Turkiye Klinikleri Journal of Medical Sciences

.: ORIGINAL RESEARCH
Effect of Different Doses of Vitamin D3 on Anxiety in Rats Without Vitamin D Deficiency: An Experimental Study
D Vitamini Eksikliği Olmayan Sıçanlarda Farklı Dozlarda D3 Vitamininin Anksiyete Üzerine Etkisi: Deneysel Çalışma
Nazan DOLUa,d, Haifa MASUDb,d, Zahour Gamal Eddn ASMAEILc,d
aİstanbul Medipol University Faculty of Medicine, Department of Physiology, İstanbul, Türkiye
bÇankırı Karatekin University Faculty of Chemistry, Department of Chemistry, Çankırı, Türkiye
cBenghazi University Faculty of Science, Department of Zoology, Benghazi, Libya
dBaşkent University Faculty of Medicine, Department of Physiology. Ankara, Türkiye
Turkiye Klinikleri J Med Sci. 2025;45(1):17-23
doi: 10.5336/medsci.2024-104917
Article Language: EN
Full Text
ABSTRACT
 Objective: The effects of vitamin D3 on anxiety are controversial. In this study, the effects of two different doses of vitamin D3 on anxiety in rats without vitamin D3 deficiency were investigated by sympathetic skin response (SSR), elevated plus maze (EPM), and open field (OF) tests. SSR measures eccrine sweat gland activity innervated with sympathetic fibers. Increased skin conductance level (SCL, a parameter of SSR), decreased open arm duration at EPM and spent time in the peripheral area at OF show anxiety status. Material and Methods: The present study was conducted with 36 Wistar male rats (aged 6-8 weeks, 200-250 g). Normal water (Sham group, Group 1), 400IU vitamin D3 (Group 2), and 1000IU vitamin D3 (Group 3) were given to rats per day with oral gavage. After 8 weeks, tonic SCL (resting), phasic SCL (with auditory stimulus), and behavioral tests were measured. Results: The Tonic SCL of Group 2 and Group 3 were higher than the sham group. There was no significant difference between the tonic SCLs of Group 2 and Group 3. The phasic SCL of Group 2 was higher than Group 1. The time spent in the center at OF in Group 2 and Group 3 was significantly lower than in Group 1. Group 3 spent less time on the open arms of the EPM. Conclusion: Administration of different doses of vitamin D to rats without vitamin D3 deficiency increased anxiety. This situation should be considered when giving/taking vitamin D3 supplements.

Keywords: Calcitriol; rats; open field test; elevated plus maze; galvanic skin response
ÖZET
 Amaç: D3 vitamininin anksiyete üzerine etkileri tartışmalıdır. Bu çalışmada, D3 vitamini eksikliği olmayan sıçanlara iki farklı dozda D3 vitamini uygulamasının anksiyeteye etkileri, sempatik deri cevabı (SDC), yüksek artı labirent (YAL) ve açık alan (AA) testleri ile araştırıldı. SDC, sempatik liflerle innerve edilen ekrin ter bezi aktivitesini ölçer. Deri iletkenlik seviyesinin (DİS, SDC'nın bir parametresi) artması, YAL'de açık kol süresinin azalması ve AA'da periferik alanda geçirilen sürenin artması anksiyete durumunu gösterir. Gereç ve Yöntemler: Bu çalışma 36 Wistar erkek sıçanda (6-8 haftalık, 200-250 g) gerçekleştirildi. Sıçanlara günde bir kez normal su (Sham grubu, Grup 1), 400 IU D3 vitamini (Grup 2) ve 1000 IU D3 vitamini (Grup 3) oral gavajla verildi. 8 hafta sonra tonik DİS (dinlenme), fazik DİS (işitsel uyaranla) ve davranış testleri ölçüldü. Bulgular: Grup 2 ve Grup 3'ün tonik DİS'si sham grubuna göre daha yüksekti. Grup 2 ve 3 arasında tonik DİS açısından anlamlı bir fark yoktu. Grup 2'nin fazik DİS'si Grup 1'e göre daha yüksekti. Grup 2 ve Grup 3'te AA'da merkezde geçirilen süre Grup 1'e göre anlamlı derecede düşüktü. Grup 3, YAL'in açık kollarında daha az zaman harcadı. Sonuç: D3 vitamini eksikliği olmayan sıçanlara farklı dozlarda D vitamini uygulanması anksiyeteyi artırdı. D3 vitamini takviyesi verirken/alırken bu durum dikkate alınmalıdır.

Anahtar Kelimeler: Kalsitriol; sıçanlar; açık alan testi; yükseltilmiş artı labirent; galvanik deri yanıtı
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