.: ORİJİNAL ARAŞTIRMA
Kurkuminin Oral Yolla Yem Katkısı Olarak Kullanımının İshalli Buzağılarda Zonulin Seviyeleri ile Dışkı Kıvamı ve Hijyen Skoru Üzerine Etkinliğinin Araştırılması: Kohort Çalışması
Investigation of the Effectiveness of Oral Use of Curcumin as a Feed Additive on Zonulin Levels, Fecal Consistency and Hygiene Score in Calves with Diarrhea: Cohort Study
Turkiye Klinikleri J Vet Sci. 2025;16(1):1-7
doi: 10.5336/vetsci.2024-101599
Makale Dili: TR
Tam Metin
ÖZET
Amaç: Zerdeçal, Curcuma longa L.'den ekstrakte edildiği hâli ile antiinflamatuar, antibakteriyel, antiviral, antikanser, antioksidan ve koksidiyostatik aktivitelere sahiptir. Muhtemelen üzerinde en sıklıkla çalışılan, doğal bileşiklerden biridir. Geviş getiren hayvanlarda, geçirgen bağırsağa yönelik çok az çalışma mevcuttur. Bu araştırmada, ishalli buzağılarda geçirgen bağırsak (zonulin seviyeleri), dışkı kıvamı ile gizli temizlik (gT) skoru üzerine etkilerinin araştırılması özgün değere ve amaca yönelik çıkarımlardır. Gereç ve Yöntemler: Sahada aktif çalışan veteriner hekimleri bünyesinde barındıran, 4 farklı çiftlik ile irtibat kuruldu, temas noktasında tüm çiftlik çalışanlarından anamnez alındı. Bunun yani sıra gerek örneklem temininde, gerekse kan tahlillerinin alınmasında destek ve mukavemet tedariği mümkün kılındı. Her 4 çiftlikten toplamda n=19 buzağıda öncelikle veteriner hekim kontrolünde ishal tanısı alındı, ardından dışkı kıvamı ile gT skorları değerlendirmeye tabi tutuldu. Bilgi onam formu ile olgular dâhil edildi. Yine kurkumin dozajlanmasında, rasyonel veriler ele alındı. Zonulin ölçümlerinde Sandwich ELİSA kullanıldı. Bulgular: Dışkı skoru≥2-3 olan buzağılarda diyare pozitif kabul edildi. Dışkı skorlarında sağaltım sonrası, çıkış değerlerine göre belirgin istatistiksel farklılık (p<0,001) mevcuttu. Yine sağaltım sonrası gT skorları, öncesine oranla belirgin istatistiksel farklılık (p<0,001) göstermekteydi. İshalli buzağılarda ortalama zonulin (ng/mL) değerleri, sağaltım öncesi yüksek iken (56,54), kurkuminin oral uygulanması sonrası istatistiksel olarak belirgin şekilde (p<0,001) azalma (31,95) seyri gösterdi. Sonuç: Elde edilen veriler kurkuminin geçirgen bağırsağı azaltmada etkili olabileceğine delalet olabilir.
Anahtar Kelimeler: Buzağı; dışkı; gizli temizlik; kurkumin; zonulin
Amaç: Zerdeçal, Curcuma longa L.'den ekstrakte edildiği hâli ile antiinflamatuar, antibakteriyel, antiviral, antikanser, antioksidan ve koksidiyostatik aktivitelere sahiptir. Muhtemelen üzerinde en sıklıkla çalışılan, doğal bileşiklerden biridir. Geviş getiren hayvanlarda, geçirgen bağırsağa yönelik çok az çalışma mevcuttur. Bu araştırmada, ishalli buzağılarda geçirgen bağırsak (zonulin seviyeleri), dışkı kıvamı ile gizli temizlik (gT) skoru üzerine etkilerinin araştırılması özgün değere ve amaca yönelik çıkarımlardır. Gereç ve Yöntemler: Sahada aktif çalışan veteriner hekimleri bünyesinde barındıran, 4 farklı çiftlik ile irtibat kuruldu, temas noktasında tüm çiftlik çalışanlarından anamnez alındı. Bunun yani sıra gerek örneklem temininde, gerekse kan tahlillerinin alınmasında destek ve mukavemet tedariği mümkün kılındı. Her 4 çiftlikten toplamda n=19 buzağıda öncelikle veteriner hekim kontrolünde ishal tanısı alındı, ardından dışkı kıvamı ile gT skorları değerlendirmeye tabi tutuldu. Bilgi onam formu ile olgular dâhil edildi. Yine kurkumin dozajlanmasında, rasyonel veriler ele alındı. Zonulin ölçümlerinde Sandwich ELİSA kullanıldı. Bulgular: Dışkı skoru≥2-3 olan buzağılarda diyare pozitif kabul edildi. Dışkı skorlarında sağaltım sonrası, çıkış değerlerine göre belirgin istatistiksel farklılık (p<0,001) mevcuttu. Yine sağaltım sonrası gT skorları, öncesine oranla belirgin istatistiksel farklılık (p<0,001) göstermekteydi. İshalli buzağılarda ortalama zonulin (ng/mL) değerleri, sağaltım öncesi yüksek iken (56,54), kurkuminin oral uygulanması sonrası istatistiksel olarak belirgin şekilde (p<0,001) azalma (31,95) seyri gösterdi. Sonuç: Elde edilen veriler kurkuminin geçirgen bağırsağı azaltmada etkili olabileceğine delalet olabilir.
Anahtar Kelimeler: Buzağı; dışkı; gizli temizlik; kurkumin; zonulin
ABSTRACT
Objective: Turmeric, as extracted from Curcuma longa L., has anti-inflammatory, antibacterial, antiviral, anticancer, antioxidant and coccidiostatic activities. It is probably one of the most frequently studied natural preparations. Although there are some studies in ruminant animals, it was assumed to be of original value since it was not met with another study investigating its effects on leaky gut (zonulin levels), fecal consistency and latent cleansing score in calves with diarrhea, as is the subject of this article, in direct proportion to the purpose. Material and Methods: Contact was made with 4 different farms that included veterinarians working actively in the field; anamnesis was taken from all farm workers at the point of contact. In addition, it has been made possible to provide support and strength both in obtaining samples and taking blood tests. A total of n=19 calves from each 4 farms were first diagnosed with diarrhea under veterinarian control, and then fecal consistency and latent cleanliness scores were evaluated. Cases were included within the information consent form. Again, rational data on curcumin dosing was discussed. Sandwich ELISA was used for zonulin measurements. Results: Diarrhea was considered positive in calves with a stool score ≥2-3. There was a significant statistical difference (p<0.001) in stool scores after treatment compared to output values. Again, post-treatment gT scores showed a significant statistical difference (p<0.001) compared to before. While the average zonulin (ng/mL) values in calves with diarrhea were high before treatment (56.54), it showed a statistically significant (p<0.001) decrease (31.95) after oral administration of curcumin. Conclusion: The data obtained may indicate that curcumin may be effective in reducing leaky gut.
Keywords: Calf; feces; latent cleansing; curcumin; zonulin
Objective: Turmeric, as extracted from Curcuma longa L., has anti-inflammatory, antibacterial, antiviral, anticancer, antioxidant and coccidiostatic activities. It is probably one of the most frequently studied natural preparations. Although there are some studies in ruminant animals, it was assumed to be of original value since it was not met with another study investigating its effects on leaky gut (zonulin levels), fecal consistency and latent cleansing score in calves with diarrhea, as is the subject of this article, in direct proportion to the purpose. Material and Methods: Contact was made with 4 different farms that included veterinarians working actively in the field; anamnesis was taken from all farm workers at the point of contact. In addition, it has been made possible to provide support and strength both in obtaining samples and taking blood tests. A total of n=19 calves from each 4 farms were first diagnosed with diarrhea under veterinarian control, and then fecal consistency and latent cleanliness scores were evaluated. Cases were included within the information consent form. Again, rational data on curcumin dosing was discussed. Sandwich ELISA was used for zonulin measurements. Results: Diarrhea was considered positive in calves with a stool score ≥2-3. There was a significant statistical difference (p<0.001) in stool scores after treatment compared to output values. Again, post-treatment gT scores showed a significant statistical difference (p<0.001) compared to before. While the average zonulin (ng/mL) values in calves with diarrhea were high before treatment (56.54), it showed a statistically significant (p<0.001) decrease (31.95) after oral administration of curcumin. Conclusion: The data obtained may indicate that curcumin may be effective in reducing leaky gut.
Keywords: Calf; feces; latent cleansing; curcumin; zonulin
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