Turkiye Klinikleri Journal of Veterinary Sciences

.: ORIGINAL RESEARCH
Prevalence of Cryptosporidium spp. in Diarrheic Dogs in Van Province
Van Bölgesindeki İshalli Köpeklerde Bulunan Cryptosporidium spp.'nin Yaygınlığı
Adnan AYANa, Özlem ORUNÇ KILINÇb
aDepartment of Genetics, Van Yüzüncü Yıl University Faculty of Veterinary Medicine, Van, TURKEY
bÖzalp Vocational School, Van Yüzüncü Yıl University, Van, TURKEY
Turkiye Klinikleri J Vet Sci. 2020;11(2):64-8
doi: 10.5336/vetsci.2020-77151
Article Language: EN
Full Text
ABSTRACT
 Objective: Cryptosporidiosis is a zoonotic apicomplexan protozoon that causes significant gastrointestinal problems in humans and animals alike. In the present study, we investigated the prevalence of Cryptosporidium spp. in diarrheic dogs in Van province by using two different methods. Material and Methods: A total of 153 fecal samples were collected from diarrheic stray dogs aging up to 4 months between 2018-2019 at Animal Care and Rehabilitation Center of Van Metropolitan Municipality in Van province. Samples were stained with with Kinyoun's acid-fast method and examined at x100 magnification under the microscope. DNA were extracted from all the 153 samples using RTA stool DNA isolation kit and Nested PCR was carried out using suitable primers. Results: Microscopic examination revealed the presence of Cryptosporidium spp. oocysts in 81 out of 153 (52.94%) samples whereas Cryptosporidium spp. specific 826-864 bp size bands were obtained in 99 (64.7%) of 153 samples using Nested PCR. Conclusion: The prevalence of Cryptosporidiosis in dogs up to 4 months of age is quite high that suggests further research on the species of Cryptosporidium in dogs.

Keywords: Cryptosporidium spp.; dog; nested PCR; Turkey; Van province
ÖZET
 Amaç: Cryptosporidiosis, insan ve hayvanlarda önemli gastrointestinal sorunlara sebep olabilen apikompleksan bir protozoon tarafından oluşturulan zoonoz bir hastalıktır. Bu çalışmada, Van bölgesindeki ishalli köpeklerde Cryptosporidium spp.'nin prevalansının iki farklı yöntem kullanılarak araştırılması amaçlanmıştır. Gereç ve Yöntemler: Çalışmanın materyalini 2018-2019 yılları arasında Van İlindeki Van Büyükşehir Belediyesi Hayvan Bakımevi ve Rehabilitasyon Merkezindeki 4 aylığa kadar olan 153 adet ishalli sokak köpek dışkısı oluşturmaktadır. Dışkı örnekleri Kinyoun'un acid-fast metodu ile boyanarak mikroskop altında x100'lük büyütmede incelendi. 153 örneğin tamamından RTA dışkı DNA izolasyon kiti kullanılarak DNA ekstraksiyonu yapıldı. Ardından ilgili primerlerle Nested PCR yapıldı. Bulgular: Mikroskobik incelemede 153 örneğin 81'inde %52,94 Cryptosporidium spp. ookistlerine rastlanıldı. Nested PCR sonucuna göre örneklerin 99'unda (%64,7) Cryptosporidium spp. için spesifik 826-864 bp büyüklüğünde bantlar elde edildi. Sonuç: Van ilindeki 4 aylığa kadar olan ishalli köpeklerdeki Cryptosporidiosis prevalansının oldukça yüksek olduğu ve köpeklerdeki Cryptosporidium türleri hakkında daha fazla araştırma yapılması gerektiği kanısına varılmıştır.

Anahtar Kelimeler: Cryptosporidium spp; köpek; nested PCR; Türkiye; Van ili
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