Amaç: Bu in vitro çalışmanın amacı, başlangıç mine çürüğü olan dişler üzerinde florür, kazein fosfopeptid amorf kalsiyum fosfat (CPPACP), novamin ile lazer uygulaması ve ozon uygulamasının tek başına veya birlikte kullanımının, remineralizasyon etkinliğini değerlendirmektir. Gereç ve Yöntemler: Bu amaçla sığır dişleri toplanarak, başlangıç çürüğü elde edilmiştir. Her grupta 15 diş olacak şekilde 11 grup (florür, CPP-ACP, novamin, lazer, lazer+florür, lazer+CPP-ACP, lazer+novamin, ozon, ozon+florür, ozon+CPP-ACP, ozon+novamin) oluşturulmuştur. Tedavi etkinliği mikrosertlik ölçümü, taramalı elektron mikroskobu (SEM) görüntüleri ve enerji dağılımlı X-ışını spektroskopisi (EDX) mineral analizi yapılarak değerlendirilmiştir. Elde edilen verilerin istatistiksel analizi, tek yönlü ve 3 yönlü varyans analizi kullanılarak yapılmıştır (p<0,05). Bulgular: Mikrosertlik verileri karşılaştırıldığında en yüksek değer lazer+florür grubunda izlenirken, en düşük ozon grubunda gözlenmiştir. EDX analiziyle kalsiyum (Ca) ve fosfat (P) oranları değerlendirildiğinde en yüksek Ca değeri lazer+novamin grubunda izlenirken, en yüksek P değeri novamin, lazer+CPP-ACP, lazer+novamin ve ozon+novamin gruplarında bulunmuştur. SEM değerlendirmesi sonucunda, tüm grupların mine yüzeyleri sağlam ve demineralize örneğe göre morfolojik değişimler göstermiştir. Sonuç: Çalışmamızın sonuçlarına göre Er:YAG lazer ve kombine remineralize edici ajan uygulaması, başlangıç çürüğünün remineralizasyonunda etkili bir metot olarak bulunmuştur. Ozon uygulamasının başlangıç çürüğünün remineralizasyonundaki etkinliği ileri çalışmalarla araştırılmalıdır.
Anahtar Kelimeler: Demineralizasyon; mine, lazer; mine; ozon; remineralizasyon
Objective: This in vitro study aimed to evaluate effectiveness of remineralization alone or combined use of fluoride, casein phosphopeptide amorphous calcium phosphate (CPP-ACP), novamin, laser and ozone application on enamel initial caries. Material and Methods: For this purpose, bovine teeth were collected and obtained initial caries lesions. 11 groups (fluoride, CPP-ACP, novamin, laser, laser+fluoride, laser+CPP-ACP, laser+novamin, ozone, ozone+fluoride, ozone+CPP-ACP, ozone+novamin) each group to be 15 teeth were established. Effectiveness of treatment was evaluated by micro-hardness measurement, scanning electron microscopy (SEM) images and energy dispersive X-ray spectroscopy (EDX). Statistical analysis of the resulting data was performed (p<0.05). Results: When compared microhardness data, the laser+fluoride group observed the highest value and the ozone group observed the lowest group. When compared the EDX analysis of calcium (Ca) and phosphate (P) ratio, the highest Ca value was observed in the laser+novamin group, the highest P value was observed in the novamin group, the laser+CPP-ACP group, the laser+novamin group and the ozone+novamin group. As a result of SEM evaluation, enamel surfaces of the entire groups showed morphological changes based on sound and demineralized sample. Conclusion: According to our results, combined Er:YAG laser and remineralizating agents application was found to be an effective method for the remineralization of initial caries. Remineralization effectiveness of the application of ozone in the initial caries should be investigated in the future studies.
Keywords: Demineralization; enamel; laser; ozone; remineralization
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