.: ORİJİNAL ARAŞTIRMA
Berberin-Klorürün Meme Kanseri Hücreleri Üzerindeki Sitotoksik Etkilerinin Değerlendirilmesi ve Antimikrobiyal Aktivite Analizi
Evaluation of Cytotoxic Effects of Berberine-Chloride in Breast Cancer Cells and Antimicrobial Activity Analysis
Turkiye Klinikleri J Med Sci. 2020;40(3):342-8
doi: 10.5336/medsci.2020-74815
Makale Dili: TR
Tam Metin
ÖZET
Amaç: Bu çalışmada, berberin-klorürün antikanser etkinliğinin, 2 farklı kanser hücre hattında (MCF-7 meme kanseri hücreleri ve M4A4 metastatik meme kanseri hücreleri) analiz edilmesi ve antibakteriyel etkinliğinin 5 farklı standart bakteri suşunda araştırılması amaçlanmıştır. Gereç ve Yöntemler: Çalışmada, MCF-7 meme kanseri hücreleri ve M4A4 metastatik meme kanseri hücreleri olmak üzere 2 hücre hattına karşı MTT analizi uygulanarak, berberin-klorürün zamana bağlı oluşturduğu inhibisyon araştırılmıştır. Ayrıca berberin-klorürün 6 standart suş [Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, 'methicillin resistant S. aureus (MRSA)', Pseudomonas aeruginosa ve Enterococcus faecalis) kullanılarak antibakteriyel etkisi broth mikrodilüsyon ve agar zon difüzyon yöntemleri ile analiz edilmiştir. Bulgular: MCF-7 hücreleri için hücre canlılığının %50 inhibe olduğu konsantrasyon, 72 saatlik berberin-klorür muamelesi sonrası 19,57 µM, M4A4 hücreleri için ise 77,7 µM olarak bulunmuştur. Broth mikrodilüsyon testinde minimum inhibitör konsantrasyonu değerleri, S. aureus, MRSA, E. coli, K. pneumoniae, P. aeruginosa ve E. faecalis için sırasıyla 0,49 µg/mL, 0,98 µg/mL, 0,0038 µg/mL, 3,91 µg/mL, 0,03 µg/mL ve 0,49 µg/mL olarak bulunmuştur. Agar kuyucuk difüzyon testinde zon inhibisyon değerleri S. aureus, MRSA, E. coli, K. pneumoniae, P. aeruginosa ve E. faecalis için sırasıyla 30 mm, 30 mm, 35 mm, 17 mm, 33 mm ve 20 mm olarak bulunmuştur. Sonuç: Çalışmada, berberinklorürün farklı hücre hatlarına antikanser etkisi ve yaygın olarak enfeksiyona sebep olan bakterilere karşı antibakteriyel etkinliği olduğu in vitro olarak gösterilmiştir.
Anahtar Kelimeler: Berberin-klorür; antibakteriyel; antikanser; sitotoksisite; MTT analizi
Amaç: Bu çalışmada, berberin-klorürün antikanser etkinliğinin, 2 farklı kanser hücre hattında (MCF-7 meme kanseri hücreleri ve M4A4 metastatik meme kanseri hücreleri) analiz edilmesi ve antibakteriyel etkinliğinin 5 farklı standart bakteri suşunda araştırılması amaçlanmıştır. Gereç ve Yöntemler: Çalışmada, MCF-7 meme kanseri hücreleri ve M4A4 metastatik meme kanseri hücreleri olmak üzere 2 hücre hattına karşı MTT analizi uygulanarak, berberin-klorürün zamana bağlı oluşturduğu inhibisyon araştırılmıştır. Ayrıca berberin-klorürün 6 standart suş [Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, 'methicillin resistant S. aureus (MRSA)', Pseudomonas aeruginosa ve Enterococcus faecalis) kullanılarak antibakteriyel etkisi broth mikrodilüsyon ve agar zon difüzyon yöntemleri ile analiz edilmiştir. Bulgular: MCF-7 hücreleri için hücre canlılığının %50 inhibe olduğu konsantrasyon, 72 saatlik berberin-klorür muamelesi sonrası 19,57 µM, M4A4 hücreleri için ise 77,7 µM olarak bulunmuştur. Broth mikrodilüsyon testinde minimum inhibitör konsantrasyonu değerleri, S. aureus, MRSA, E. coli, K. pneumoniae, P. aeruginosa ve E. faecalis için sırasıyla 0,49 µg/mL, 0,98 µg/mL, 0,0038 µg/mL, 3,91 µg/mL, 0,03 µg/mL ve 0,49 µg/mL olarak bulunmuştur. Agar kuyucuk difüzyon testinde zon inhibisyon değerleri S. aureus, MRSA, E. coli, K. pneumoniae, P. aeruginosa ve E. faecalis için sırasıyla 30 mm, 30 mm, 35 mm, 17 mm, 33 mm ve 20 mm olarak bulunmuştur. Sonuç: Çalışmada, berberinklorürün farklı hücre hatlarına antikanser etkisi ve yaygın olarak enfeksiyona sebep olan bakterilere karşı antibakteriyel etkinliği olduğu in vitro olarak gösterilmiştir.
Anahtar Kelimeler: Berberin-klorür; antibakteriyel; antikanser; sitotoksisite; MTT analizi
ABSTRACT
Objective: In this study, it was aimed to analyse the anti-cancer activity of berberine-chloride in two different cancer cell lines (MCF-7 breast cancer cells and M4A4 metastatic breast cancer cells) and to investigate the antibacterial activity in five different standard bacterial strains. Material and Methods: In the study, the timedependent inhibition by berberine chloride was investigated by applying MTT assay against two cell lines, which are MCF-7 breast cancer cells and M4A4 metastatic breast cancer cells. In addition, the anti-bacterial effect of berberine chloride was analysed by using six standard strains [Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, methicillin resistant S. aureus (MRSA), Pseudomonas aeruginosa and Enterococcus faecalis] with the broth microdilution and agar zone diffusion methods. Results: The concentration at which cell viability was 50% inhibited for MCF-7 cells after 72 hours of berberine chloride treatment was found to be 19.57 µM and 77.7 µM for M4A4 cells. Minimum inhibitory concentration values in broth microdilution test for S. aureus, MRSA, E. coli, K. pneumoniae, P. aeruginosa and E. faecalis, was found to be 0.49 µg/mL, 0.98 µg/mL, 0.0038 µg/mL 3.91 µg/mL, 0.03 µg/mL and 0.49 µg/mL respectively. Zone inhibition values in agar well diffusion test was found as 30 mm, 30 mm, 35 mm, 17 mm, 33 mm and 20 mm for S. aureus, MRSA, E. coli, K. pneumoniae, P. aeruginosa and E. faecalis, respectively. Conclusion: In the study, berberine-chloride anti-cancer effect on different cell lines and antibacterial activity against commonly infectious bacteria has been shown in-vitro.
Keywords: Berberine chloride; anti-bacterial; anti-cancer; cytotoxicity; MTT assay
Objective: In this study, it was aimed to analyse the anti-cancer activity of berberine-chloride in two different cancer cell lines (MCF-7 breast cancer cells and M4A4 metastatic breast cancer cells) and to investigate the antibacterial activity in five different standard bacterial strains. Material and Methods: In the study, the timedependent inhibition by berberine chloride was investigated by applying MTT assay against two cell lines, which are MCF-7 breast cancer cells and M4A4 metastatic breast cancer cells. In addition, the anti-bacterial effect of berberine chloride was analysed by using six standard strains [Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, methicillin resistant S. aureus (MRSA), Pseudomonas aeruginosa and Enterococcus faecalis] with the broth microdilution and agar zone diffusion methods. Results: The concentration at which cell viability was 50% inhibited for MCF-7 cells after 72 hours of berberine chloride treatment was found to be 19.57 µM and 77.7 µM for M4A4 cells. Minimum inhibitory concentration values in broth microdilution test for S. aureus, MRSA, E. coli, K. pneumoniae, P. aeruginosa and E. faecalis, was found to be 0.49 µg/mL, 0.98 µg/mL, 0.0038 µg/mL 3.91 µg/mL, 0.03 µg/mL and 0.49 µg/mL respectively. Zone inhibition values in agar well diffusion test was found as 30 mm, 30 mm, 35 mm, 17 mm, 33 mm and 20 mm for S. aureus, MRSA, E. coli, K. pneumoniae, P. aeruginosa and E. faecalis, respectively. Conclusion: In the study, berberine-chloride anti-cancer effect on different cell lines and antibacterial activity against commonly infectious bacteria has been shown in-vitro.
Keywords: Berberine chloride; anti-bacterial; anti-cancer; cytotoxicity; MTT assay
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