Journal of Traditional Medical Complementary Therapies

.: ORIGINAL RESEARCH
The Effect of Wet Cupping Therapy on Anxiety and Thiol/Disulfide Homeostasis in Premature Ovarian Failure: An Experimental Study
Prematür Overyan Yetmezlikli Ratlarda Yaş Kupa Terapisinin Anksiyete ve Tiyol/Disülfit Homeostazı Üzerine Etkileri: Deneysel Bir Çalışma
Hümeyra ÇELİKa, Ayhan ÇETİNKAYAa, Bihter Gökçe BOZATb, Selma ERDOĞAN DÜZCÜc, Murat ALIŞIKd, Özgür Mehmet YİSd
aDepartment of Physiology, Bolu Abant İzzet Baysal University Faculty of Medicine, Bolu, Türkiye
bDepartment of Interdisciplinary Neuroscience, Bolu Abant İzzet Baysal University Health Science Institute, Bolu, Türkiye
cDepartment of Pathology, Bolu Abant İzzet Baysal University Faculty of Medicine, Bolu, Türkiye
dDepartment of Biochemistry, Bolu Abant İzzet Baysal University Faculty of Medicine, Bolu, Türkiye
J Tradit Complem Med. 2022;5(1):12-21
doi: 10.5336/jtracom.2021-83703
Article Language: EN
Full Text
ABSTRACT
 Objective: The effect of wet cupping therapy on anxiety and thiol/disulfide homeostasis was evaluated in rats with premature ovarian failure (POF) induced by cyclophosphamide (CYC). Material and Methods: Twenty four Wistar Albino female rats were divided into control (n=6), POF (n=6), wet cupping (n=6), POF+wet cupping (n=6) groups. CYC at a dose of 200 mg/kg was injected intraperitoneally to POF and POF+wet cupping groups. One week later, wet cupping therapy was applied to wet cupping and POF+wet cupping groups for 5 minutes. The next day, Open Field Test and Elevated Plus Maze Test were applied to all experimental groups to evaluate anxiety. After sacrification, to assess pattern formation, endometrial thickness and ovarian reserve were measured with hematoxylin-eosin staining. Thiol/disulfide homeostasis and erythrocyte oxidized-reduced glutathione levels were measured in serum to measure oxidative stress. Results: Although there was no statistical significance, it was observed that anxiety decreased in POF+wet cupping compared to POF group. There was no change in thiol-disulfide values in POF+wet cupping compared to POF group (p>0.05). Conclusion: Wet cupping therapy did not reduce anxiety in the experimental POF model and did not shift the balance to positive in thiol/disulfide homeostasis in favor of thiols. However, the obtained graphics, numerical data and high standard deviation suggest that meaningful data could be obtained with more subjects.

Keywords: Cupping theraphy; cyclophosphamide; premature ovarian failure; anxiety; sulfydryl compounds
ÖZET
 Amaç: Siklofosfamid [cyclophosphamide (CYC)] ile indüklenmiş prematür overyan yetmezliği (POF) olan ratlarda yaş kupa terapisinin anksiyete ve tiyol/disülfid homeostazı üzerindeki etkileri değerlendirildi. Gereç ve Yöntemler: Yirmi dört adet Wistar Albino dişi rat kontrol (n=6), POF (n=6), yaş kupa (n=6), POF+yaş kupa (n=6) gruplarına ayrıldı. POF ve POF+yaş kupa gruplarına 200 mg/kg CYC intraperitoneal olarak enjekte edildi. Bir hafta sonra yaş kupa ve POF+yaş kupa gruplarına 5 dk süreyle kupa terapisi uygulandı. Ertesi gün, anksiyeteyi değerlendirmek için tüm deney gruplarına Açık Alan Testi ve Yükseltilmiş Artı Labirent Testi uygulandı. Sakrifikasyonun ardından model oluşumunu değerlendirmek için endometrial kalınlık ve over rezervi hematoksilen-eozin boyası ile ölçüldü. Oksidatif stresi değerlendirmek amacıyla toplanan serumlardan tiyol/disülpid homeostazı ve eritrosit okside-redükte glutatyon düzeyleri bakıldı. Bulgular: İstatistiksel olarak anlamlı olmamakla birlikte POF+yaş kupa grubunda POF grubuna göre anksiyetenin azaldığı görüldü. Tiyol-disülfid değerlerinde ise POF+yaş kupa grubunda POF grubuna göre bir farklılık bulunmadı (p>0,05). Sonuç: Yaş kupa terapisinin deneysel POF modelinde anksiyeteyi azaltmadığı ve tiyol/disulfit homeostazında dengeyi tiyoller yönünden pozitif balansa kaydırmadığı gözlemlenmiştir. Ancak elde edilen grafikler, sayısal veriler ve standart sapmanın yüksek oluşu daha fazla denek ile anlamlı sonuçların elde edilebileceğini düşündürmektedir.

Anahtar Kelimeler: Kupa terapisi; siklofosfamid; prematür ovaryan yetmezlik; anksiyete; sülfidril bileşikleri
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