari kit (Abbott) protokolüne uygun olarak belirlendi. Bulgular: Deneme sonunda elde edilen veriler incelendiğinde, D ve DE gruplarının glukoz değerlerinin kontrol gruplarına (K ve E) göre anlamlı düzeyde yüksek olduğu (p<0,05), ancak DE grubundaki değerlerin D grubuna göre azaldığı tespit edildi. D grubunda tiroid stimüle edici hormon [thyroid stimulating hormone (TSH)], T3 ve T4 seviyeleri K ve E grubuna kıyasla düşüş gösterdi (p<0,05). Kortizol düzeyi ise E grubunda diğer 3 gruptan daha düşük iken, en yüksek kortizol düzeyi diyabetik grupta ölçüldü (p<0,05). DE grubunda serum TSH düzeylerinin D grubuna kıyasla önemli oranda arttığı ve kontrol gruplarına (K ve E) benzerlik gösterdiği belirlendi (p<0,05). Yine DE grubunda T3, T4 düzeylerinin D grubuna göre artma eğiliminde olduğu belirlendi. Sonuç: Diyabetiklerde 4 hafta süren aerobik egzersiz uygulamasının, hem glukoz metabolizmasını iyileştirmesinde hem de kortizol ve tiroid hormon seviyelerini artırmasında oldukça etkili olduğu görülmektedir.
Anahtar Kelimeler: Aerobik egzersiz; diabetes mellitus; glukoz; kortizol; tiroid hormonları
ABSTRACT
Objective: In this study, it was aimed to determine the effects of aerobic exercise on cortisol and thyroid hormones in rats with experimental diabetes. Material and Methods: In the study, 36 Wistar Albino rats, 6 weeks old, were used. The animals were divided into 4 groups as Control (C), Diabetes (D), Exercise (E) and Diabetes+Exercise (DE) with similar body weights. A single dose of 60 mg/kg streptozotocin solution was injected intraperitoneally into groups D and DE. E and DE groups exercised on a treadmill for 4 weeks at a speed of 20 m/min and for 45 minutes per day. The sera obtained at the end of the experiment were determined in accordance with the commercial kit (Abbott) protocol. Results: When the data obtained at the end of the experiment were examined, it was determined that the glucose values of the D and DE groups were significantly higher than the control groups (C and E) (p<0.05), but the values in the DE group were decreased compared to the D group. Thyroid stimulating hormone (TSH), T3 and T4 levels in D group decreased compared to C and E groups (p<0.05). Cortisol levels between groups were the lowest in group E and highest in group D. It was determined that serum TSH levels in the DE group increased significantly compared to the D group and showed similarity to the control groups (C and E) (p<0.05). Again, it was determined that T3, T4 levels in the DE group tended to increase compared to the D group. Conclusion: It is seen that aerobic exercise lasting 4 weeks in diabetics is very effective in both improving glucose metabolism and increasing cortisol and thyroid hormone levels.
Keywords: Aerobic exercise; diabetes mellitus; glucose; cortisol; thyroid hormones
Objective: In this study, it was aimed to determine the effects of aerobic exercise on cortisol and thyroid hormones in rats with experimental diabetes. Material and Methods: In the study, 36 Wistar Albino rats, 6 weeks old, were used. The animals were divided into 4 groups as Control (C), Diabetes (D), Exercise (E) and Diabetes+Exercise (DE) with similar body weights. A single dose of 60 mg/kg streptozotocin solution was injected intraperitoneally into groups D and DE. E and DE groups exercised on a treadmill for 4 weeks at a speed of 20 m/min and for 45 minutes per day. The sera obtained at the end of the experiment were determined in accordance with the commercial kit (Abbott) protocol. Results: When the data obtained at the end of the experiment were examined, it was determined that the glucose values of the D and DE groups were significantly higher than the control groups (C and E) (p<0.05), but the values in the DE group were decreased compared to the D group. Thyroid stimulating hormone (TSH), T3 and T4 levels in D group decreased compared to C and E groups (p<0.05). Cortisol levels between groups were the lowest in group E and highest in group D. It was determined that serum TSH levels in the DE group increased significantly compared to the D group and showed similarity to the control groups (C and E) (p<0.05). Again, it was determined that T3, T4 levels in the DE group tended to increase compared to the D group. Conclusion: It is seen that aerobic exercise lasting 4 weeks in diabetics is very effective in both improving glucose metabolism and increasing cortisol and thyroid hormone levels.
Keywords: Aerobic exercise; diabetes mellitus; glucose; cortisol; thyroid hormones
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