Turkiye Klinikleri Journal of Medical Sciences

.: ORIGINAL RESEARCH
Effects of Hypoxic Preconditioning on Oxidant-Antioxidant Systems in Rat Lung
Sıçanlarda Hipoksik Ön Koşullamanın Akciğerdeki Oksidan-Antioksidan Sistemler Üzerine Etkisi
Sevtap KILINÇa, Sevin GÜNEYa
aDepartment of Physiology, Gazi University Faculty of Medicine, Ankara, TURKEY
Turkiye Klinikleri J Med Sci. 2019;39(2):194-201
doi: 10.5336/medsci.2018-62968
Article Language: EN
Full Text
ABSTRACT
 Objective: Hypoxic preconditioning allows cells to gain resistance to hypoxic damage. There are a limited number of studies suggesting that hypoxic preconditioning increases antioxidant capacity in the lung. In this study, we aimed to evaluate effects of hypoxic preconditioning on oxidant/ antioxidant systems in rat lung. Material and Methods: Rats were divided into 4 groups: control, preconditioning (PC) (10% O2), severe hypoxia (SH) (7% O2) and PC + SH. The parameters related to oxidative stress and antioxidant defense mechanisms, which are malondialdehyde (MDA) levels, total oxidant system (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI), superoxide dismutase (SOD) and glutathione (GSH) activity, were measured. Results were evaluated using the One-Way ANOVA and t-test; p<0.05 was considered significant. Results: Compared with the control group, MDA levels decreased in all hypoxic groups; decrements in PC and SH groups were statistically significant. Compared with the control group, levels of TOS showed a significant increase in the PC+SH group. OSI of PC+SH group was significantly higher than other groups. There was no significant difference in TAC levels between the groups. Compared to other groups, SOD activity showed a significant decrease in the PC+SH group. GSH levels showed a significant decrease in PC+SH group compared to both control and PC group. Conclusion: Our findings suggest that hypoxic preconditioning does not have an effect on antioxidant defense systems in lungs, but severe hypoxia does affect oxidant/antioxidant systems.

Keywords: Hypoxia; hypoxic preconditioning; lung; reactive oxygen species; oxidant/antioxidant systems
ÖZET
 Amaç:Hipoksik önkoşullama, hücrelerin hipoksik hasara karşı direnç kazanmasını sağlar. Hipoksik önkoşullamanın akciğerdeki antioksidan kapasiteyi arttırdığı yönündeki çalışmalar sınırlı sayıdadır. Bu çalışmada sıçan akciğerinde hipoksik ön koşullamanın oksidan/antioksidan sistemler üzerindeki etkilerinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: Sıçanlar; kontrol (K), ön koşullama (ÖK) (%10 O2), ağır hipoksi (AH) (%7 O2) ve ağır hipoksi+ön koşullama (AH+ÖK) olmak üzere 4 gruba ayrılmıştır. Dokularda oksidatif stres ve antioksidan savunma mekanizmaları ile ilişkili parametreler olan malondialdehit (MDA) düzeyi, total oksidan sistem (TOS), total antioksidan kapasite (TAK), oksidatif stres indeksi (OSİ), süperoksit dismutaz (SOD) and glutatyon (GSH) aktiviteleri ölçülmüştür. Sonuçlar Tek Yönlü ANOVA ve t testi kullanılarak değerlendirildi; p<0,05 değerleri anlamlı kabul edildi. Bulgular: Kontrol grubu ile karşılaştırıldığında, MDA seviyeleri tüm hipoksik gruplarda azalma göstermiştir; ÖK ve AH gruplarındaki bu azalma istatistiksel olarak anlamlıdır. Kontrol grubu ile karşılaştırıldığında, TOS düzeyleri AH+ÖK grubunda belirgin bir artış göstermiştir. AH+ÖK grubunun OSİ değerleri diğer gruplara göre anlamlı düzeyde yükselmiştir. Gruplar arasında TAK düzeyleri bakımından anlamlı bir fark bulunamamıştır. Diğer gruplara kıyasla, SOD etkinliği ağır hipoksi+ön koşullama grubunda önemli bir azalma göstermiştir. Kontrol ve ÖK grubuyla kıyaslandığında GSH düzeyi AH+ÖK grubunda anlamlı bir azalma göstermiştir. Sonuç: Bulgularımız hipoksik ön koşullamanın akciğerlerde antioksidan savunma sistemlerin üzerinde etkili olmadığını ancak ağır hipoksinin oksidan/antioksidan sistemleri etkilediğini göstermektedir.

Anahtar Kelimeler: Hipoksi; hipoksik ön koşullama; akciğer; reaktif oksijen türleri; oksidan/antioksidan sistemler
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