Amaç: Bu çalışmanın amacı, retrograd kavite preparasyonunda farklı şelasyon ajanlarının kalsiyum silikat esaslı materyallerle bağlanma dayanımına etkisinin push-out testi ile ölçülmesidir. Gereç ve Yöntemler: Çalışmamızda 84 adet tek kanallı maksiller santral diş kullanıldı. Kök kanal tedavisinden sonra ultrasonik uçlarla retrograd kavite preparasyonu yapıldı. Örnekler şelasyon ajanlara göre 3 gruba (n=28) ayrıldı: %17 EDTA ve %0,2 kitosan ve kontrol grubunda %0,9 salin kullanıldı. Daha sonra her gruptan alınan örnekler kalsiyum silikat esaslı dolgu malzemelerine göre 2 gruba (n=14) ayrıldı; MTA Angelus ve MTA Flow. Her numunenin apikal kısmından 2 mm kalınlığında kesitler alındı ve push-out testinde incelendi. Sonuçlar, Mann-Whitney U ve Student t-testleri kullanılarak istatistiksel olarak analiz edildi. Kırılma tiplerini karşılaştırmak için ki-kare testi kullanıldı. p<0,05 istatistiksel olarak anlamlı kabul edildi. Bulgular: Bu in vitro çalışma sonucunda, 2 farklı şelasyon ajanı kontrol grubu serum fizyolojik ile karşılaştırıldığında aralarında istatistiksel olarak anlamlı bir fark bulunmuştur (p<0,001) %0,2 kitosan ve %17 EDTA grupları arasında istatistiksel olarak anlamlı bir fark görülmemiştir (p>0,05). MTA Angelus ile MTA Flow dolgu materyalleri arasında fark saptanmıştır (p<0,001). MTA Flow dolgu materyali daha yüksek bağlanma değerlerine sahip olduğu görülmüştür. Sonuç: Retrograd kavite preparasyonunda şelasyon ajanı kullanımı bağlanma dayanımını olumlu yönde etkilemektedir ve başarı oranı güncel preparasyon teknikleri ve biyouyumlu malzemelerin kullanılması ile artırılabilir.
Anahtar Kelimeler: Kitosan; EDTA; ultrasonikler
Objective: The aim of this study to evaluate the effect of different chelating agents on the bond strength of calcium silicate-based materials in retrograde cavity preparation. Material and Methods: In our study, 84 maxillary central teeth with a single root canal were used. After root canal treatment, retrograde cavity preparation was performed with ultrasonic tips. The samples were randomly divided into 3 groups (n=28) based on the chelating agents: 17% EDTA and 0.2% chitosan and 0.9% saline was used in the control group. Then the samples from each group were subdivided into 2 groups (n=14) based on calcium silicate based filling materials; MTA Angelus and MTA Flow. 2 mm thick sections were taken from the apical part of each sample and examined in the push-out bond strength test. Data were statistically analyzed using Mann-Whitney U and Student t-tests. The chi-square test was used to compare the failure mode. p<0.05 was considered statistically significant. Results: When two different chelating agents were compared with the control group, chelating agents showed significantly higher bond strength values (p<0.001). There was no significant difference between the 0.2% chitosan and 17% EDTA groups (p>0.05). There was a difference between MTA Angelus and MTA Flow filling materials (p<0.001). MTA Flow filling material showed higher bond strength values. Conclusion: The use of chelating agents in retrograde cavity preparation has a positive effect on the bond strength and the success rate can be increased by using current preparation techniques and biocompatible materials.
Keywords: Chitosan; EDTA; ultrasonics
- Deplazes P, Peters O, Barbakow F. Comparing apical preparations of root canals shaped by nickel-titanium rotary instruments and nickel-titanium hand instruments. J Endod. 2001;27(3):196-202. [Crossref] [PubMed]
- von Arx T. Apical surgery: A review of current techniques and outcome. Saudi Dent J. 2011;23(1):9-15. [Crossref] [PubMed] [PMC]
- Ingle JI, Simon JH, Machtou P, Bogaerts P. Outcome of endodontic treatment and re-treatment. J Endod. 2002;5:747-68. [Link]
- Akncbay H, Senel S, Ay ZY. Application of chitosan gel in the treatment of chronic periodontitis. J Biomed Mater Res B Appl Biomater. 2007;80(2):290-6. [Crossref] [PubMed]
- Silva PV, Guedes DF, Nakadi FV, Pécora JD, Cruz-Filho AM. Chitosan: a new solution for removal of smear layer after root canal instrumentation. Int Endod J. 2013;46(4):332-8. [Crossref] [PubMed]
- Dutta PK, Dutta J, Tripathi V. Chitin and chitosan: chemistry, properties and applications. J Sci Ind Res. 2004;63:20-31. [Link]
- Guimarães BM, Vivan RR, Piazza B, Alcalde MP, Bramante CM, Duarte MAH. Chemical-physical properties and apatite-forming ability of mineral trioxide aggregate flow. J Endod. 2017;43(10):1692-6. [Crossref] [PubMed]
- Pelepenko LE, Saavedra F, Antunes TBM, Bombarda GF, Gomes BPFA, Zaia AA, et al. Physicochemical, antimicrobial, and biological properties of White-MTAFlow. Clin Oral Investig. 2021;25(2):663-72. [Crossref] [PubMed]
- Pane ES, Palamara JE, Messer HH. Critical evaluation of the push-out test for root canal filling materials. J Endod. 2013;39(5):669-73. [Crossref] [PubMed]
- Neelakantan P, Ahmed HMA, Wong MCM, Matinlinna JP, Cheung GSP. Effect of root canal irrigation protocols on the dislocation resistance of mineral trioxide aggregate-based materials: A systematic review of laboratory studies. Int Endod J. 2018;51(8):847-61. [Crossref] [PubMed]
- von Arx T, Jensen SS, Hänni S, Friedman S. Five-year longitudinal assessment of the prognosis of apical microsurgery. J Endod. 2012;38(5):570-9. [Crossref] [PubMed]
- Song M, Chung W, Lee SJ, Kim E. Long-term outcome of the cases classified as successes based on short-term follow-up in endodontic microsurgery. J Endod. 2012;38(9):1192-6. [Crossref] [PubMed]
- Kim S, Kratchman S. Modern endodontic surgery concepts and practice: a review. J Endod. 2006;32(7):601-23. [Crossref] [PubMed]
- Bernardes RA, de Moraes IG, Garcia RB, Bernardineli N, Baldi JV, Victorino FR, et al. Evaluation of apical cavity preparation with a new type of ultrasonic diamond tip. J Endod. 2007;33(4):484-7. [Crossref] [PubMed]
- Khabbaz MG, Kerezoudis NP, Aroni E, Tsatsas V. Evaluation of different methods for the root-end cavity preparation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;98(2):237-42. [Crossref] [PubMed]
- Ishikawa H, Sawada N, Kobayashi C, Suda H. Evaluation of root-end cavity preparation using ultrasonic retrotips. Int Endod J. 2003;36(9):586-90. [Crossref] [PubMed]
- Rosales-Leal JI, Olmedo-Gaya V, Vallecillo-Capilla M, Luna-del Castillo JD. Influence of cavity preparation technique (rotary vs. ultrasonic) on microleakage and marginal fit of six end-root filling materials. Med Oral Patol Oral Cir Bucal. 2011;16(2):e185-9. [Crossref] [PubMed]
- Violich DR, Chandler NP. The smear layer in endodontics - a review. Int Endod J. 2010;43(1):2-15. [Crossref] [PubMed]
- Neelakantan P, Nandagopal M, Shemesh H, Wesselınk P. The effect of root dentin conditioning protocols on the push-out bond strength of three calcium silicate sealers. Int J Adhes Adhes. 2015;60:104-8. [Crossref]
- Ulusoy Öİ, Görgül G. Effects of different irrigation solutions on root dentine microhardness, smear layer removal and erosion. Aust Endod J. 2013;39(2):66-72. [Crossref] [PubMed]
- Kamble AB, Abraham S, Kakde DD, Shashidhar C, Mehta DL. Scanning electron microscopic evaluation of efficacy of 17% ethylenediaminetetraacetic acid and chitosan for smear layer removal with ultrasonics: an in vitro study. Contemp Clin Dent. 2017;8(4):621-6. [Crossref] [PubMed] [PMC]
- Del Carpio-Perochena A, Bramante CM, Duarte MA, de Moura MR, Aouada FA, Kishen A. Chelating and antibacterial properties of chitosan nanoparticles on dentin. Restor Dent Endod. 2015;40(3):195-201. [Crossref] [PubMed] [PMC]
- Penumaka R, Konagala RK, Shaik J, Ram Sunil CH, Reddy PL, Kiran Naik MK. Scanning electron microscopy evaluation of chitosan and carboxymethyl chitosan as retrograde smear layer removing agents. J Conserv Dent. 2019;22(6):573-7. [Crossref] [PubMed] [PMC]
- Shah DK, Sanap-Tandale A, Aggarwal S, Sengar E, Borkar A, Shetty S. Comparative evaluation of the sealing ability of filling materials on root end cavities treated with smear layer removing agents: A confocal laser scanning microscopic study. J Conserv Dent. 2019;22(5):495-9. [Crossref] [PubMed] [PMC]
- Agarwal S, Raghu R, Shetty A, Gautham PM, Souparnika DP. An in vitro comparative evaluation of the effect of three endodontic chelating agents (17% ethylenediamine tetraacetic acid, 1% peracetic acid, 0.2% chitosan) on the push out bond strength of gutta percha with a new bioceramic sealer (BioRoot RCS). J Conserv Dent. 2019;22(5):475-8. [Crossref] [PubMed] [PMC]
- Benz K, Markovic L, Jordan RA, Novacek C, Jackowski J. In vitro evaluation of the apical seal of two different retrograde filling materials. Aust Endod J. 2017;43(1):29-33. [Crossref] [PubMed]
- Jung J, Kim S, Kim E, Shin SJ. Volume of voids in retrograde filling: comparison between calcium silicate cement alone and combined with a calcium silicate-based sealer. J Endod. 2020;46(1):97-102. [Crossref] [PubMed]
- Gokturk H, Ozkocak I. The effect of different chelators on the dislodgement resistance of MTA Repair HP, MTA Angelus, and MTA Flow. Odontology. 2022;110(1):20-6. [Crossref] [PubMed]
- Silva EJ, Carvalho NK, Zanon M, Senna PM, DE-Deus G, Zuolo ML, et al. Push-out bond strength of MTA HP, a new high-plasticity calcium silicate-based cement. Braz Oral Res. 2016;30(1):S1806-83242016000100269. [Crossref] [PubMed]
- Govindaraju L, Neelakantan P, Gutmann JL. Effect of root canal irrigating solutions on the compressive strength of tricalcium silicate cements. Clin Oral Investig. 2017;21(2):567-71. [Crossref] [PubMed]
- Guneser MB, Akbulut MB, Eldeniz AU. Effect of various endodontic irrigants on the push-out bond strength of biodentine and conventional root perforation repair materials. J Endod. 2013;39(3):380-4. [Crossref] [PubMed]
.: Process List