Objective: This study has investigated the effects of walnut consumption on the serum lipids and especially small dense lowdensity lipoprotein (sdLDL) values of healthy volunteers. Material and Methods: Volunteers participating in this study were asked to consume 30 grams of walnuts per day. Walnuts were presented to individuals in packages and were asked to consume at breakfast every morning for 21 days. An short message service (SMS) reminder was made every day so that individuals would not forget to intake walnuts. The blood values of subjects were measured at the beginning and the end of the study. Serums were obtained from the collected blood, and blood lipid parameters were analyzed routinely in the biochemistry laboratory. The sdLDL parameter was analyzed by the ELISA method. Results: The effect of walnut consumption on the lipid parameters was investigated. It was found that regular walnut consumption has significantly decreased the LDL (p<0.001), total cholesterol (p<0.001), sdLDL (p<0.001), and HDL (p=0.008) parameters. A significant change was not observed for the triglyceride levels. Besides, there is a statistically significant difference in carbohydrate, linoleic acid, and a-linolenic acid intake compared to Turkey Nutrition and Health Research. There was no significant difference in the individuals' energy, cholesterol, monounsaturated fatty acid, and saturated fatty acid intakes Also, food consumption record form was obtained from individuals. Conclusion: In this study, supplementation walnut to the healthy volunteers' diet has reduced LDL, sdLDL, and total cholesterol levels. We think that walnut consumption has a protective effect especially on cardiovascular risk factors.
Keywords: Serum lipids; nutritional habits; small dense low-density lipoprotein; walnut consumption
Amaç: Bu çalışmada, sağlıklı gönüllülerin ceviz tüketiminin serum lipid ve özellikle küçük yoğun düşük yoğunluklu lipoprotein [small dense low-density lipoprotein (sdLDL)] değerleri üzerine etkileri araştırılmıştır. Gereç ve Yöntemler: Bu çalışmaya katılan gönüllülere günde 30 gram ceviz ikram edilmiştir. Cevizler paketler hâlinde kişilere takdim edilmiş ve 21 gün boyunca her sabah kahvaltıda tüketilmesi istenmiştir. Bireylerin ceviz alımını unutmamaları için her gün kısa mesaj hizmeti (SMS) ile hatırlatma yapılmıştır. Bireylerin kan değerleri çalışmanın başında ve sonunda ölçülmüştür. Toplanan kandan serumlar elde edilmiştir ve kan lipid parametreleri rutin olarak biyokimya laboratuvarında analiz edilmiştir. Küçük yoğun LDL parametresi ise ELISA yöntemi ile analiz edilmiştir. Ayrıca kişilerden besin tüketim kaydı formu alınmıştır. Bulgular: Ceviz tüketiminin lipid parametreleri üzerindeki etkisi incelenmiştir. Düzenli ceviz tüketiminin LDL (p<0.001), toplam kolesterol (p<0.001), sdLDL (p<0.001) ve HDL (p=0.008) parametrelerinde anlamlı bir düşüş yarattığı bulunmuştur. Trigliserid seviyelerinde önemli bir değişiklik gözlenmemiştir. Ayrıca karbonhidrat, linoleik asit ve a-linolenik asit alımında Türkiye Beslenme ve Sağlık Araştırmasına göre istatistiksel olarak anlamlı farklılık vardır. Bireylerin enerji, kolesterol, tekli doymamış yağ asidi ve doymuş yağ asidi alımlarında önemli bir fark bulunmamıştır. Sonuç: Bu çalışmada, sağlıklı gönüllülerin diyetine ceviz eklenmesi LDL, sdLDL ve toplam kolesterol seviyelerini düşürmüştür. Ceviz tüketiminin özellikle kardiyovasküler risk faktörleri üzerinde koruyucu bir etkisinin olduğunu düşünüyoruz.
Anahtar Kelimeler: Serum lipidleri; beslenme alışkanlıkları; küçük yoğun düşük yoğunluklu lipoprotein; ceviz tüketimi
- Jenkins DJA, Kendall CWC, Lamarche B, Banach MS, Srichaikul K, Vidgen E, et al. Nuts as a replacement for carbohydrates in the diabetic diet: a reanalysis of a randomised controlled trial. 2018;61(8):1734-47. [Crossref] [PubMed] [PMC]
- Ouchi Y, Sasaki J, Arai H, Yokote K, Harada K, Katayama Y, et al. Ezetimibe lipid-lowering trial on prevention of atherosclerotic cardiovascular disease in 75 or Older (EWTOPIA 75): A randomized, controlled trial. Circulation. 2019;140(12):992-1003. [Crossref] [PubMed]
- Pedersen LR, Olsen RH, Anholm C, Walzem RL, Fenger M, Eugen-Olsen J, et al Weight loss is superior to exercise in improving the atherogenic lipid profile in a sedentary, overweight population with stable coronary artery disease: A randomized trial. Atherosclerosis. 2016;246:221-8. [Crossref] [PubMed]
- Ai M, Otokozawa S, Asztalos BF, Ito Y, Nakajima K, White CC, et al. Small dense LDL cholesterol and coronary heart disease: results from the Framingham Offspring Study. Clin Chem. 2010;56(6):967-76. [Crossref] [PubMed] [PMC]
- Austin MA, Breslow JL, Hennekens CH, Buring JE, Willett WC, Krauss RM. Low-density lipoprotein subclass patterns and risk of myocardial infarction. JAMA. 1988;260(13):1917-21. [Crossref] [PubMed]
- Gerber PA, Nikolic D, Rizzo M. Small, dense LDL: an update. Curr Opin Cardiol. 2017;32(4): 454-9. [Crossref] [PubMed]
- Holt RR, Yim SJ, Shearer GC, Hackman RM, Djurica D, Newman JW, et al. Effects of short-term walnut consumption on human microvascular function and its relationship to plasma epoxide content. J Nutr Biochem. 2015;26(12):1458-66. [Crossref] [PubMed]
- Mohammadi-Sartang M, Bellissimo N, Totosy de Zepetnek JO, Bazyar H, Mahmoodi M, Mazloom Z. Effects of walnuts consumption on vascular endothelial function in humans: A systematic review and meta-analysis of randomized controlled trials. Clin Nutr ESPEN. 2018;28:52-58. [Crossref] [PubMed]
- Hoda P. Prediyabetik metabolik sendromlu erişkin hastalarda beslenme içeriğinin değişmesinin hdl kolesterol üzerine etkisi [Yüksek Lisans Tezi]. Antalya: Akdeniz Üniversitesi Sağlık Bilimler Enstitüsü; 2010. [Link]
- Standards of Medical Care in Diabetes-2017: Summary of Revisions. Diabetes Care. 2017;40(Suppl 1):S4-S5. [Crossref] [PubMed]
- Tuccinardi D, Farr OM, Upadhyay J, Oussaada SM, Klapa MI, Candela M, et al. Mechanisms underlying the cardiometabolic protective effect of walnut consumption in obe se people: A cross-over, randomized, double-blind, controlled inpatient physiology study. Diabetes, Obesity and Metabolism. 2019; 21(9): 2086-95. [Crossref] [PubMed] [PMC]
- Dehne LI, Klemm C, Henseler G, Hermann-Kunz E. The German Food Code and Nutrient Data Base (BLS II.2). Eur J Epidemiol. 1999;15(4):355-9. [Crossref] [PubMed]
- Sağlık Araştırmaları Genel Müdürlüğü, Sağlık Bakanlığı. Türkiye Beslenme ve Sağlık Araştırması 2010: Beslenme Durumu ve Alışkanlıklarının Değerlendirilmesi Sonuç Raporu. T.C. Sağlık Bakanlığı Yayın No: 931. 2014. [Cited: 2020 Oct 4]. Avalaible from: [Link]
- Ros E, Nú-ez I, Pérez-Heras A, Serra M, Gilabert R, Casals E, et al. A walnut diet improves endothelial function in hypercho lesterolemic subjects: a randomized cros sover trial. Circulation. 2004;109(13):1609-14. [Crossref] [PubMed]
- Bamberger C, Rossmeier A, Lechner K, Wu L, Waldmann E, Stark RG, et al. A walnut-enriched diet reduces lipids in healthy caucasian subjects, independent of recommended macronutrient replacement and time point of consumption: A prospective, randomized, controlled trial. Nutrients. 2017;9(10):1097. [Crossref] [PubMed] [PMC]
- Minihane AM, Harland JI. Impact of oil used by the frying industry on population fat intake. Crit Rev Food Sci Nutr. 2007;47(3):287-97. [Crossref] [PubMed]
- Iwamoto M, Imaizumi K, Sato M, Hirooka Y, Sakai K, Takeshita A, et al. Serum lipid profiles in Japanese women and men during consumption of walnuts. Eur J Clin Nutr. 2002; 56(7):629-37. [Crossref] [PubMed]
- Mukuddem-Petersen J, Oosthuizen W, Jerling JC. A systematic review of the effects of nuts on blood lipid profiles in humans. J Nutr. 2005;135(9):2082-9. [Crossref] [PubMed]
- Rock CL, Flatt SW, Barkai HS, Pakiz B, Heath DD. Walnut consumption in a weight reduction intervention: effects on body weight, biological measures, blood pressure and satiety. Nutr J. 2017;16(1):76. [Crossref] [PubMed] [PMC]
- Anderson KJ, Teuber SS, Gobeille A, Cremin P, Waterhouse AL, Steinberg FM. Walnut polyphenolics inhibit in vitro human plasma and LDL oxidation. J Nutr. 2001;131(11):2837-42. [Crossref] [PubMed]
- Liu X, Hill AM, West SG, Gabauer RM, McCrea CE, Fleming JA, et al. Acute peanut consumption alters postprandial lipids and vascular responses in healthy overweight or obese men. J Nutr. 2017;147(5):835-40. [Crossref] [PubMed] [PMC]
- Holscher HD, Guetterman HM, Swanson KS, An R, Matthan NR, Lichtenstein AH, et al. Walnut consumption alters the gastrointestinal microbiota, microbially derived secondary bile acids, and health markers in healthy adults: A randomized controlled trial. J Nutr. 2018;c 148(6):861-7. [Crossref] [PubMed] [PMC]
- Daniels SR, Greer FR; Committee on Nutrition. Lipid screening and cardiovascular health in childhood. Pediatrics. 2008;122(1):198-208. [Crossref] [PubMed]
- Garoufi A, Vorre S, Soldatou A, Tsentidis C, Kossiva L, Drakatos A, et al. Plant sterols-enriched diet decreases small, dense LDL-cholesterol levels in children with hyperc holesterolemia: a prospective study. Italian Journal of Pediatrics. 2014;40:42. [Crossref] [PubMed] [PMC]
- Utarwuthipong T, Komindr S, Pakpeankitvatana V, Songchitsomboon S, Thongmuang N. Small dense low-density lipoprotein concentration and oxidative susceptibility changes after consumption of soybean oil, rice bran oil, palm oil and mixed rice bran/palm oil in hypercholesterolaemic women. J Int Med Res. 2009;37(1):96-104. [Crossref] [PubMed]
- Sabaté J, Cordero-Macintyre Z, Siapco G, Torabian S, Haddad E. Does regular walnut consumption lead to weight gain? Br J Nutr. 2005;94(5):859-64. [Crossref] [PubMed]
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