Turkiye Klinikleri Journal of Veterinary Sciences

.: ORIGINAL RESEARCH
Effects of Silymarin on Blood and Tissue Parameters in Sodium Fluoride-Induced Hepatotoxicity and Oxidative Stress in Rats
Ratlarda Sodyum Florid ile Oluşturulan Hepatotoksite ve Oksidatif Streste Silimarin'in Kan ve Doku Parametreleri Üzerine Etkileri
Hilal KARAGÜLa, Mert PEKCANa, Efe KURTDEDEa
aDepartment of Biochemistry, Ankara University Faculty of Medicine, Ankara, TURKEY
Turkiye Klinikleri J Vet Sci. 2020;11(2):53-9
doi: 10.5336/vetsci.2020-76255
Article Language: EN
Full Text
ABSTRACT
 Objective: To investigate the protective and curative effect of silymarin against fluoride-induced hepatotoxity and oxidative degradation in rats. Material and Methods: Of the 45 rats, 9 were given only sodium fluoride (F); 9 were given only silymarin (S), 9 were given only tap water (K), 9 were given sodium fluoride and silymarin together (FS) and 9 were given silymarin after sodium fluoride was completed (T). Results: In Group F, serum AST level was significantly higher than in control group (p<0.001) and in Group FS and group T, serum AST and ALT levels were lower than in Group F, which was evaluated as improved toxic effect of sodium fluoride by silymarin. When compared with the control group; in Group F, a decrease in serum SOD and GSH-Px values and an increase in MDA levels showed oxidative deterioration in liver tissue; high SOD and GSHPx levels and low MDA levels in Group S and Group T showed that silymarin prevents and improves tissue damage.Histopathological significant differences were found between Group F, Group S and Group T in terms of deterioration in remak cords (p<0.01 p=0,007). In addition, there was a significant difference between Group F, Group T and Group FS in terms of degenerative changes (p<0.001). Conclusion: It was concluded that sodium fluoride has toxic and destructive effects on liver tissue and silymarin has a protective and healing effect against these effects.

Keywords: Liver function tests; silymarin; sodium fluoride
ÖZET
 Amaç: Silimarinin sıçanlarda florür kaynaklı hepatotoksite ve oksidatif bozunmaya karşı koruyucu ve iyileştirici etkisini araştırmak amaçlandı.Gereç ve Yöntemler: Çalışmada kullanılan 45 ratın, 9'una sadece NaF (F); 9'una sadece silimarin (S), 9'una sadece çeşme suyu (K), 9'una NaF ve silimarin birlikte (FS) ve 9'una, NaF verilmesi tamamlandıktan sonra, silimarin (T) verildi. Bulgular: Grup F'de, serum AST düzeyinin kontrol grubuna göre önemli düzeyde yüksek (p<0,001) ve Grup FS'de ve Grup T'de, serum AST ve ALT düzeylerinin F grubuna göre düşük olması bulguları", sodyum floridin karaciğer dokusuna olan toksik etkisi ve buna karşı silimarinin koruyucu ve iyileştirici etkisi olarak değerlendirildi. Bu sonucun histopatolojik olarakta desteklendiği görüldü. Kontrol grubuyla yapılan karşılaştırmalarda; karaciğer dokusunda Grup F'de, SOD ve GSH-Px değerlerindeki düşüş ve MDA düzeyindeki artış karaciğer dokusundaki oksidatif yıkımı gösterirken; Grup FS'de ve Grup T'de SOD ve GSH-Px düzeylerinin yüksek, MDA düzeylerinin düşük bulunması, silimarinin oksidatif doku yıkımını önlediği ve bunu iyileştirdiğinin göstergesi oldu. Histopatolojik olarak remakerlerde bozulma açısından Grup F, Grup S ve Grup T arasında anlamlı fark bulundu (p<0,01 p=0,007). Ayrıca, Grup F, Grup T ve Grup FS arasında dejeneratif değişiklikler açısından anlamlı fark vardı. Sonuç: Bu çalışmada; sodyum floridin karaciğer dokusuna toksik ve yıkılmayıcı etki yaptığı; bu etkilere karşı silimarin'in koruyucu ve iyileştirici etkisinin olduğu sonucuna varıldı.

Anahtar Kelimeler: Karaciğer fonksiyon testleri; silymarin; sodyum florid
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