Turkiye Klinikleri Cardiovascular Sciences

.: ORIGINAL RESEARCH
An Investigation of the Effects of Duloxetine on the Heart
Duloksetinin Kalp Üzerindeki Etkilerinin Araştırılması
Işıl ERANILa, Nigar VARDIa, Azibe YILDIZa, Hakan PARLAKPINARb, Onural ÖZHANb
aDepartment of Histology and Embryology, İnönü University Faculty of Medicine, Malatya, TURKEY
bDepartment of Medical Pharmacology, İnönü University Faculty of Medicine, Malatya, TURKEY
Turkiye Klinikleri J Cardiovasc Sci. 2020;32(3):116-29
doi: 10.5336/cardiosci.2020-74496
Article Language: EN
Full Text
ABSTRACT
 Objevtive: The present study was designed to examine the effects of high dose and low dose duloxetine on Cx43, a gap junction (GJ) protein; on S100A, cardiac contractility component and on certain early cardiac impairment parameters as TnI and TnT. Material and Methods: The study was performed with 24 male Wistar Albino rats by generating control group (1 ml solvent), low-dose duloxetine (LDD) group (10 mg/kg) and high-dose duloxetine (HDD) group (100 mg/kg). After the experimental procedure, the results concerning blood pressure and heart rates of the anesthetized rats were recorded and HE staining and immunostaining of Cx43, S100A, TnI and TnT were applied to the heart tissue sections. Results: In the control group, Cx43 was stained as marked streaks between cardiomyocytes; TnI, TnT and S100A were stained as homogenous and dark brown in cytoplasm. Nevertheless, intensity of Cx43 immunostaining showed significant increase in duloxetine- treated groups (p0.05). Conclusion: Our study reveals that duloxetine induced an increase in the immunostaining of Cx43, and a decrease in the immunostaining of TnI, TnT and S100A, also known as early cardiac parameters. Furthermore, duloxetine was observed to show no effect on oxidant and antioxidant parameters. While heart rate remained unchanged, mean blood pressure decreased in high dose duloxetine group. In the light of the study outcomes, it has been concluded that antidepressants must be administered more advertently in depression patients with heart disorders.

Keywords: Rat; heart; antidepressant; duloxetine; immunohistochemistry
ÖZET
 Amaç: Bu çalışmada düşük ve yüksek doz duloksetinin, gap junction bileşenlerinden Cx43, kardiyak kontraktilite komponenti S100A ve kardiyak kasılma belirteci olarak kullanılan TnI ve TnT gibi erken kardiyak hasar parametreleri üzerindeki etkilerinin değerlendirilmesi amaçlanmaktadır. Gereç ve Yöntemler: Wistar Albino cinsi 24 adet erkek sıçan; kontrol (1 ml çözücü), düşük doz duloksetin (DDD) (10 mg/kg) ve yüksek doz duloksetin (YDD) (100 mg/kg) şeklinde üç gruba ayrıldı. Deney protokolü bitiminde anestezi altındaki sıçanların kan basıncı ile kalp hızı sonuçları kaydedildi ve elde edilen doku kesitlerine H-E boyama yöntemi, Cx43, S100A, TnI ve TnT immün boyama yöntemleri uygulandı. Bulgular: Kontrol grubunda Cx43 kardiyomiyositlerin bağlantı bölgelerinde belirgin çizgilenmeler şeklinde, TnI, TnT ve S100A ise sitoplazma içerisinde homojen ve koyu kahverengi olarak boyandı. Diğer yandan, duloksetin uygulanan gruplarda Cx43 immünreaktivite şiddetinde artış gözlendi (p0,05). Sonuç: Çalışmamız duloksetinin erken kardiyak hasar parametreleri olarak bilinen Cx43 immünoreaktivesinde artışa, TnI, TnT ve S100A'da ise azalmaya neden olduğunu gösterdi. Ayrıca oksidan- antioksidan parametreler ile kalp hızı üzerine herhangi bir değişikliğe neden olmadığı, ancak doza bağlı olarak ortalama kan basıncını azalttığı gözlendi. Bütün bunlar göz önüne alınarak, kalp rahatsızlığı olan depresyon hastalarında, antidepresanların da daha dikkatli uygulanması gerektiği sonucuna varıldı.

Anahtar Kelimeler: Sıçan; kalp; antidepresan; duloksetin; immünohistokimya
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www.turkiyeklinikleri.com uses the taken information:

-To enhance, improve and maintain the quality of the website

-To generate visitor’s profile and statistical data

-To determine the tendency of the visitors on using our website

-To send print publications/correspondences

-To send press releases or notifications through e-mail

-To generate a list for an event or competition

By using www.turkiyeklinikleri.com you are considered to agree that;

-Ortadoğu Advertisement Presentation Publishing Tourism Education Architecture Industry and Trade Inc. cannot be hold responsible for any user’s illegal and immoral behavior,

-Terms of use may change from time to time,

-It is not responsible for other websites’ contents it cannot control or the harms they may cause although it uses the connection they provided.

Ortadoğu Advertisement Presentation Publishing Tourism Education Architecture Industry and Trade Inc. may block the website to users in the following events:

-Information with wrong, incomplete, deceiving or immoral expressions is recorded to the website,

-Proclamation, advertisement, announcement, libelous expressions are used against natural person or legal identity,

-During various attacks to the website,

-Disruption of the website because of a virus.

Written, visual and audible materials of the website, including the code and the software are under protection by legal legislation.

Without the written consent of Ortadoğu Advertisement Presentation Publishing Tourism Education Architecture Industry and Trade Inc. the information on the website cannot be downloaded, changed, reproduced, copied, republished, posted or distributed.

All rights of the software and the design of the website belong to Ortadoğu Advertisement Presentation Publishing Tourism Education Architecture Industry and Trade Inc.

Ortadoğu Advertisement Presentation Publishing Tourism Education Architecture Industry and Trade Inc. will be pleased to hear your comments about our terms of use. Please share the subjects you think may enrich our website or if there is any problem regarding our website.

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