Objective: The objective of the study was to determine mean of treatment period, some liver enzyme levels and blood parameters in dogs with TVT that were administered standard vincristine sulphate and administered propolis by different routes. Material and Methods: A total of 24 dogs with TVT (9 males, 15 females), irrespective of breed and age, were included in the study. The study was divided into four groups: a control group (n=6, receiving vincristine sulfate), an oral group (n=7, receiving vincristine sulfate + 100 mg/kg raw propolis daily), a local group (n=5, receiving vincristine sulfate + 200 mg/ml raw propolis daily), and an oral+local group (n=6, receiving vincristine sulfate + 100 mg/kg raw propolis daily, orally and 200 mg/ml raw propolis daily, locally). Weekly clinical examinations were conducted, and smears and blood samples were collected. Staining and histopathological examination were performed using the Diff-quick staining test. Results: Although there are not significant differences, mean treatment weeks in all groups that added propolis were lower than control group. Neutropenia, neutrophilia, leukopenia, and leukocytosis were confirmed most of the treated dogs. Alanine transaminase, aspartate transaminaz, alkaline phosphatase, gamma glutamyltransferase and lactate dehydrogenase levels were in the reference values in control and experimental groups. Conclusion: The levels of liver enzymes in all groups were within the reference range, indicating that vincristine sulfate did not cause hepatotoxicity. TVT treatment periods in groups used propolis were shorter than the standard vincristine sulphate therapy. Antitumoral effects of propolis should much more extensively study with animal and clinical experiments.
Keywords: Transmissible venereal tumor; vincristine; propolis
Amaç: Bu çalışmada, vinkristin sülfat ile standart medikal tedavi alan TVT'li köpeklerde, farklı yollarla propolis uygulamasının, iyileşme sürelerine, bazı karaciğer enzim düzeyleri ve kan parametrelerine etkisinin incelenmesi amaçlandı. Gereç ve Yöntemler: Çalışmaya 24 TVT'li köpek (9 erkek, 15 dişi), ırk ve yaş gözetmeksizin dahil edildi. Çalışma, kontrol grubu (n=6, sadece vinkristin sülfat), oral grup (n=7, vinkristin sülfat+ ham propolis 100 mg/kg, günde bir), lokal grup (n=5, vinkristin sülfat+ ham propolis 200 mg/ml, günde bir) ve oral+lokal grup (n=6, vinkristin sülfat + ham propolis 100 mg/kg, günde bir, oral+ham propolis 200 mg/ml günde bir, lokal) olarak dört gruba ayrılarak yapıldı. Her hafta, klinik kontrolden sonra smear ve kan alındı. Diff-quick boyama testi ile boyama ve histopatolojik inceleme yapıldı. Bulgular: İstatistiki olarak fark bulunmamakla birlikte propolis ilave edilen tüm gruplarda iyileşme süresinin daha kısa olduğu görüldü. Nötropeni, nötrofili ile lökopeni ve lökositoz tedavi edilen köpeklerin büyük çoğunluğunda tespit edildi. Alanin aminotransferaz, aspartat aminotransferaz, alkalen fosfataz, gama glutamil transferaz ve laktat dehidrojenaz düzeylerinin tüm gruplarda referans değer aralığında olduğu görüldü. Sonuç: Kontrol ve deney grubu köpeklerin karaciğer enzim düzeyleri referans değer aralığında olduğu görüldü ve vinkristin sülfatın hepatotoksisiteye neden olmadığı kanısına varıldı. TVT'nin standart vinkristin sülfat ile tedavisinde propolis ilave edilen gruplarda iyileşme daha hızlı olmuştur. Propolisin antitümoral etkisi daha detaylı olarak hayvan ve klinik çalışmalarla yapılmalıdır.
Anahtar Kelimeler: Bulaşıcı zührevi tümör; vinkristin; propolis
- Kose AM, Cizmeci SU, Aydin I, Dinc DA, Maden M, Kanat O. Disseminated metastatic transmissible venereal tumour in a bitch. Eurasian J Vet Sci. 2013;29(1):53-7. [Link]
- Hantrakul S, Klangkaew N, Kunakornsawat S, Tansatit T, Poapolathep A, Kumagai S, et al. Clinical pharmacokinetics and effects of vincristine sulfate in dogs with transmissible venereal tumor (TVT). J Vet Med Sci. 2014;76(12):1549-53. [Crossref] [PubMed] [PMC]
- Amaral AS. Transmissible canine venereal tumor: critérios cytological de malignidade e characterization cytomorphological correlacionada a imunocitoquímica e lesões de DNA [PhD thesis]. Botucatu: Faculdade de Medicina Veterinary e Zootecnia, Universidade Estadual Paulista; 2005. [Cited: November 22, 2023]. Available from: [Link]
- Bassani-Silva S, Sforcin JM, Amaral AS, Gaspar LFJ, Rocha NS. Propolis effect in vitro on canine transmissible venereal tumor cells. Rev Port Cienc Vet. 2007;102:261-5. [Link]
- Gaspar LF, Ferreira I, Colodel MM, Brandão CV, Rocha NS. Spontaneous canine transmissible venereal tumor: cell morphology and influence on P-glycoprotein expression. Turk J Vet Anim Sci. 2010;34(5):447-54. [Link]
- Yadav A, Bugalia NS, Pandey AK. Haemato-biochemical and therapeutic evaluation of doxorubicin and vincristine in canine transmissible venereal tumour. Indian J Anim Reprod. 2018;39(2):40-2. [Link]
- Souza JVDA, Gonçalves FDM, Lira-Junior ACOG, Escodro PB, Câmara DR, Notomi MK. Vincristine sulfate treatment influence on kidney function of female dogs with transmissible venereal tumor. Braz J Vet Res Anim Sci. 2022(59):e192646. [Crossref]
- Kabuusu RM, Kumthekar S, Tang J, Alexander R, Sylvester W, Lanza- Perea M, et al. Clinicopathological changes during canine transmissible venereal tumor treatment with vincristine. Indian J Vet Pathol. 2019;43(2):132-5. [Crossref]
- Braz PH, Marinho CP. Comparison between hematological and biochemical changes caused by conventional and metronomic chemotherapies in the treatment of canine transmissible venereal tumor. Pesq Vet Bras. 2021;41:e06575. [Crossref]
- Kumar VA, Kumari KN, Kumar KS, Kumar VG, Lakshman M. Effect of vincristine chemotherapy in TVT affected dogs. J Pharm Innov. 2018;7(4):163-6. [Link]
- Oruç HH, Çayci M, Sorucu A, Uzabacı E, Nyandwi R. Characterization of commercially available propolis products in Turkey based on individual phenolic compounds. J Apic Res. 2023;62(5):1225-32. [Crossref]
- Catchpole O, Mitchell K, Bloor S, Davis P, Suddes A. Antiproliferative activity of New Zealand propolis and phenolic compounds vs human colorectal adenocarcinoma cells. Fitoterapia. 2015;106:167-74. [Crossref] [PubMed]
- Doğan H, Silici S, Ozcimen AA. Biological effects of propolis on cancer. TURJAF. 2020;8(3):573-9. [Crossref]
- Chiu HF, Han YC, Shen YC, Golovinskaia O, Venkatakrishnan K, Wang CK. Chemopreventive and chemotherapeutic effect of propolis and its constituents: a mini-review. J Cancer Prev. 2020;25(2):70-8. [Crossref] [PubMed] [PMC]
- Altabbal S, Athamnah K, Rahma A, Wali AF, Eid AH, Iratni R, et al. Propolis: a detailed insight of its anticancer molecular mechanisms. Pharmaceuticals (Basel). 2023;16(3):450. [Crossref] [PubMed] [PMC]
- Hosoya T, Tsuchiya I, Ohta T, Benhanifia M, Kumazawa S. Composition of algerian propolis, plant origin, and its antiangiogenic activity in vitro. Molecules. 2021;26(21):6510. [Crossref] [PubMed] [PMC]
- Sawicka D, Car H, Borawska MH, Nikliński J. The anticancer activity of propolis. Folia Histochem Cytobiol. 2012;50(1):25-37. [Crossref] [PubMed]
- Wen X, Lin ZQ, Liu B, Wei YQ. Caspase-mediated programmed cell death pathways as potential therapeutic targets in cancer. Cell Prolif. 2012;45(3):217-24. [Crossref] [PubMed] [PMC]
- Park SI, Ohta T, Kumazawa S, Jun M, Ahn MR. Korean propolis suppresses angiogenesis through inhibition of tube formation and endothelial cell proliferation. Nat Prod Commun. 2014;9(4):555-60. [Crossref] [PubMed]
- Patel S. Emerging adjuvant therapy for cancer: propolis and its constituents. J Diet Suppl. 2016;13(3):245-68. [Crossref] [PubMed]
- Wang M, Jiang X. SUMOylation of vascular endothelial growth factor receptor 2 inhibits the proliferation, migration, and angiogenesis signaling pathway in non-small cell lung cancer. Anticancer Drugs. 2020;31(5):492-9. [Crossref] [PubMed]
- Szliszka E, Krol W. The role of dietary polyphenols in tumor necrosis factor-related apoptosis inducing ligand (TRAIL)-induced apoptosis for cancer chemoprevention. Eur J Cancer Prev. 2011;20(1):63-9. [Crossref] [PubMed]
- Sforcin JM. Propolis and the immune system: a review. J Ethnopharmacol. 2007;113(1):1-14. [Crossref] [PubMed]
- Daikh A, Segueni N, Dogan NM, Arslan S, Mutlu D, Kıvrak I, et al. Comparative study of antibiofilm, cytotoxic activity and chemical composition of Algerian propolis. J Apic Res. 2020;59(2):160-9. [Crossref]
- Madania RN, Jayawardhita AAG, Kendran AAS. Aktivitas alanine aminotransferase dan aspartate aminotransferase pada anjing penderita transmissible venereal tumor yang diobati dengan vincristine. Indones Med Veterinus. 2019;8(3):366-75. [Link]
- Sahlan M, Rizka Alia Hapsari N, Diah Pratami K, Cahya Khayrani A, Lischer K, Alhazmi A, et al. Potential hepatoprotective effects of flavonoids contained in propolis from South Sulawesi against chemotherapy agents. Saudi J Biol Sci. 2021;28(10):5461-8. [Crossref] [PubMed] [PMC]
- Rezaei MS, AziziS, Shahheidaripour S, Rostami S. Primary oral and nasal transmissible venereal tumor in a mix-breed dog. Asian Pac J Trop Biomed. 2016;6(5):443-5. [Crossref]
- Orsolić N, Sver L, Terzić S, Tadić Z, Basić I. Inhibitory effect of water-soluble derivative of propolis and its polyphenolic compounds on tumor growth and metastasizing ability: a possible mode of antitumor action. Nutr Cancer. 2003;47(2):156-63. [Crossref] [PubMed]
- Oruç HH, Sorucu A, Ünal HH, Aydın L. Effects of season and altitude on biological active certain phenolic compounds levels and partial standardization of propolis. A Ü Vet Fak. Derg. 2017;64:13-20. [Crossref]
- Bankova VS, De Castro SL, Marcucci MC. Propolis: recent advances in chemistry and plant origin. Apidologie. 2000;31(1):3-15. [Crossref]
- Sorucu A, Oruç HH. Determination of biologically active phenolic compounds in propolis by LC-MS/MS according to seasons and altitudes. J Food Meas Charact. 2019;13:2461-9. [Crossref]
- Abubakar MB, Abdullah WZ, Sulaiman SA, Ang BS. Polyphenols as key players for the antileukaemic effects of propolis. Evid Based Complement Alternat Med. 2014;2014:371730. [Crossref] [PubMed] [PMC]
- Or?olić N, Jazvin?ćak Jembrek M. Molecular and cellular mechanisms of propolis and its polyphenolic compounds against cancer. Int J Mol Sci. 2022;23(18):10479. [Crossref] [PubMed] [PMC]
- Kumar VA, Kumari KN, Kumar KS, Kumar VG, Lakshman M. Hemato-biochemical changes in transmissible venereal tumours (TVT) affected dogs. J Pharm Innov. 2017;6(12):313-5. [Link]
- Prabha N, Kumar A, Kumar Tiwari D, Chaudhary RN, Sindhur A. Effect of vincristine sulphate on haemato-biochemical profile of healthy dogs. Haryana Vet. 2019;58(1):15-9. [Link]
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