.: ORİJİNAL ARAŞTIRMA
Kaptoprilin Hücre Hasarı ve Karaciğer Hasarı Üzerine Etkileri
Effects of Captopril on Cell Damage and Liver Damage
Turkiye Klinikleri J Med Sci. 2020;40(3):320-5
doi: 10.5336/medsci.2020-74422
Makale Dili: EN
Tam Metin
ABSTRACT
Objective: To evaluate the effects of captopril (CAP), an angiotensin converting enzyme inhibitor (ACEI), on oxidative stress, liver enzymes, cardiovascular damage, and micronucleus (MN) frequency in streptozotocin-induced diabetes mellitus (STZ-DM). Material and Methods: A total of 24 young Sprague-Dawley male rats were divided into three groups as control, STZ, and STZ+CAP. The rats with DM, induced by way of a single dose injection of STZ (45 mg/kg), received CAP 50 mg/L/day for eight weeks. At the end of this period, liver and cardiovascular damage was evaluated from the level of creatine kinase (CK), creatine kinase isoenzyme-MB (CK-MB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) and the level of lipid peroxidation was evaluated using the thiobarbituric acid reactive substances (TBARS), glutathione (GSH), and total thiol content (T-SH). The MN frequency was also examined. Results: STZ was found to cause significant increases in the TBARS and LDH levels, ALT and AST activity, and MN frequency and a decrease in the GSH levels, while CAP increased T-SH production and decreased LDH activity and MN frequency. However, CAP increased the activities of ALT and AST. Conclusion: Our study results suggest that CAP produces antioxidant effects in rats with STZinduced DM and long-term use of CAP can correct the complications of diabetes at the cellular level, such as cell and tissue damage, as well as causing liver damage. Based on these results, diabetes seems to affect the liver, kidneys, and heart to varying degrees, although the liver is the most extensively affected organ.
Keywords: Diabetes mellitus; captopril; oxidative stress; angiotensin converting enzyme inhibitor; liver damage
Objective: To evaluate the effects of captopril (CAP), an angiotensin converting enzyme inhibitor (ACEI), on oxidative stress, liver enzymes, cardiovascular damage, and micronucleus (MN) frequency in streptozotocin-induced diabetes mellitus (STZ-DM). Material and Methods: A total of 24 young Sprague-Dawley male rats were divided into three groups as control, STZ, and STZ+CAP. The rats with DM, induced by way of a single dose injection of STZ (45 mg/kg), received CAP 50 mg/L/day for eight weeks. At the end of this period, liver and cardiovascular damage was evaluated from the level of creatine kinase (CK), creatine kinase isoenzyme-MB (CK-MB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) and the level of lipid peroxidation was evaluated using the thiobarbituric acid reactive substances (TBARS), glutathione (GSH), and total thiol content (T-SH). The MN frequency was also examined. Results: STZ was found to cause significant increases in the TBARS and LDH levels, ALT and AST activity, and MN frequency and a decrease in the GSH levels, while CAP increased T-SH production and decreased LDH activity and MN frequency. However, CAP increased the activities of ALT and AST. Conclusion: Our study results suggest that CAP produces antioxidant effects in rats with STZinduced DM and long-term use of CAP can correct the complications of diabetes at the cellular level, such as cell and tissue damage, as well as causing liver damage. Based on these results, diabetes seems to affect the liver, kidneys, and heart to varying degrees, although the liver is the most extensively affected organ.
Keywords: Diabetes mellitus; captopril; oxidative stress; angiotensin converting enzyme inhibitor; liver damage
ÖZET
Amaç: Bir anjiyotensin dönüştürücü enzim inhibitörü (ACEİ) olan kaptopril (CAP)'in streptozotosin kaynaklı diyabetes mellitusta (STZ-DM) oksidatif stres, karaciğer enzimleri, kardiyovasküler hasar ve mikronükleus (MN) frekansı üzerindeki etkilerini değerlendirmek. Gereç ve Yöntemler: Toplam 24 genç Sprague-Dawley erkek sıçan kontrol, STZ ve STZ+CAP olmak üzere üç gruba ayrıldı. Tek doz STZ enjeksiyonuyla (45 mg/kg) indüklenen DM'li sıçanlar, sekiz hafta boyunca 50 mg/L CAP aldı. Bu sürenin sonunda, karaciğer ve kardiyovasküler hasar, kreatin kinaz (CK), kreatin kinaz izoenzim-MB (CK-MB), alanin aminotransferaz (ALT), aspartat aminotransferaz (AST) ve laktat dehidrojenaz (LDH) seviyeleri değerlendirildi. Lipit peroksidasyon seviyesi, tiobarbitürik asit reaktif maddeler (TBARS), glutatyon (GSH) ve toplam tiol içeriği (T-SH) kullanılarak değerlendirildi. MN frekansı incelendi. Bulgular: STZ'nin TBARS ve LDH seviyeleri, ALT ve AST aktivitesi ve MN frekansında önemli artışa ve GSH seviyesinde azalmaya neden olduğu, CAP'in T-SH üretimini artırdığı, LDH aktivitesini ve MN frekansını azalttığı bulundu. Ancak CAP, ALT ve AST'nin aktivitelerini artırdı. Sonuç: Çalışma sonuçlarımız, CAP'in STZ ile indüklenen DM'li sıçanlarda antioksidan etki sağladığı ve CAP'in uzun süreli kullanımı diyabetin hücre ve doku hasarı gibi hücresel düzeydeki komplikasyonlarını düzeltirken karaciğerde de hasara yol açabileceğini düşündürmektedir. Bu sonuçlara dayanarak, diyabet karaciğer, böbrek ve kalbi değişen derecelerde etkilemekte, ancak karaciğer en çok etkilenen organdır.
Anahtar Kelimeler: Diabetes mellitus; kaptopril; oksidatif stres; anjiyotensin dönüştürücü enzim inhibitörü; karaciğer hasarı
Amaç: Bir anjiyotensin dönüştürücü enzim inhibitörü (ACEİ) olan kaptopril (CAP)'in streptozotosin kaynaklı diyabetes mellitusta (STZ-DM) oksidatif stres, karaciğer enzimleri, kardiyovasküler hasar ve mikronükleus (MN) frekansı üzerindeki etkilerini değerlendirmek. Gereç ve Yöntemler: Toplam 24 genç Sprague-Dawley erkek sıçan kontrol, STZ ve STZ+CAP olmak üzere üç gruba ayrıldı. Tek doz STZ enjeksiyonuyla (45 mg/kg) indüklenen DM'li sıçanlar, sekiz hafta boyunca 50 mg/L CAP aldı. Bu sürenin sonunda, karaciğer ve kardiyovasküler hasar, kreatin kinaz (CK), kreatin kinaz izoenzim-MB (CK-MB), alanin aminotransferaz (ALT), aspartat aminotransferaz (AST) ve laktat dehidrojenaz (LDH) seviyeleri değerlendirildi. Lipit peroksidasyon seviyesi, tiobarbitürik asit reaktif maddeler (TBARS), glutatyon (GSH) ve toplam tiol içeriği (T-SH) kullanılarak değerlendirildi. MN frekansı incelendi. Bulgular: STZ'nin TBARS ve LDH seviyeleri, ALT ve AST aktivitesi ve MN frekansında önemli artışa ve GSH seviyesinde azalmaya neden olduğu, CAP'in T-SH üretimini artırdığı, LDH aktivitesini ve MN frekansını azalttığı bulundu. Ancak CAP, ALT ve AST'nin aktivitelerini artırdı. Sonuç: Çalışma sonuçlarımız, CAP'in STZ ile indüklenen DM'li sıçanlarda antioksidan etki sağladığı ve CAP'in uzun süreli kullanımı diyabetin hücre ve doku hasarı gibi hücresel düzeydeki komplikasyonlarını düzeltirken karaciğerde de hasara yol açabileceğini düşündürmektedir. Bu sonuçlara dayanarak, diyabet karaciğer, böbrek ve kalbi değişen derecelerde etkilemekte, ancak karaciğer en çok etkilenen organdır.
Anahtar Kelimeler: Diabetes mellitus; kaptopril; oksidatif stres; anjiyotensin dönüştürücü enzim inhibitörü; karaciğer hasarı
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