Turkiye Klinikleri Journal of Ophthalmology

.: ORIGINAL RESEARCH
Is There Any Association Between the Retinal Vein Occlusion and the Thiol-Disulfifide Homeostasis Which is an Oxidative Stress Indicator?
Retinal Ven Tıkanıklığı ve Bir Oksidatif Stres Belirteci Olan Tiyol-Disülfit Dengesi Arasında Herhangi Bir İlişki Var mıdır?
Osman Ahmet POLATa, Ali KURTa, Raşit KILIÇa, Rukiye NARb, Özkan KOCAMIŞa
Departments of,
aOphthalmology,
bBiochemistry Ahi Evran University Faculty of Medicine, Kırşehir, TURKEY
Turkiye Klinikleri J Ophthalmol. 2019;28(1):23-8
doi: 10.5336/ophthal.2018-60159
Article Language: EN
Full Text
ABSTRACT
 Objective: In this study, it is aimed to investigate the dynamic thiol-disulfide homeostasis which is an indicator of oxidative stress in retinal vein occlusion patients. Material and Methods: Thirty nine retinal vein occlusion patients and 38 age-gender matched control subjects were included in the study. Exclusion criteria for retinal vein occlusion group were: ocular history and symptoms exceeding 3 weeks, any other history of ocular disease except the retinal vein occlusion, any previous ocular surgeries or lasers, any previous treatment or intra-vitreal injection for the retinal vein occlusion, any other systemic disease except the arterial hypertension. The control group included patients without any ocular or systemic diseases. Native thiol, total thiol, disulfide levels were studied in the serum samples of all participants and native/total thiol and disulfide/total thiol ratios were calculated as percentage. Results: Total thiol, native thiol, disulfide, native/total thiol and disulfide/total thiol ratio were not significantly different between the retinal vein occlusion and the control groups. In the control group, age was inversely correlated with the total thiol level, but not with the other serum parameters. In the retinal vein occlusion group, there was no correlation among the age and the serum parameters but the body mass index (BMI) was inversely correlated with the total thiol level. Conclusion: There was not any significant difference between the retinal vein occlusion patients and the control subjects for the thiol-disulfide homeostasis.

Keywords: Retinal vein occlusion; thiol-disulfide homeostasis; thiol; disulfide
ÖZET
 Amaç: Bu çalışmada retinal ven tıkanıklığı ile bir oksidatif stres belirteci olan dinamik tiyol-disülfit dengesi arasındaki ilişkinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Otuz dokuz retinal ven tıkanıklığı hastası ve 38 yaş ve cinsiyet eşleştirilmiş kontrol birey olmak üzere toplam 77 katılımcı çalışmaya alınmıştır. Retinal ven tıkanıklığı grubu için çalışmadan dışlanma kriterleri: 3 haftayı aşan göz şikayet ve bulguları, retinal ven tıkanıklığı dışında başka oküler hastalık öyküsünün olması, daha önce oküler cerrahi ve lazer öyküsünün olması, retinal ven tıkanıklığı için daha önce intravitreal enjeksiyon dahil olmak üzere herhangi bir tedavi uygulanmış olması, arteryel hipertansiyon dışında sistemik hastalık öyküsünün olması olarak belirlenmiştir. Kontrol grubu sistemik ve oküler hastalığı olmayan katılımcılardı. Native tiyol, total thiol, disülfit düzeyleri katılımcıların kan serumu örneklerinde çalışılarak belirlenmiştir ve native/total tiyol ve disülfit/total tiyol oranları hesaplanarak yüzde olarak kaydedilmiştir. Bulgular: Total tiyol, native tiyol, disülfit, native/total tiyol oranı ve disülfit/total tiyol oranları retinal ven tıkanıklığı ve kontrol grubu arasında karşılaştırıldığında anlamlı fark bulunmamıştır. Kontrol grubunda yaş ve total tiyol seviyeleri arasında negatif korelasyon olduğu, fakat diğer parametreler arasında anlamlı ilişki olmadığı bulunmuştur. Retinal ven tıkanıklığı grubunda yaş ve serum parametreleri arasında anlamlı ilişki bulunmamış, fakat beden kitle indeksi (BKİ) ile total tiyol düzeyleri arasında negatif korelasyon bulunmuştur. Sonuç: Retinal ven tıkanıklığı ve kontrol grupları arasında tiyol-disülfit dengesi açısından anlamlı fark bulunmamıştır.

Anahtar Kelimeler: Retinal ven tıkanıklığı; disülfit; tiyol; tiyol-disülfit dengesi
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