.: ORİJİNAL ARAŞTIRMA
Evaluation of the Role of Paraoxonase1 Q192R Polymorphism in Coronary Artery Disease
Koroner Arter Hastalığında Paraoksonaz1 Q192R Polimorfizminin Rolünün Değerlendirilmesi
Turkiye Klinikleri J Cardiovasc Sci. 2020;32(1):9-13
doi: 10.5336/cardiosci.2019-71621
Makale Dili: EN
Tam Metin
ABSTRACT
Objetcive: Gene factors play an important role in coronary artery disease (CAD). Atherosclerosis is the most common process of developing CAD. Low density lipoprotein (LDL) oxidation has an essential role in the process of atherosclerosis. The paraoxonase 1 (PON1) enzyme that presents in the HDL structure inhibits LDL oxidation. A number of studies have been conducted around the world, investigating the relation between PON1 gene polymorphism and CAD. Different results have been obtained in various populations; therefore, these findings cannot be generalized to the Iranian population. Consequently, a comprehensive study of the prevalence of PON1 Q192R polymorphism is required. Material and Methods: In the present investigation, the study population consisted of 150 subjects, including 102 CAD patients and 48 healthy controls. The Q192R polymorphism of paraoxonase 1 was evaluated in general and also by ethnicity, employing the PCR-RFLP method. Results: In general, there was no significant difference (p>0.05) in the distribution of QQ, QR, and RR genotypes between patients and controls. In the study by ethnicity, in the Arab ethnic group, in patients with CAD, frequency of QQ genotype was significantly higher, while RR genotype was considerably lower than controls (p<0.05). Therefore, QQ genotype could be considered as a risk factor and RR genotype as a protective factor against CAD in the Arab race. Conclusion: The Q192R polymorphism of paraoxonase 1 in Arab ethnic group had a significant correlation with CAD. However, no overall association was found between this polymorphism and CAD.
Keywords: Q192R polymorphism of paraoxonase 1; coronary artery disease; Genetic difference
Objetcive: Gene factors play an important role in coronary artery disease (CAD). Atherosclerosis is the most common process of developing CAD. Low density lipoprotein (LDL) oxidation has an essential role in the process of atherosclerosis. The paraoxonase 1 (PON1) enzyme that presents in the HDL structure inhibits LDL oxidation. A number of studies have been conducted around the world, investigating the relation between PON1 gene polymorphism and CAD. Different results have been obtained in various populations; therefore, these findings cannot be generalized to the Iranian population. Consequently, a comprehensive study of the prevalence of PON1 Q192R polymorphism is required. Material and Methods: In the present investigation, the study population consisted of 150 subjects, including 102 CAD patients and 48 healthy controls. The Q192R polymorphism of paraoxonase 1 was evaluated in general and also by ethnicity, employing the PCR-RFLP method. Results: In general, there was no significant difference (p>0.05) in the distribution of QQ, QR, and RR genotypes between patients and controls. In the study by ethnicity, in the Arab ethnic group, in patients with CAD, frequency of QQ genotype was significantly higher, while RR genotype was considerably lower than controls (p<0.05). Therefore, QQ genotype could be considered as a risk factor and RR genotype as a protective factor against CAD in the Arab race. Conclusion: The Q192R polymorphism of paraoxonase 1 in Arab ethnic group had a significant correlation with CAD. However, no overall association was found between this polymorphism and CAD.
Keywords: Q192R polymorphism of paraoxonase 1; coronary artery disease; Genetic difference
ÖZET
Amaç: Gen faktörleri koroner arter hastalığında (KAH) önemli rol oynar. Ateroskleroz KAH gelişiminin en yaygın sürecidir. Düşük yoğunluklu protein (LDL) oksidasyonunun ateroskleroz sürecinde önemli rolü vardır. Yüksek yoğunluklu protein (HDL) yapısında yer alan paraoksonaz 1 (PON1) enzimi LDL oksidasyonunu inhibe eder. Dünyada PON1 gen polimorfizmi ile KAH arasındaki ilişkiyi inceleyen çok sayıda çalışma yapılmıştır. Çeşitli populasyonlarda farklı sonuçlar elde edilmiştir; bu nedenle bu bulgular İran populasyonuna genellenemez. Sonuç olarak, PON1 Q192R polimorfizminin prevalansı ile ilgili kapsamlı bir çalışma yapılması gereklidir. Gereç ve Yöntemler: Bu araştırmada, çalışma populasyonu 150 olguyu içeriyordu (KAH olan 102 hasta ve 48 sağlıklı kontrol). Paraoksonazın Q192R polimorfizmi PCR-RFLP yöntemi kullanılarak genelde ve etnisiteye göre değerlendirildi. Bulgular: Genelde, hastalarla kontroller arasında QQ, QR ve RR genotiplerinin dağılımı açısından anlamlı fark yoktu (p>0.05). Etnisiteye göre ise Arap etnik grubunda KAH olanlarda QQ genotpinin sıklığı anlamlı olarak daha yüksekti, RR genotipi konrollere göre anlamlı olarak daha düşüktü (p<0.05). Bu nedenle, Arap toplumunda QQ genotipi bir risk faktörü olarak düşünülebilir ve RR genotipi koruyucu bir faktör olarak düşünülebilir. Sonuç: Arap etnik grubunda paraoksonaz 1?in Q192R polimorfizmi KAH ile anlamlı olarak korele idi. Fakat bu polimorfizm ile KAH arasında genel bir ilişki bulunmadı.
Anahtar Kelimeler: Q192R polimorfizmi; koroner arter hastalığı; genetik farklılık
Amaç: Gen faktörleri koroner arter hastalığında (KAH) önemli rol oynar. Ateroskleroz KAH gelişiminin en yaygın sürecidir. Düşük yoğunluklu protein (LDL) oksidasyonunun ateroskleroz sürecinde önemli rolü vardır. Yüksek yoğunluklu protein (HDL) yapısında yer alan paraoksonaz 1 (PON1) enzimi LDL oksidasyonunu inhibe eder. Dünyada PON1 gen polimorfizmi ile KAH arasındaki ilişkiyi inceleyen çok sayıda çalışma yapılmıştır. Çeşitli populasyonlarda farklı sonuçlar elde edilmiştir; bu nedenle bu bulgular İran populasyonuna genellenemez. Sonuç olarak, PON1 Q192R polimorfizminin prevalansı ile ilgili kapsamlı bir çalışma yapılması gereklidir. Gereç ve Yöntemler: Bu araştırmada, çalışma populasyonu 150 olguyu içeriyordu (KAH olan 102 hasta ve 48 sağlıklı kontrol). Paraoksonazın Q192R polimorfizmi PCR-RFLP yöntemi kullanılarak genelde ve etnisiteye göre değerlendirildi. Bulgular: Genelde, hastalarla kontroller arasında QQ, QR ve RR genotiplerinin dağılımı açısından anlamlı fark yoktu (p>0.05). Etnisiteye göre ise Arap etnik grubunda KAH olanlarda QQ genotpinin sıklığı anlamlı olarak daha yüksekti, RR genotipi konrollere göre anlamlı olarak daha düşüktü (p<0.05). Bu nedenle, Arap toplumunda QQ genotipi bir risk faktörü olarak düşünülebilir ve RR genotipi koruyucu bir faktör olarak düşünülebilir. Sonuç: Arap etnik grubunda paraoksonaz 1?in Q192R polimorfizmi KAH ile anlamlı olarak korele idi. Fakat bu polimorfizm ile KAH arasında genel bir ilişki bulunmadı.
Anahtar Kelimeler: Q192R polimorfizmi; koroner arter hastalığı; genetik farklılık
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