.: ORİJİNAL ARAŞTIRMA
Natural Thermal Water Consumption Could be Capable of Improving Gut Mycobiome in Dogs with Inflammatory Bowel Disease and Dermatological Manifestations: Randomized Clinical Study
Yangısal Bağırsak Hastalığı ve Dermatolojik Bulguları Olan Köpeklerde Doğal Termal Su Tüketimi Bağırsak Mikobiyomunu Geliştirebilir: Randomize Klinik Çalışma
Turkiye Klinikleri J Vet Sci. 2023;14(1):1-10
doi: 10.5336/vetsci.2022-91433
Makale Dili: EN
Tam Metin
ABSTRACT
Objective: Given the gut microbiome is a supreme immunological and metabolic organ, much data is recognized within the intestinal bacteria whereas the prevalence along with phylogenetic interpretation of fungal organisms participating within intestinal ecosystem have not explored convincingly among dogs with inflammatory bowel disease (IBD). In the present study, the present authors' for the first time a) described the fungal gut mycobiome, b) analyzed disbiosis network for fungal kigdom, c) detected the efficacy of natural thermal water (nTW) on gut mycobiome. Material and Methods: For these purpose, MIDOG All-in One Microbial Test targetting Next-Generation DNA Sequencing was used to determining of fungal microbiome prior to and thereafter nTW in a total of 13 dogs with IBD. Results: Aspergillus sp. (n=5), Acremonium hyalinulum (n=4), Acremonium fusidoides-hansfordii (n=4) were frequently isolated as fungal pathogens whereas case VII exhibited 5 different species (at most) and 4 other dogs presented solely 1 species. Interestingly a 6 week course of nTW consumption resulted with disapperance of fungal pathogens in 12 out of 13 cases. On behalf of repeated Next-Generation DNA Sequencing at 6th week analysis of all 12 cases wered deemed more diverse gut mycobiome. Conclusion: Obtained data preserved protective/restorative roles of nTW on gut mycobiome in dogs with IBD and dermatological manifestations ivolved at the present study.
Keywords: Alkaline water; dog; inflamatory bowel disease; gut microbiome
Objective: Given the gut microbiome is a supreme immunological and metabolic organ, much data is recognized within the intestinal bacteria whereas the prevalence along with phylogenetic interpretation of fungal organisms participating within intestinal ecosystem have not explored convincingly among dogs with inflammatory bowel disease (IBD). In the present study, the present authors' for the first time a) described the fungal gut mycobiome, b) analyzed disbiosis network for fungal kigdom, c) detected the efficacy of natural thermal water (nTW) on gut mycobiome. Material and Methods: For these purpose, MIDOG All-in One Microbial Test targetting Next-Generation DNA Sequencing was used to determining of fungal microbiome prior to and thereafter nTW in a total of 13 dogs with IBD. Results: Aspergillus sp. (n=5), Acremonium hyalinulum (n=4), Acremonium fusidoides-hansfordii (n=4) were frequently isolated as fungal pathogens whereas case VII exhibited 5 different species (at most) and 4 other dogs presented solely 1 species. Interestingly a 6 week course of nTW consumption resulted with disapperance of fungal pathogens in 12 out of 13 cases. On behalf of repeated Next-Generation DNA Sequencing at 6th week analysis of all 12 cases wered deemed more diverse gut mycobiome. Conclusion: Obtained data preserved protective/restorative roles of nTW on gut mycobiome in dogs with IBD and dermatological manifestations ivolved at the present study.
Keywords: Alkaline water; dog; inflamatory bowel disease; gut microbiome
ÖZET
Amaç: Bağırsak mikrobiyomunun üstün bir immünolojik ve metabolik organ olduğu göz önüne alındığında, intestinal bakteriler için çok fazla veri tanımlanırken, yangısal bağırsak hastalığı olan köpeklerde intestinal ekosisteme katılan mantar organizmalarının filogenetik değerlendirmesi ile birlikte prevalansı ikna edici bir şekilde araştırılmamıştır. Bu çalışma ile araştırmacılar ilk defa a) fungal bağırsak mikrobiyomunun tanımlamış, b) fungal faunadaki disbiyozis ağını tanımlamış, c) doğal termal suyun bağırsak mikrobiyatası üzerine etkisini belirlemiştir. Gereç ve Yöntemler: Bu amaçlar doğrultusunda yangısal bağırsak hastalığı olan 13 köpekte doğal termal su tüketimi öncesi ve sonrası fungal mikrobiyatanın belirlenmesi için DNA sekansının belirlenmesine dayanan MIDOG ALL-in One Mikrobiyal Test kullanılmıştır. Bulgular: Fungal patojen olarak sıklıkla Aspergillus sp. (n=5), Acremonium hyalinulum (n=4), Acremonium fusidoides-hansfordii (n=4) izole edilmişken, olgu VII 5 farklı tür (en fazla) ve diğer 4 köpekte sadece 1 tür tespit edilmiştir. İlginç olarak 6 haftalık termal su kullanımı sonrası 13 vakanın 12'sinde fungal patojenler kaybolmuştur. Sonuç: Yangısal bağırsak hastalığı ve dermatolojik bulguları olan köpeklerde doğal termal suyun bağırsak mikrobiyomu üzerindeki koruyucu/yenileyici rolü bu çalışmadan elde edilen verilerle ortaya konulmuştur.
Anahtar Kelimeler: Alkali su; köpek; yangısal bağırsak hastalığı; bağırsak mikrobiyomu
Amaç: Bağırsak mikrobiyomunun üstün bir immünolojik ve metabolik organ olduğu göz önüne alındığında, intestinal bakteriler için çok fazla veri tanımlanırken, yangısal bağırsak hastalığı olan köpeklerde intestinal ekosisteme katılan mantar organizmalarının filogenetik değerlendirmesi ile birlikte prevalansı ikna edici bir şekilde araştırılmamıştır. Bu çalışma ile araştırmacılar ilk defa a) fungal bağırsak mikrobiyomunun tanımlamış, b) fungal faunadaki disbiyozis ağını tanımlamış, c) doğal termal suyun bağırsak mikrobiyatası üzerine etkisini belirlemiştir. Gereç ve Yöntemler: Bu amaçlar doğrultusunda yangısal bağırsak hastalığı olan 13 köpekte doğal termal su tüketimi öncesi ve sonrası fungal mikrobiyatanın belirlenmesi için DNA sekansının belirlenmesine dayanan MIDOG ALL-in One Mikrobiyal Test kullanılmıştır. Bulgular: Fungal patojen olarak sıklıkla Aspergillus sp. (n=5), Acremonium hyalinulum (n=4), Acremonium fusidoides-hansfordii (n=4) izole edilmişken, olgu VII 5 farklı tür (en fazla) ve diğer 4 köpekte sadece 1 tür tespit edilmiştir. İlginç olarak 6 haftalık termal su kullanımı sonrası 13 vakanın 12'sinde fungal patojenler kaybolmuştur. Sonuç: Yangısal bağırsak hastalığı ve dermatolojik bulguları olan köpeklerde doğal termal suyun bağırsak mikrobiyomu üzerindeki koruyucu/yenileyici rolü bu çalışmadan elde edilen verilerle ortaya konulmuştur.
Anahtar Kelimeler: Alkali su; köpek; yangısal bağırsak hastalığı; bağırsak mikrobiyomu
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