Turkiye Klinikleri Journal of Medical Sciences

.: ORIGINAL RESEARCH
Romatoid Artrit Hastalarında Serum Orosomukoid-1 (ORM1) Seviyeleri ve ORM1 Gen Promotörünün Metilasyonu Arasındaki İlişkinin İncelenmesi
Investigation of the Relationship Between Serum Orosomucoid-1 (ORM1) Levels and Methylation of ORM1 Gene Promoter in Patients with Rheumatoid Arthritis
Zülfinaz Betül ÇELİKa, Caner GÜNAYDINb, A. Kıvanç CENGİZc, Şengül TURALa
aOndokuz Mayıs Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji ABD, Samsun, TÜRKİYE
bOndokuz Mayıs Üniversitesi Tıp Fakültesi, Tıbbi Farmakoloji ABD, Samsun, TÜRKİYE
cOndokuz Mayıs Üniversitesi Tıp Fakültesi, Romatoloji BD, Samsun, TÜRKİYE
Turkiye Klinikleri J Med Sci.2020;40(4):468-74
doi: 10.5336/medsci.2020-74849
Article Language: TR
Full Text
ÖZET
 Amaç: Romatoid artrit (RA), patogenezinde genetik ve epigenetik faktörlerin etkili olduğu kronik inflamatuar bir hastalıktır. Orosomukoid-1 (ORM1), antiinflamatuar özelliğe sahip olan bir akut faz yanıt proteinidir ve yapılan çalışmalarda RA patogenezi ile ilişkili olduğu gösterilmiştir. Ancak hastalıktaki rolü tam olarak aydınlatılmamıştır. Bu çalışmada, RA hastalarında ve sağlıklı kontrollerde serum ORM1 düzeylerinin ve ORM1 gen promotörü metilasyonunun araştırılması amaçlandı. Gereç ve Yöntemler: Klinik olarak RA teşhisi olan 40 hasta ve 31 sağlıklı kontrol çalışmaya dâhil edildi. Tüm donörlere ait periferik kan örneklerindeki serum ORM1 seviyeleri ELISA yöntemi ile belirlendi. Aynı bireyler için kandan DNA izolasyonu yapıldı. Elde edilen DNA örneklerine bisülfit modifikasyonu yapıldı. ORM1 gen promotörü için tasarlanan metilasyon spesifik primerler kullanılarak, ORM1 geni promotör metilasyon profilleri, metilasyon spesifik polimeraz zincir reaksiyonu metodu ile belirlendi. Bulgular: Serum ORM1 seviyesinin, kontrol grubunda hasta grubuna kıyasla yaklaşık 3,2 kat yüksek olduğu gözlendi (p

Anahtar Kelimeler: Arthritis, rheumatoid; orosomucoid; DNA methylation
ABSTRACT
 Objective: Rheumatoid arthritis (RA) is a chronic inflammatory disease, and genetic and environmental factors are wellknown causes. Orosomucoid-1 (ORM1) is an acute phase response protein with anti-inflammatory properties and associated with the RA pathogenesis. However, its role in RA has not been fully known. In this study, we aimed to investigate serum ORM1 levels and methylation of ORM1 gene promoter in RA patients and healthy controls. Material and Methods: The study group consisted of 40 RA patients and 31 healthy controls. Serum levels of ORM1 in RA patients and healthy controls were determined by using the ELISA method. After genomic DNA was isolated from blood samples, DNA samples were modified with bisulfite. Promoter methylation of ORM1 was analyzed by methylation-specific PCR using methylation-specific primers designed for the ORM1 promoter region. Results: The serum levels of ORM1 in control group were found approximately 3.2 times higher compared to the patient group (p

Keywords: Artrit, romatoid; orosomukoid; DNA metilasyonu
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