.: ORİJİNAL ARAŞTIRMA
Protective Effect of Curcumin Against Pancreatic Islet Injury in Streptozocin Induced Type 2 Diabetic Rats: An Experimental Study
Tip 2 Diyabetik Sıçanlarda Kurkuminin Streptozosin Kaynaklı Pankreatik Adacık Hasarına Karşı Koruyucu Etkisi: Deneysel Bir Çalışma
J Tradit Complem Med. 2022;5(3):187-93
doi: 10.5336/jtracom.2022-89199
Makale Dili: EN
Tam Metin
ABSTRACT
Objective: The aim of study of was to determine the damage caused by streptozocin (STZ)-induced Type 2 diabetes in pancreatic islet in rats and the effect of curcumin on this damage. Material and Methods: A total of 32 (250-320 g) Wistar albino male rats were used. Rats were randomly divided into 4 groups as Control (C), Diabetes (DM), Curcumin (CUR), and Diabetes+Curcumin (DM+CUR) (n=8). Histopathological, stereological and immunohistochemical analyzes were used to determine the damage caused by type 2 diabetes in the pancreatic tissue and the effect of curcumin on this damage. Results: In the histopathological evaluation of pancreatic tissues, in pancreatic sections of DM group rats, the islets were smaller and their borders were irregular, and degenerative and necrotic changes were observed in the cells. In the DM+CUR group, it was determined that these degenerative changes in the islets of Langerhans and cells were alleviated. In the stereological analysis, it was determined that the islet volume, the ratio of islets to the pancreas and the number of beta cells in the DM group were statistically significantly decreased compared to the other groups, and it was found to be higher in the DM+CUR group than in the DM group (p<0.05). In the immunohistochemical evaluation, the C and CUR groups showed similar staining with Ki67, whereas the staining was less in the diabetic group. In the diabetes group treated with curcumin, it was found that curcumin attenuated the diabetes- related Ki67 reduction. Conclusion: In this study, it was determined that curcumin reduced the damage in the islets and beta cells in the pancreas of rats with diabetes mellitus with STZ and provided a significant protection in the pancreatic tissue.
Keywords: Type 2 diabetes mellitus; curcumin; islets of Langerhans
Objective: The aim of study of was to determine the damage caused by streptozocin (STZ)-induced Type 2 diabetes in pancreatic islet in rats and the effect of curcumin on this damage. Material and Methods: A total of 32 (250-320 g) Wistar albino male rats were used. Rats were randomly divided into 4 groups as Control (C), Diabetes (DM), Curcumin (CUR), and Diabetes+Curcumin (DM+CUR) (n=8). Histopathological, stereological and immunohistochemical analyzes were used to determine the damage caused by type 2 diabetes in the pancreatic tissue and the effect of curcumin on this damage. Results: In the histopathological evaluation of pancreatic tissues, in pancreatic sections of DM group rats, the islets were smaller and their borders were irregular, and degenerative and necrotic changes were observed in the cells. In the DM+CUR group, it was determined that these degenerative changes in the islets of Langerhans and cells were alleviated. In the stereological analysis, it was determined that the islet volume, the ratio of islets to the pancreas and the number of beta cells in the DM group were statistically significantly decreased compared to the other groups, and it was found to be higher in the DM+CUR group than in the DM group (p<0.05). In the immunohistochemical evaluation, the C and CUR groups showed similar staining with Ki67, whereas the staining was less in the diabetic group. In the diabetes group treated with curcumin, it was found that curcumin attenuated the diabetes- related Ki67 reduction. Conclusion: In this study, it was determined that curcumin reduced the damage in the islets and beta cells in the pancreas of rats with diabetes mellitus with STZ and provided a significant protection in the pancreatic tissue.
Keywords: Type 2 diabetes mellitus; curcumin; islets of Langerhans
ÖZET
Amaç: Bu çalışmanın amacı, sıçanlarda streptozosin (STZ) ile indüklenmiş Tip 2 diyabetin, pankreas dokusunda oluşturduğu hasarı ve kurkuminin bu hasar üzerindeki etkisini belirlemektir. Gereç ve Yöntemler: Çalışmada toplamda 32 (250-320 g) Wistar albino erkek sıçan kullanıldı. Sıçanlar rastgele Kontrol (K), Diyabet (DM), Kurkumin (KUR) ve Diyabet+Kurkumin (DM+KUR) olmak üzere 4 gruba ayrıldı (n=8). Tip 2 diyabetin, pankreas dokusunda oluşturduğu hasar ve kurkuminin bu hasar üzerindeki koruyucu etkisini belirlemek için histopatolojik, stereolojik ve immünohistokimyasal analizler kullanıldı. Bulgular: Pankreas dokularında yapılan histopatolojik değerlendirmede, DM grubu sıçanlarının pankreatik kesitlerinde adacıkların küçüldüğü ve sınırlarının düzensiz olduğu, hücrelerde dejeneratif ve nekrotik değişiklikler olduğu görüldü. DM+CUR grubunda ise langerhans adacıklarında ve hücrelerde meydana gelen bu dejeneratif değişikliklerin hafiflediği belirlendi. Yapılan stereolojik analizlerde, DM grubundaki adacık hacminin, adacıkların pankreasa oranının ve beta hücrelerinin sayısının diğer tüm gruplara göre istatistiksel olarak anlamlı azaldığı, DM+CUR grubunda ise DM grubuna göre daha fazla olduğu tespit edildi (p<0,05). İmmunohistokimyasal değerlendirmede, K ve KUR grubunun Ki67 ile benzer boyanma gösterdiği, diyabetli grupta ise boyanmanın az olduğu gözlendi. Kurkumin ile tedavi edilen diyabet grubunda ise kurkuminin diyabetle ilişkili Ki67 azalmasını hafiflettiği tespit edildi. Sonuç: Bu çalışmada, STZ ile diyabet oluşturulmuş sıçanların pankreaslarındaki adacıklarda ve beta hücrelerinde meydana gelen hasarı kurkuminin indirgediği ve pankreas dokusunda önemli bir koruma sağladığı tespit edildi.
Anahtar Kelimeler: Tip 2 diabetes mellitus; kurkumin; Langerhans adacıkları
Amaç: Bu çalışmanın amacı, sıçanlarda streptozosin (STZ) ile indüklenmiş Tip 2 diyabetin, pankreas dokusunda oluşturduğu hasarı ve kurkuminin bu hasar üzerindeki etkisini belirlemektir. Gereç ve Yöntemler: Çalışmada toplamda 32 (250-320 g) Wistar albino erkek sıçan kullanıldı. Sıçanlar rastgele Kontrol (K), Diyabet (DM), Kurkumin (KUR) ve Diyabet+Kurkumin (DM+KUR) olmak üzere 4 gruba ayrıldı (n=8). Tip 2 diyabetin, pankreas dokusunda oluşturduğu hasar ve kurkuminin bu hasar üzerindeki koruyucu etkisini belirlemek için histopatolojik, stereolojik ve immünohistokimyasal analizler kullanıldı. Bulgular: Pankreas dokularında yapılan histopatolojik değerlendirmede, DM grubu sıçanlarının pankreatik kesitlerinde adacıkların küçüldüğü ve sınırlarının düzensiz olduğu, hücrelerde dejeneratif ve nekrotik değişiklikler olduğu görüldü. DM+CUR grubunda ise langerhans adacıklarında ve hücrelerde meydana gelen bu dejeneratif değişikliklerin hafiflediği belirlendi. Yapılan stereolojik analizlerde, DM grubundaki adacık hacminin, adacıkların pankreasa oranının ve beta hücrelerinin sayısının diğer tüm gruplara göre istatistiksel olarak anlamlı azaldığı, DM+CUR grubunda ise DM grubuna göre daha fazla olduğu tespit edildi (p<0,05). İmmunohistokimyasal değerlendirmede, K ve KUR grubunun Ki67 ile benzer boyanma gösterdiği, diyabetli grupta ise boyanmanın az olduğu gözlendi. Kurkumin ile tedavi edilen diyabet grubunda ise kurkuminin diyabetle ilişkili Ki67 azalmasını hafiflettiği tespit edildi. Sonuç: Bu çalışmada, STZ ile diyabet oluşturulmuş sıçanların pankreaslarındaki adacıklarda ve beta hücrelerinde meydana gelen hasarı kurkuminin indirgediği ve pankreas dokusunda önemli bir koruma sağladığı tespit edildi.
Anahtar Kelimeler: Tip 2 diabetes mellitus; kurkumin; Langerhans adacıkları
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