Turkiye Klinikleri Journal of Veterinary Sciences

.: ORIGINAL RESEARCH
Keçilerde Metan Gazı Salınımının Tespitinde Laktuloz Nefes Testinin Yeri: Kesitsel Araştırma
The Role of Lactulose Breath Test in Detection of Methane Gas Release in Goats: A Cross-Sectional Study
Deniz ALIÇ URALa
aAydın Adnan Menderes Üniversitesi Veteriner Fakültesi, Fakülte Çiftliği, Aydın, Türkiye
Turkiye Klinikleri J Vet Sci. 2024;15(2):54-60
doi: 10.5336/vetsci.2024-105058
Article Language: TR
Full Text
ÖZET
 Amaç: Rasyon bileşimi, ırk ve çevresel faktörler (sıcaklık, stres, uygun olmayan barındırma vb.) rumen fonksiyonunu ve enterik metan (CH4) emisyonunu (salınımı) olumsuz etkileyen yaygın faktörlerdir. Her bir geviş getiren hayvanın her gün küresel iklim değişikliği ile birlikte en güçlü sera gazlarından olan CH4 salınımına neden olduğu göz önünde bulundurulursa keçilerin buradaki katkısı da muhakkak araştırılmalıdır. Gereç ve Yöntemler: Kesitsel (durum saptama) bu araştırmada, saha koşullarında sağlıklı görünümde 29 Saanen Keçisinde onar g laktuloz yükleme testi sonrası nefes testi yapılarak CH4 gazı salınımı araştırıldı. Özel torbalara 12-47 sn'lik sürelerde laktuloz yüklemesini müteakip 0, 30, 60 ve 90. dk'larda ekshale nefes (hava) toplanarak eş zamanlı uygulamalar sonrası elde edilen gaz dinamiklerinin araştırılması için Sunvou Nefes Analizatörüne aktarıldı. Elde edilen gaz dinamiklerine ait grafikler software program aracığında PDF dokümanlarına dönüştürülerek sonuçlar irdelendi. Bulgular: Grupların kendi içerisinde karşılaştırmasında fark çıkmasa da ölçüm zamanlarının hepsi farklı çıkmıştır. Laktuloz yüklemesi sonrası sırası ile 0, 30, 60 ve 90. dk'lar karşılaştırıldığında sabah 08.00 verileri ile öğlen 12.00 değerleri arasında belirgin istatistiksel farklılıklar belirendi. Sıfırıncı dk yani bazal çıkış değerleri (laktuloz yükleme testi öncesi ortalama±standart hata değerleri ppm cinsiden) 12,20±0,92 (sabah 08.00) ve 24,13±4,67 (öğlen 12.00) (p<0,001) metan emisyonunu gösterirken, 90. dk'larda sırası ile sabah ve öğlen değerleri 14,20±3,33 ve 22,25±3,54 ppm olarak belirlendi. Sonuç: Sıcaklık artışı ve dolaylı stresin metan gazı salınımını artırdığı ve keçilerde bu durumun dikkate alınarak gerekli kontrol stratejilerinin geliştirilmesi gerekliliği açıktır.

Anahtar Kelimeler: İklim; keçi; metan gazı; sıcaklık stresi
ABSTRACT
 Objective: Diet composition, breed and environmental factors (temperature, stress, inappropriate housing etc.) are common factors that negatively affect rumen function and enteric methane (CH4) emission. Considering that each ruminant animal causes CH4 emission, one of the most powerful greenhouse gases together with global climate change, every day, the contribution of goats here should definitely be investigated. Material and Methods: In this cross-sectional (condition determination) study, CH4 gas emission was investigated by performing breath tests after 10 g lactulose loading test in 29 healthy Saanen Goats under field conditions. Exhaled breath (air) was collected in special bags at 0th, 30th, 60th and 90th minutes following lactulose loading for 12-47 seconds and transferred to Sunvou Breath Analyzer for the investigation of gas dynamics obtained after simultaneous applications. The obtained gas dynamics graphs were converted into PDF documents by software program and the results were examined. Results: Although there was no difference in the comparison of the groups within themselves, all measurement times were different. When the 0th, 30th, 60th and 90th minutes after lactulose loading were compared, significant statistical differences were determined between the 08.00 morning data and 12.00 noon values. While the 0th minute, i.e. basal output values (mean±standard error values before lactulose loading test in ppm) showed methane emissions of 12.20±0.92 (08.00 morning) and 24.13±4.67 (12.00 noon) (p<0.001), at the 90th minute, the morning and noon values were determined as 14.20±3.33 and 22.25±3.54 ppm, respectively. Conclusion: It is clear that temperature increase and indirect stress increase methane gas release and that the necessary control strategies should be developed in goats by taking this situation into account.

Keywords: Climate; goat; methane gas; heat stress
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