Turkiye Klinikleri Journal of Medical Sciences

.: ORIGINAL RESEARCH
An Experimental Study on Investigation of Myokine Responses to Acute and Chronic Swimming Exercise in Mice
Farelerde Akut ve Kronik Yüzme Egzersizine Miyokin Cevaplarının İncelenmesi Üzerine Deneysel Bir Çalışma
Egem Burcu ÜNALa , Özgen KILIÇ ERKEKb , Melek BOR KÜÇÜKATAYb
aAdana City Training and Research Hospital, Clinic of Physiology, Adana, Türkiye
bPamukkale University Faculty of Medicine, Department of Physiology, Denizli, Türkiye
Turkiye Klinikleri J Med Sci. 2024;44(2):60-8
doi: 10.5336/medsci.2023-100684
Article Language: EN
Full Text
ABSTRACT
 Objective: Exercise is known to have many beneficial effects for the organism, especially the musculoskeletal, cardiovascular, respiratory and endocrine systems, depending on the type, intensity, duration and frequency. One of the mechanisms that mediate these beneficial effects is cytokines released from muscle called myokines. It is important to examine myokine levels in response to exercise to understand their roles in muscle growth / differentiation, metabolism, angiogenesis, neuronal stimulation. We aimed to investigate the time-dependent changes in the plasma levels of ciliary neurotrophic factor (CNTF), CXC ligand 1 (CXCL-1), cardiotrophin 1 (CT-1), oncostatin M (OSM), myostatin (MSTN) in response to acute-chronic swimming exercise in mice. Material and Methods: 70 adult, BALB/c male mice were divided into control, acute swimming (30 min) and chronic swimming (30 min/day, 5 days/week, 6 weeks) groups. Exercising mice were further divided into 3, in terms of the time passed (3, 24, 48 h) following the last exercise session. Mice swam in a water tank (68 cm x 44 cm x 38 cm) at constant temperature (30-32 °C). Plasma CNTF, CXCL-1, CT-1, OSM, MSTN levels were determined using commercial kits. Results: No statistically significant time-dependent alteration in plasma levels of CNTF, CXCL1, CT-1, OSM, MSTN was determined. Conclusion: The fact that there is no change in plasma myokine concentrations at 3, 24 and 48 h following exercises may not exclude the possibility that these myokines play role in exercise-induced adaptations. If different sample collection times had been chosen, possible changes in these myokines may have been detected.

Keywords: Adaptation; myokine; swimming
ÖZET
 Amaç: Egzersizin; tipine, şiddetine, süresine ve sıklığına bağlı olarak başta kas-iskelet sistemi, kardiyovasküler sistem, solunum sistemi, endokrin sistem olmak üzere organizma için çok sayıda yararlı etkileri olduğu bilinmektedir. Egzersizin bu faydalı etkilerine aracılık eden mekanizmalardan birisi de miyokin adı verilen kastan salınan sitokinlerdir. Miyokinlerin kas büyüme / farklılaşması, metabolizması, yeni damar oluşumu ve kasın sinirsel uyarımındaki rollerini anlamak için egzersize cevaben düzeylerinin incelenmesi önem kazanmaktadır. Bu çalışmada, farelerde akut-kronik yüzme egzersizine yanıt olarak siliyer nörotrofik faktör [ciliary neurotrophic factor (CNTF)], CXC ligand 1 (CXCL-1), kardiyotrofin 1 [cardiotrophin 1 (CT-1)], onkostatin M (OSM), miyostatin (MSTN) miyokinlerinin plazma düzeylerindeki zamana bağlı değişimlerin araştırılması amaçlandı. Gereç ve Yöntemler: 70 adet yetişkin, BALB/c erkek fare; kontrol, akut yüzme (30 dk) ve kronik yüzme (30 dk/gün, 5 gün/hafta, 6 hafta) gruplarına ayrıldı. Egzersiz yapan fareler, son egzersiz seansından sonra geçen süreye göre (3, 24, 48 saat) ayrıca 3'e bölündü. Fareler, su tankında (68 cm x 44 cm x 38 cm), sabit sıcaklıkta (30-32 °C) yüzdürüldü. Plazma CNTF, CXCL-1, CT-1, OSM ve MSTN düzeyleri ticari kitler kullanılarak ölçüldü. Bulgular: CNTF, CXCL-1, CT-1, OSM, MSTN'nin plazma düzeylerinde zamana bağlı istatistiksel olarak anlamlı bir değişiklik saptanmadı. Sonuç: Egzersiz sonrası 3, 24 ve 48. saatlerde plazma miyokin konsantrasyonlarında herhangi bir değişiklik olmaması, bu miyokinlerin egzersize bağlı adaptasyonlarda rol oynama olasılığını dışlamayabilir. Farklı numune toplama zamanları seçilseydi bu miyokinlerdeki olası değişiklikler tespit edilebilirdi.

Anahtar Kelimeler: Adaptasyon; miyokin; yüzme
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