Turkiye Klinikleri Journal of Medical Sciences

.: ORIGINAL RESEARCH
Investigation of RN7SL1 Expression in Human Umbilical Vein Endothelial Cells After Oxidized Low-Density Lipoprotein Stimulation: Experimental Study
Oksitlenmiş Düşük-Yoğunluklu Lipoprotein Uyarımından Sonra İnsan Göbek Kordonu Damar Endotel Hücrelerinde RN7SL1 Ekspresyonunun Araştırılması: Deneysel Çalışma
Burcu BAYYURTa , Serdal ARSLANb
aSivas Cumhuriyet University Faculty of Medicine, Department of Medical Biology, Sivas, Türkiye
bMersin University Faculty of Medicine, Department of Medical Biology, Mersin, Türkiye
Turkiye Klinikleri J Med Sci. 2025;45(1):43-8
doi: 10.5336/medsci.2024-105015
Article Language: EN
Full Text
ABSTRACT
 Objective: Oxidized low-density lipoprotein (ox-LDL) damages endothelial cells (ECs) and induces pathogenic processes related to atherosclerosis (AS). Long non-coding RNAs (lncRNAs) have been accepted to be transcripts code no proteins and have important roles in gene expression regulation. LncRNAs have been thought important targets in the fight against cardiovascular diseases (CVDs). RNA component of signal recognition particle (SRP) 7SL1 (RN7SL1) is a lncRNA. RN7SL1 was associated with inhibition of cell cycle arrest. Since RN7SL1 is associated with cell cycle arrest and ox-LDL affects EC cell apoptosis, hypothesis of our study is that ox-LDL may alter the expression of this lncRNA in ECs. There was no study yet on RN7SL1 and CVDs including AS. We aimed to compare RN7SL1 gene expression between ECs treated with ox-LDL and normal ECs. Material and Methods: We investigated expression level of lncRNA RN7SL1 in human umbilical vein endothelial cells (HUVECs) induced with ox-LDL in this study. We performed measurement with SYBR green dye using quantitative PCR (real-time PCR, qPCR) method. Results: We found that expression of RN7SL1 was up-regulated 6.06 fold statistic significantly in HUVECs after ox-LDL treatment (P<0.001**). RN7SL1 upregulation may modulate EC cycle and apoptosis in AS, since ox-LDL stimulation increased expression of RN7SL1 gene in HUVECs. Conclusion: According to findings of the current study, our interest focused on that RN7SL1 may associated with vascular damage of the ECs at initation of the AS. Our results further will guide future studies related to the RN7SL1 and CVDs including AS.

Keywords: Gene expression; human umbilical vein endothelial cell; oxidized low-density lipoprotein; RN7SL1
ÖZET
 Amaç: Oksitlenmiş düşük-yoğunluklu lipoprotein [oxidized lowdensity lipoprotein (ox-LDL)] endotel hücrelerine [endothelial cells (ECs)] zarar verir ve ateroskleroz (AS) ile ilişkili patojenik süreçleri başlatır. Uzun kodlama yapmayan ribonükleik asitler (lncRNA'lar) protein kodlamayan transkriptler olarak kabul edilir ve gen ifadesinin düzenlenmesinde önemli rolleri vardır. LncRNA'ların kardiyovasküler hastalıklarla [cardiovascular diseases (CVD)] mücadelede önemli hedefler olduğu düşünülmektedir. Sinyal tanıma partikülünün [signal recognition particle (SRP)] RNA bileşeni 7SL1 (RN7SL1) bir lncRNA'dır. RN7SL1, hücre döngüsü durmasının inhibisyonu ile ilişkilendirilmiştir. RN7SL1; hücre döngüsünün durdurulması ile ilişkili olduğundan ve ox-LDL; EC hücresi apoptozunu etkilediğinden, çalışmamızın hipotezi, ox-LDL'nin, bu lncRNA'nın EC'lerdeki ekspresyonunu değiştirebileceği yönündedir. RN7SL1 ve CVD'ler kapsamındaki AS ile ilişkili henüz bir çalışma yapılmamıştır. Çalışmamızda ox-LDL ile muamele edilen ve normal EC'ler arasında RN7SL1 gen ekspresyonunu karşılaştırmayı amaçladık. Gereç ve Yöntemler: Bu çalışmada ox-LDL ile indüklenen insan göbek kordonu damar endotel hücrelerinde [human umbilical vein endothelial cells (HUVEC'ler)] lncRNA RN7SL1 ekspresyon düzeyini araştırdık. Kantitatif polimeraz zincir reaksiyonu [polymerase chain reaction (PCR)] (gerçek zamanlı PCR, qPCR) yöntemini kullanarak SYBR green boyası ile ölçüm yaptık. Bulgular: Ox-LDL uyarımından sonra HUVEC'lerde RN7SL1 ekspresyonunun istatistiksel olarak anlamlı şekilde 6,06 kat arttığını bulduk (p<0,001**). Ox-LDL uyarımı HUVEC'lerde RN7SL1 geninin ekspresyonunu artırdığından, RN7SL1'in yukarı regülasyonu AS'de EC döngüsünü ve apoptozu düzenleyebilir. Sonuç: Mevcut çalışmanın bulgularına göre, RN7SL1'in AS başlangıcında EC'lerin vasküler hasarı ile ilişkili olabileceği düşünülmektedir. Sonuçlarımız ayrıca RN7SL1 ve AS dâhil CVD'lerle ilgili gelecekteki çalışmalara rehberlik edecektir.

Anahtar Kelimeler: Gen ekspresyonu; insan göbek kordonu damar endotel hücre; oksitlenmiş düşük-yoğunluklu lipoprotein; RN7SL1
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