Turkiye Klinikleri Journal of Dermatology

.: ORIGINAL RESEARCH
Psöriyazis Hastalarında Serum Endotelin-1 ve Nitrik Oksit Düzeylerinin Hastalığın Şiddeti ile İlişkisi: Prospektif Vaka-Kontrol Çalışması
The Relationship Between Serum Endothelin-1 and Nitric Oxide Levels and Severity of Psoriasis: A Prospective Case-Control Study
Bülent Nuri KALAYCIa, Gürsoy DOĞANb
aMalatya Turgut Özal Üniversitesi Tıp Fakültesi, Dermatoloji ABD, Malatya, Türkiye
bBaşkent Üniversitesi İzmir Zübeyde Hanım Uygulama ve Araştırma Merkezi, Deri ve Zührevi Hastalıkları ABD, İzmir, Türkiye
Turkiye Klinikleri J Dermatol. 2022;32(3):145-50
doi: 10.5336/dermato.2022-88501
Article Language: TR
Full Text
ÖZET
 Amaç: Psöriyazis, güçlü bir genetik yatkınlığı olan kronik, inflamatuar bir deri hastalığıdır. Serum endotelin-1 (ET-1) ve nitrik oksit (NO) düzeylerinin psöriyazis hastalarında arttığı gösterilmiştir. Çalışmamızda, serum ET-1 ve NO düzeyini psöriyazis hastalarında ve sağlıklı kontrollerde araştırmayı ve bu moleküller ile hastalık şiddeti arasındaki muhtemel ilişkiyi incelemeyi amaçladık. Gereç ve Yöntemler: Çalışmaya 34 psöriyazis hastası ile yaş ve cinsiyet uyumlu 35 sağlıklı kişi kontrol grubu olarak alındı. Hastalığın şiddeti, Psöriyazis Alan Şiddet İndeksi'ne (PASİ) göre analiz edildi. Hastalar, hastalığın şiddetine göre hafif, orta ve şiddetli olarak gruplandırıldı. Serum ET-1 düzeyleri ELİSA yöntemi kullanılarak belirlendi. Serum NO düzeyleri Griess reaktanı kullanılarak belirlendi. Bulgular: Hastaların yaş ortalaması 34,82±11 ve kontrol grubunun yaş ortalaması 31,86±8,5 yıl idi (p>0,05). Psöriyazis hastalarında ortalama serum ET-1 değeri 13,7±9,43 pg/mL, kontrol grubunda 9,90±3,05 pg/mL idi (p=0,02). Psöriyazis hastalarında ortalama serum nitrit (NO2) değeri 42,77±14,28 µmol/L, kontrol grubunda 37,53±10,72 µmol/L idi. Hasta ve kontrol grupları karşılaştırıldığında fark istatistiksel olarak anlamlı değildi (p>0,05). Psöriyazis hastalarında ortalama nitrat (NO3) değeri 39,04±14,68 µmol/L, kontrol grubunda 33,76±10,56 µmol/L idi. Hasta ve kontrol grupları karşılaştırıldığında fark istatistiksel olarak anlamlı değildi (p>0,05). Ortalama NO2 ve NO3 değerleri şiddetli grupta kontrol ve hafif gruba oranla istatistiksel olarak anlamlı derecede yüksekti (p=0,003, p=0,01). Hastaların serum NO2 ve NO3 değerleri ile PASİ skorları arasında, ET-1'e benzer şekilde bir korelasyon tespit edildi. Sonuç: Kronik plak tip psöriyaziste serum ET-1 ve NO düzeyleri artmış olup, hastalık şiddeti ile korelasyon mevcuttur. Hastalık şiddeti ile korelasyon göstermesi nedeniyle bu 2 parametre psöriyazis şiddetinin ölçülmesinde bir kriter olarak kullanılabilir.

Anahtar Kelimeler: Psöriyazis; endotelin-1; nitrik oksit
ABSTRACT
 Objective: Psoriasis is a chronic inflammatory skin disease that has a strong genetic background. Elevated serum levels of endothelin-1 (ET-1) and nitric oxide (NO) were observed in psoriasis patients. We aimed to explore serum ET-1 and NO in psoriasis patients and healthy controls and analyze the possible correlation between these molecules and disease severity. Material and Methods: The present study was conducted with 34 eligible psoriasis patients and 35 age- and gender-matched healthy controls. Disease severity was analyzed based on the Psoriasis Area Severity Index (PASI) scores. The patients were divided as mild, moderate and severe according to disease severity. Serum ET-1 levels were determined with ELISA method. NO levels were determined by using Griess reagent. Results: The mean age of patients were 34.82±11 and the mean age of controls were 31.86±8.5 years (p>0.05). The mean ET-1 level was 13.7±9.43 pg/mL in psoriasis and 9.90±3.05 pg/mL in control group (p=0.02). The mean nitrite (NO2) levels were 42.77±14.28 µmol/L in psoriasis and 37.53±10.72 µmol/L in control group (p>0.05). The mean nitrate (NO3) levels were 39.04±14.68 µmol/L in psoriasis and 33.76±10.56 µmol/L in control group. There was also no statistically significant difference between them (p>0.05). The mean NO2 and NO3 values were statistically significantly higher in the severe group compared to the control and mild groups (p=0.003, p=0.01). Correlations were found between serum NO levels and PASI scores of psoriasis as in ET-1 levels. Conclusion: Serum ET-1 and NO levels were found as high in psoriasis. We found corelations between ET-1, NO levels and PASI scores. Therefore these 2 parameters may be used as indicators of disease severity.

Keywords: Psoriasis; endothelin-1; nitric oxide
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