Turkiye Klinikleri Journal of Medical Sciences

.: ORIGINAL RESEARCH
Evaluation of Diagnosis Season and in Situ Hybridization EBER Results in Hodgkin Lymphoma: A Single Center Retrospective Study
Hodgkin Lenfomada Tanı Mevsimi ve in Situ Hibridizasyon EBER Sonuçlarının Değerlendirilmesi: Tek Merkezli Retrospektif Çalışma
Damla Çağla PATIRa , Özlem LİMONCUb , Derya DEMİRc , Nazan ÖZSANc , Mine HEKİMGİLc , Ajda GÜNEŞa , Nur SOYERa , Fahri ŞAHİNa , Filiz VURALa , Güray SAYDAMa , Mahmut TÖBÜa
aEge University Faculty of Medicine, Department of Hematology, İzmir, Türkiye
bEge University Faculty of Medicine, Department of Internal Medicine, İzmir, Türkiye
cEge University Faculty of Medicine, Department of Pathology, İzmir, Türkiye
Turkiye Klinikleri J Med Sci. 2025;45(3):158-65
doi: 10.5336/medsci.2024-107137
Article Language: EN
Full Text
ABSTRACT
 Objective: Hodgkin lymphoma (HL) is a hematological malignancy with a generally favorable prognosis, yet its pathogenesis remains unclear, and unknown risk factors affecting incidence and mortality are still being explored. This study aimed to assess the influence of diagnosis season and the presence of in situ hybridization (ISH) Epstein-Barr encoded RNA (EBER) on HL survival. Material and Methods: We evaluated 232 HL patients treated at Ege University Faculty of Medicine Hematology Clinic from January 1, 1994, to December 31, 2023. Results: Among the 232 patients (41.4% female and 58.6% male), the mean age at diagnosis was 38.42 years (SD: 15.99), with a median age of 35. The most common subtype was nodular sclerosing (47%), followed by mixed cellularity (29.3%) and nodular lymphocyte predominant (NLP) (9.9%). EBER was detected in 45.7% of patients, while 54.3% were EBER negative. The highest diagnosis rate occurred in winter (30.6%), and the lowest in spring (19.8%). EBER presence did not vary by season, and no significant difference in overall survival times was found between EBER positive and negative patients (p=0.427). Conclusion: Our findings indicate that EBER positivity does not significantly impact the prognosis of HL, with no seasonal variability in EBER rates. The lack of significant survival differences suggests that EBER has limited relevance in HL management. These insights contribute to the understanding of HL's clinical practice and pathogenesis, highlighting areas for future research.

Keywords: Hodgkin disease; Epstein-Barr virus encoded RNA; seasons
ÖZET
 Amaç: Hodgkin lenfoma (HL), en iyi prognoza sahip hematolojik malignitelerden biridir. Bununla birlikte, HL'nin patogenezi tam olarak anlaşılmamış olup, HL insidansı ve mortalitesini etkileyebilecek potansiyel bilinmeyen risk faktörleri araştırılmaya devam etmektedir. Bu çalışmanın amacı, tanı mevsiminin ve in situ hibritizasyon (ISH) Epstein-Barr kodlu RNA (EBER) varlığının HL üzerindeki etkisini değerlendirmektir. Gereç ve Yöntemler: Bu çalışma, 1 Ocak 1994 ile 31 Aralık 2023 tarihleri arasında Ege Üniversitesi Tıp Fakültesi Hematoloji Kliniği'nde HL tanısı almış ve tedavi edilmiş 232 hastayı içermektedir. Bulgular: Bu çalışmada toplam 232 hasta (%41,4 kadın ve %58,6 erkek) değerlendirilmiştir. Tanı anındaki ortalama yaş 38,42 (SS: 15,99) olarak belirlenmiş, medyan yaş ise 35 bulunmuştur. HL'nin en yaygın alt tipi 47% ile nodüler sklerozan, ardından %29,3 ile karışık hücreli ve %9,9 ile nodüler lenfosit baskın (NLP) olarak saptanmıştır. EBER, hastaların %54,3'ünde yokken, %45,7'sinde tespit edilmiştir. En yüksek tanı oranı kış mevsiminde %30,6 olarak gözlemlenirken, en düşük oran bahar mevsiminde %19,8 olarak belirlenmiştir. EBER varlığı mevsimlere göre değişiklik göstermemiş ve EBER negatif ile pozitif hastalar arasında ortalama genel sağkalım sürelerinde istatistiksel olarak anlamlı bir fark bulunmamıştır (p=0,427). Sonuç: Çalışmamız, EBER pozitifliğinin Hodgkin Lenfoma (HL) prognozu üzerinde önemli bir etkisi olmadığını ortaya koymuştur. EBER pozitiflik oranları mevsimsel değişkenlik göstermemektedir. Bu bulgular, HL'nin klinik pratiği ve patogenezi hakkında önemli bilgiler sunmakta ve gelecekteki araştırmalar için yeni yönlerin belirlenmesine katkıda bulunmaktadır.

Anahtar Kelimeler: Hodgkin hastalığı; Epstein-Barr virus kodlanmış RNA; mevsimler
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