.: ORİJİNAL ARAŞTIRMA
Vitiligolu Çocuklarda Total Oksidan ve Antioksidan Kapasite: Bir Olgu-Kontrol Çalışması
Total Oxidant and Total Antioxidant Capacity in Children with Vitiligo: A Case-Control Study
Turkiye Klinikleri J Dermatol. 2022;32(3):170-4
doi: 10.5336/dermato.2022-91939
Makale Dili: TR
Tam Metin
ÖZET
Amaç: Vitiligo; etiyolojisi tam olarak açıklanamayan, melanosit destrüksiyonuyla karakterize, klinik olarak iyi sınırlanmış depigmente lezyonlarla kendini gösteren herediter, kazanılmış bir hastalık olarak bilinmektedir. Bu çalışmada, vitiligolu çocuklarda total oksidan kapasite [total oxidant capacity (TOS)], total antioksidan kapasite [total antioxidant capacity (TAS)] düzeyleri ve oksidatif stres indeksinin (OSI) hesaplanması amaçlandı. Gereç ve Yöntemler: Çalışma grubunda; TOS ve TAS düzeyleri, kolorimetrik yöntem ile Rel Assay kitleri kullanılarak çalışıldı. OSI; (TOS, umol H202 equivalent/L/TAS, umol Trolox equivalent/L)x100 formülü ile hesaplandı. Çalışma grubu 30 (13 erkek, 17 kız) vitiligolu hasta ve 30 (16 erkek, 14 kız) sağlıklı kontrol grubundan oluşmaktaydı (p>0,05). Bulgular: Vitiligolu çocukların yaş ortalaması 9,17±4,87 iken, vitiligosu olmayan sağlıklı çocukların yaş ortalaması 9,27± 3,88 idi. Vitiligolu hastalarda ve sağlıklı kontrol grubunda TAS düzeyleri sırasıyla 0,996±0,124 ve 1,032±0,097 umol Trolox equivalent/L olarak bulundu (p>0,05). TOS düzeyleri ise vitiligolu hastalarda 3,98±3,14 ve sağlıklı kontrol grubunda 3,61±1,43 umol H202 equivalent/L olarak bulundu (p>0,05). OSI değeri ise vitiligolu hastalarda 0,399±0,30 ve kontrol grubunda 0,351± 0,14 olarak hesaplandı (p>0,05). Sonuç: Sonuç olarak vitiligolu hasta grubu ile kontrol grubunda TAS, TOS ve OSI değerleri arasında anlamlı bir fark bulunmadı. Çalışmamızda, nonenzimatik serum TAS ölçüldü. Buna göre TAS düzeyi düşük, TOS ve OSI düzeyleri yüksek olmasına rağmen bu değerler istatistiksel olarak anlamlı değildi. Sonuç olarak vitiligolu çocuk hastalarda, organizmanın oksidatif strese girmediği düşünülebilir ve bu sonuçlar daha ileri çalışmalarla desteklenebilir.
Anahtar Kelimeler: Vitiligo; total oksidan kapasite; total antioksidan kapasite
Amaç: Vitiligo; etiyolojisi tam olarak açıklanamayan, melanosit destrüksiyonuyla karakterize, klinik olarak iyi sınırlanmış depigmente lezyonlarla kendini gösteren herediter, kazanılmış bir hastalık olarak bilinmektedir. Bu çalışmada, vitiligolu çocuklarda total oksidan kapasite [total oxidant capacity (TOS)], total antioksidan kapasite [total antioxidant capacity (TAS)] düzeyleri ve oksidatif stres indeksinin (OSI) hesaplanması amaçlandı. Gereç ve Yöntemler: Çalışma grubunda; TOS ve TAS düzeyleri, kolorimetrik yöntem ile Rel Assay kitleri kullanılarak çalışıldı. OSI; (TOS, umol H202 equivalent/L/TAS, umol Trolox equivalent/L)x100 formülü ile hesaplandı. Çalışma grubu 30 (13 erkek, 17 kız) vitiligolu hasta ve 30 (16 erkek, 14 kız) sağlıklı kontrol grubundan oluşmaktaydı (p>0,05). Bulgular: Vitiligolu çocukların yaş ortalaması 9,17±4,87 iken, vitiligosu olmayan sağlıklı çocukların yaş ortalaması 9,27± 3,88 idi. Vitiligolu hastalarda ve sağlıklı kontrol grubunda TAS düzeyleri sırasıyla 0,996±0,124 ve 1,032±0,097 umol Trolox equivalent/L olarak bulundu (p>0,05). TOS düzeyleri ise vitiligolu hastalarda 3,98±3,14 ve sağlıklı kontrol grubunda 3,61±1,43 umol H202 equivalent/L olarak bulundu (p>0,05). OSI değeri ise vitiligolu hastalarda 0,399±0,30 ve kontrol grubunda 0,351± 0,14 olarak hesaplandı (p>0,05). Sonuç: Sonuç olarak vitiligolu hasta grubu ile kontrol grubunda TAS, TOS ve OSI değerleri arasında anlamlı bir fark bulunmadı. Çalışmamızda, nonenzimatik serum TAS ölçüldü. Buna göre TAS düzeyi düşük, TOS ve OSI düzeyleri yüksek olmasına rağmen bu değerler istatistiksel olarak anlamlı değildi. Sonuç olarak vitiligolu çocuk hastalarda, organizmanın oksidatif strese girmediği düşünülebilir ve bu sonuçlar daha ileri çalışmalarla desteklenebilir.
Anahtar Kelimeler: Vitiligo; total oksidan kapasite; total antioksidan kapasite
ABSTRACT
Objective: Vitiligo; It is known as a hereditary, acquired disease of unexplained etiology, characterized by melanocyte destruction and clinically manifested by well-defined depigmented lesions. In this study, we aimed to compare the total oxidant capacity (TOS) and total antioxidant capacity (TAS) levels and oxidative stress indices (OSI) of children with vitiligo and healthy control group. Material and Methods: In the study group; TOS and TAS levels were studied in the Modular P800 autoanalyser by colorimetric method using Rel Assay kits. OSI was calculated by (TOS, umol H202 equivalent/L/TAS, umol Trolox equivalent/L)x100 formula. The study group consisted of 30 vitiligo patients (13 boys, 17 girls) with vitiligo and 30 healthy control group (16 boys, 14 girls) (p>0.05). Results: While the mean age of the children with vitiligo 9.17± 4.87, the mean age of the healthy children without vitiligo was 9.27± 3.88. TAS levels in vitiligo patients and the healthy control group were found 0.996±0.124 and 1.032±0.097 µmol Trolox equivalent/L respectively (p>0.05). TOS levels were found 3.98±3.14 in vitiligo patients and 3.61±1.43 µmol H2O2 equivalent/L in the healthy control group (p>0.05). OSI value was calculated as 0.399±0.30 in patients with vitiligo and 0.351±0.14 in the control group (p>0.05). Conclusion: As a result, no significant difference was found between TAS, TOS, and OSI values in the vitiligo patients group and the control group. In our study, serum nonenzymatic TAS was measured. Accordingly, we found low TAS levels and higher TOS and OSI levels in children with vitiligo than the control group, but these values were not statistically significant. We believe that the organism does not undergo oxidative stress in children with vitiligo and these results should be supported by further studies.
Keywords: Vitiligo; total oxidant capacity; total antioxidant capacity
Objective: Vitiligo; It is known as a hereditary, acquired disease of unexplained etiology, characterized by melanocyte destruction and clinically manifested by well-defined depigmented lesions. In this study, we aimed to compare the total oxidant capacity (TOS) and total antioxidant capacity (TAS) levels and oxidative stress indices (OSI) of children with vitiligo and healthy control group. Material and Methods: In the study group; TOS and TAS levels were studied in the Modular P800 autoanalyser by colorimetric method using Rel Assay kits. OSI was calculated by (TOS, umol H202 equivalent/L/TAS, umol Trolox equivalent/L)x100 formula. The study group consisted of 30 vitiligo patients (13 boys, 17 girls) with vitiligo and 30 healthy control group (16 boys, 14 girls) (p>0.05). Results: While the mean age of the children with vitiligo 9.17± 4.87, the mean age of the healthy children without vitiligo was 9.27± 3.88. TAS levels in vitiligo patients and the healthy control group were found 0.996±0.124 and 1.032±0.097 µmol Trolox equivalent/L respectively (p>0.05). TOS levels were found 3.98±3.14 in vitiligo patients and 3.61±1.43 µmol H2O2 equivalent/L in the healthy control group (p>0.05). OSI value was calculated as 0.399±0.30 in patients with vitiligo and 0.351±0.14 in the control group (p>0.05). Conclusion: As a result, no significant difference was found between TAS, TOS, and OSI values in the vitiligo patients group and the control group. In our study, serum nonenzymatic TAS was measured. Accordingly, we found low TAS levels and higher TOS and OSI levels in children with vitiligo than the control group, but these values were not statistically significant. We believe that the organism does not undergo oxidative stress in children with vitiligo and these results should be supported by further studies.
Keywords: Vitiligo; total oxidant capacity; total antioxidant capacity
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