Journal of Traditional Medical Complementary Therapies

.: ORIGINAL RESEARCH
Effect of Propolis in Dogs with Transmissible Venereal Tumor Treated Vincristine Sulphate: Experimental Study
Vinkristin Sülfat ile Tedavi Edilen Bulaşıcı Zührevi Tümörlü Köpeklerde Propolisin Etkisi: Deneysel Çalışma
Hikmet Aysın USTAa , Hasan Hüseyin ORUÇb , Onur KIZILGÜNc , Ender UZABACId , Musa Özgür ÖZYİĞİTc
aBursa Uludağ University Institute of Health Science, Department of Pharmacology and Toxicology, Bursa, Türkiye
bBursa Uludağ University Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Bursa, Türkiye
cBursa Uludağ University Faculty of Veterinary Medicine, Department of Pathology, Bursa, Türkiye
dBursa Uludağ University Faculty of Veterinary Medicine, Department of Biometrics, Bursa, Türkiye
J Tradit Complem Med. 2024;7(2):151-61
doi: 10.5336/jtracom.2023-100442
Article Language: EN
Full Text
ABSTRACT
 Objective: The objective of the study was to determine mean of treatment period, some liver enzyme levels and blood parameters in dogs with TVT that were administered standard vincristine sulphate and administered propolis by different routes. Material and Methods: A total of 24 dogs with TVT (9 males, 15 females), irrespective of breed and age, were included in the study. The study was divided into four groups: a control group (n=6, receiving vincristine sulphate), an oral group (n=7, receiving vincristine sulphate + 100 mg/kg raw propolis daily), a local group (n=5, receiving vincristine sulphate + 200 mg/ml raw propolis daily), and an oral+local group (n=6, receiving vincristine sulphate + 100 mg/kg raw propolis daily, orally and 200 mg/ml raw propolis daily, locally). Weekly clinical examinations were conducted, and smears and blood samples were collected. Staining and histopathological examination were performed using the Diff-quick staining test. Results: Although there are not significant differences, mean treatment weeks in all groups that added propolis were lower than control group. Neutropenia, neutrophilia, leukopenia, and leukocytosis were confirmed most of the treated dogs. Alanine transaminase, aspartate transaminaz, alkaline phosphatase, gamma glutamyltransferase and lactate dehydrogenase levels were in the reference values in control and experimental groups. Conclusion: The levels of liver enzymes in all groups were within the reference range, indicating that vincristine sulphate did not cause hepatotoxicity. TVT treatment periods in groups used propolis were shorter than the standard vincristine sulphate therapy. Antitumoral effects of propolis should much more extensively study with animal and clinical experiments.

Keywords: Transmissible venereal tumor; vincristine; propolis
ÖZET
 Amaç: Bu çalışmada, vinkristin sülfat ile standart medikal tedavi alan TVT'li köpeklerde, farklı yollarla propolis uygulamasının, iyileşme sürelerine, bazı karaciğer enzim düzeyleri ve kan parametrelerine etkisinin incelenmesi amaçlandı. Gereç ve Yöntemler: Çalışmaya 24 TVT'li köpek (9 erkek, 15 dişi), ırk ve yaş gözetmeksizin dahil edildi. Çalışma, kontrol grubu (n=6, sadece vinkristin sülfat), oral grup (n=7, vinkristin sülfat+ ham propolis 100 mg/kg, günde bir), lokal grup (n=5, vinkristin sülfat+ ham propolis 200 mg/ml, günde bir) ve oral+lokal grup (n=6, vinkristin sülfat + ham propolis 100 mg/kg, günde bir, oral+ham propolis 200 mg/ml günde bir, lokal) olarak dört gruba ayrılarak yapıldı. Her hafta, klinik kontrolden sonra smear ve kan alındı. Diff-quick boyama testi ile boyama ve histopatolojik inceleme yapıldı. Bulgular: İstatistiki olarak fark bulunmamakla birlikte propolis ilave edilen tüm gruplarda iyileşme süresinin daha kısa olduğu görüldü. Nötropeni, nötrofili ile lökopeni ve lökositoz tedavi edilen köpeklerin büyük çoğunluğunda tespit edildi. Alanin aminotransferaz, aspartat aminotransferaz, alkalen fosfataz, gama glutamil transferaz ve laktat dehidrojenaz düzeylerinin tüm gruplarda referans değer aralığında olduğu görüldü. Sonuç: Kontrol ve deney grubu köpeklerin karaciğer enzim düzeyleri referans değer aralığında olduğu görüldü ve vinkristin sülfatın hepatotoksisiteye neden olmadığı kanısına varıldı. TVT'nin standart vinkristin sülfat ile tedavisinde propolis ilave edilen gruplarda iyileşme daha hızlı olmuştur. Propolisin antitümoral etkisi daha detaylı olarak hayvan ve klinik çalışmalarla yapılmalıdır.

Anahtar Kelimeler: Bulaşıcı zührevi tümör; vinkristin; propolis
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