.: ORİJİNAL ARAŞTIRMA
Tekrarlayan Gebelik Kaybı ile İlişkili Apoptotik Ölüm Reseptörü-4 TRAIL Bağlanma Bölgesindeki C626G Polimorfizmi: Vaka-Kontrol Araştırması
C626G Polymorphism in the Apoptotic Death Receptor-4 TRAIL Binding Domain Associated with Recurrent Pregnancy Loss: Case-Control Research
Turkiye Klinikleri J Med Sci. 2021;41(4):478-84
doi: 10.5336/medsci.2021-83700
Makale Dili: TR
Tam Metin
ÖZET
Amaç: Embriyonik gelişim sürecinde hücrelerde apoptotik dengenin bozulması gebelik kayıplarına neden olabilmektedir. Bu araştırmada, nedeni belirlenemeyen tekrarlayan gebelik kaybı (TGK) ile apoptotik uyarıcı ölüm reseptörü-4 [death receptor-4 (DR-4)] geni polimorfizmleri arasındaki ilişki araştırıldı. Gereç ve Yöntemler: Bu araştırmada, TGK tanısı almış 70 kadın ve kontrol grubu olarak 70 kadından genomik DNA elde edildi. Her 2 gruptaki DR-4 geni 3. ekzonundaki rs6557634 G422A, 4. ekzonundaki rs20575 C626G ve 5. ekzonundaki rs20576 A683C polimorfizmleri polimeraz zincir reaksiyonu-kısıtlayıcı enzim kesimi fragman uzunluk polimorfizmi yöntemi kullanılarak belirlendi. Bulgular: DR-4'ün ekstrasellüler kısmında TRAIL ligand bağlanma bölgesindeki 209. kodonda treoninin arginine değişimi ile sonuçlanan ekzon 4'teki bir missense değişim olan C626G polimorfizminin görülme sıklığının, TGK hastalarında kontrol grubuna göre arttığı saptandı. Araştırılan ekzon 3 ve ekzon 5 polimorfizmleri için TGK ve kontrol grubu arasında istatistiksel fark gözlenmedi. Sonuç: TGK hastalarında saptadığımız DR-4 genindeki missense ekzon 4 polimorfizminin, embriyonik gelişim sürecinde embriyonun uterusa invazyonu ve immünolojik toleransındaki apoptotik dengenin bozulması yoluyla, gebelik kayıplarına neden olabileceğini düşünmekteyiz.
Anahtar Kelimeler: Apoptoz; tekrarlayan gebelik kaybı; TRAIL; ölüm reseptörü-4; polimorfizm
Amaç: Embriyonik gelişim sürecinde hücrelerde apoptotik dengenin bozulması gebelik kayıplarına neden olabilmektedir. Bu araştırmada, nedeni belirlenemeyen tekrarlayan gebelik kaybı (TGK) ile apoptotik uyarıcı ölüm reseptörü-4 [death receptor-4 (DR-4)] geni polimorfizmleri arasındaki ilişki araştırıldı. Gereç ve Yöntemler: Bu araştırmada, TGK tanısı almış 70 kadın ve kontrol grubu olarak 70 kadından genomik DNA elde edildi. Her 2 gruptaki DR-4 geni 3. ekzonundaki rs6557634 G422A, 4. ekzonundaki rs20575 C626G ve 5. ekzonundaki rs20576 A683C polimorfizmleri polimeraz zincir reaksiyonu-kısıtlayıcı enzim kesimi fragman uzunluk polimorfizmi yöntemi kullanılarak belirlendi. Bulgular: DR-4'ün ekstrasellüler kısmında TRAIL ligand bağlanma bölgesindeki 209. kodonda treoninin arginine değişimi ile sonuçlanan ekzon 4'teki bir missense değişim olan C626G polimorfizminin görülme sıklığının, TGK hastalarında kontrol grubuna göre arttığı saptandı. Araştırılan ekzon 3 ve ekzon 5 polimorfizmleri için TGK ve kontrol grubu arasında istatistiksel fark gözlenmedi. Sonuç: TGK hastalarında saptadığımız DR-4 genindeki missense ekzon 4 polimorfizminin, embriyonik gelişim sürecinde embriyonun uterusa invazyonu ve immünolojik toleransındaki apoptotik dengenin bozulması yoluyla, gebelik kayıplarına neden olabileceğini düşünmekteyiz.
Anahtar Kelimeler: Apoptoz; tekrarlayan gebelik kaybı; TRAIL; ölüm reseptörü-4; polimorfizm
ABSTRACT
Objective: Disruption of apoptotic balance in cells during embryonic development can lead to pregnancy losses. In this study, the relationship between unexplained recurrent pregnancy loss (RPL) and apoptotic inducing death receptor-4 (DR-4) gene polymorphisms was investigated. Material and Methods: In this study, genomic DNA was obtained from 70 women diagnosed with RPL and 70 women as a control group. The polymorphisms of rs6557634 G422A in the 3rd exon of the DR-4 gene, rs20575 C626G in the 4th exon and rs20576 A683C in the 5th exon were determined in both groups using the polyme rase chain reaction-restriction fragment length polymorphism method. Results: It was determined that the incidence of C626G polymorphism, which is a missense variant in exon 4 resulting in the change of threonine to arginine in the 209th codone in the extracellular TRAIL ligand binding domain of DR-4, increased in RPL cases compared to the controls. For the investigated exon 3 and exon 5 polymorphisms, no statistical difference was observed between the RPL and the control group. Conclusion: We conclude that the missense exon 4 polymorphism in the DR-4 gene, which we detected in patients with RPL, may cause pregnancy losses through the invasion of the embryo to the uterus during embryonic development and the disruption of the apoptotic balance in its immunological tolerance.
Keywords: Apoptosis; recurrent pregnancy loss; TRAIL; death receptor-4; polymorphism
Objective: Disruption of apoptotic balance in cells during embryonic development can lead to pregnancy losses. In this study, the relationship between unexplained recurrent pregnancy loss (RPL) and apoptotic inducing death receptor-4 (DR-4) gene polymorphisms was investigated. Material and Methods: In this study, genomic DNA was obtained from 70 women diagnosed with RPL and 70 women as a control group. The polymorphisms of rs6557634 G422A in the 3rd exon of the DR-4 gene, rs20575 C626G in the 4th exon and rs20576 A683C in the 5th exon were determined in both groups using the polyme rase chain reaction-restriction fragment length polymorphism method. Results: It was determined that the incidence of C626G polymorphism, which is a missense variant in exon 4 resulting in the change of threonine to arginine in the 209th codone in the extracellular TRAIL ligand binding domain of DR-4, increased in RPL cases compared to the controls. For the investigated exon 3 and exon 5 polymorphisms, no statistical difference was observed between the RPL and the control group. Conclusion: We conclude that the missense exon 4 polymorphism in the DR-4 gene, which we detected in patients with RPL, may cause pregnancy losses through the invasion of the embryo to the uterus during embryonic development and the disruption of the apoptotic balance in its immunological tolerance.
Keywords: Apoptosis; recurrent pregnancy loss; TRAIL; death receptor-4; polymorphism
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