Amaç: Crithmum maritimum L. (C. maritimum L.) antimikrobiyal, antioksidan ve rejeneratif özellikleri olan halofit bir bitkidir. Bu in vitro çalışmada amacımız, bir antioksidan olarak C. Maritimum L.'nin, insan periodontal ligament fibroblast hücrelerine proliferatif etkisini değerlendirmektir. Gereç ve Yöntemler: Periodontal ligament fibroblast hücreleri 96 kuyulu plakalara ekildi. Çalışma grupları, 4, 5, 6, 7, 8, 10, 20, 50, 100 μg/mL C. maritimum L. konsantrasyonlarına 24, 48 ve 72 saat maruz bırakıldı. C. maritimum L. uygulamasından sonra, hücre proliferasyonunu değerlendirmek için XTT analizi gerçekleştirildi. Analiz için kuyulardaki medyum aspire edildi, 100 μL taze medyum ve 50 μL XTT reaksiyon solüsyonu eklendi. 4 saat sonra tüm kuyuların absorbansı 450 nm ve 630 nm'de ELISA okuyucuda okundu ve bu değerler arasındaki fark hesaplanarak net absorbans değerleri belirlendi. Proliferasyon ortalamalarını hesaplamak için test gruplarının absorbans değerlerinin yüzdesi kontrol grubunun yüzdesi ile karşılaştırıldı. Kontrol grubu ile diğer gruplar arasındaki karşılaştırmalar Mann-Whitney-U testi ile yapıldı ve p<0,05 anlamlı olarak kabul edildi. Bulgular: Yirmi dört saat sonra 20 ve 50 μg/mL C. maritimum L. konsantrasyonları uygulanan gruplar ile 48 saat sonunda 5, 6, 10, 20 μg/mL C. maritimum L. uygulanan grupların hücre proliferasyonu kontrol grubundaki hücrelere göre istatistiksel olarak anlamlı bir şekilde arttı (p<0,05). Sonuç: Bu sonuçlar, C. maritimum L.'nin periodontal dokuların rejenerasyonunda rol oynayan periodontal ligament fibroblast hücrelerinin proliferasyonunu artırmasından dolayı periodontal sağlığın korunması amacıyla kullanılabileceğini göstermiştir.
Anahtar Kelimeler: Antioksidanlar; peryodontal bağ; primer hücre kültürü
Objective: Crithmum maritimum L. (C. maritimum L.) is a halophyte plant with antimicrobial, antioxidant and regenerative properties. The aim of this in vitro study was to evaluate the proliferation effects of C. maritimum L. as an antioxidant on human periodontal ligament fibroblast cells. Material and Methods: Periodontal ligament fibroblast cells were seeded in 96-well plates. The study groups were exposed to various concentrations of C. maritimum L., as 4, 5, 6, 7, 8, 10, 20, 50 and 100 μg/mL for 24, 48 and 72 hours. After treatments, the XTT assay was performed to evaluate cell proliferation. To analysis, the medium in the wells was aspirated, 100 μL fresh medium and 50 μL XTT reaction solution were added. After 4 hours, the absorbance of all wells was read at 450 nm and 630 nm with ELISA and the net absorbance values were determined by calculating the difference between these values. The percentage of the absorbance values of the test groups was compared with the percentage of the control group to calculate the average of proliferation. Mann-Whitney- U test was used in comparison between control and other groups and p<0.05 was considered significant. Results: After 24 and 48 hours, cell proliferation of groups treated with 20, 50 μg/mL and 5, 6, 10 and 20 μg/mL C. maritimum L. concentrations respectively were increased statistically significant compared to the control cells (p<0.05). Conclusion: These results indicated that C. maritimum L. could be used to ensure the periodontal healing due to increasing the proliferation of periodontal ligament fibroblast cells that play a role in the regeneration of periodontal tissues.
Keywords: Antioxidants; periodontal ligament; primary cell culture
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