Turkiye Klinikleri Journal of Veterinary Sciences

.: ORIGINAL RESEARCH
Effect of Cinnamon on Distribution and Number of Mast Cells in Diabetic Rat Liver
Diyabetik Sıçan Karaciğerinde Tarçının Mast Hücrelerinin Dağılımı ve Sayısı Üzerine Etkisi
Tuğrul ERTUĞRULa, Nazife ÜLKER ERTUĞRULb
aDepartment of Histology and Embryology, Ondokuz Mayıs University, Faculty of Veterinary Medicine, Samsun, Türkiye
bDepartment of Physiology, Fırat University Faculty of Medicine, Elazığ, Türkiye
Turkiye Klinikleri J Vet Sci. 2022;13(2):51-7
doi: 10.5336/vetsci.2022-92082
Article Language: EN
Full Text
ABSTRACT
 Objective: In the present study, we investigated the effects of cinnamon on distribution and number of mast cells (MCs) in liver tissue of diabetic rats. Material and Methods: The rats used as the control group received no application. All rats in the diabetes and diabetes+cinnamon groups were made diabetic by a single dose intraperitoneal injection of streptozotocin. In our study, after establishment of diabetes, cinnamon extract was administered daily by oral gavage for 14 days to both cinnamon and diabetes+cinnamon groups. To determine the MCs, the tissue sections were stained both with toluidin blue (TB) and Alcian blue/Safranin O (AB/SO) methods. Results: It was determined that the number of MCs in the portal areas increased with diabetes. As a notable finding, it was observed in our study that cinnamon reduced the MC numerical increase that increases with diabetes in the diabetes+cinnamon group. As a result of the AB/SO combined staining method application, it was shown that there were 3 types of MC populations as blue AB(+), red SO(+) and mixed color AB/SO(+) in rat liver tissue. It was found that cinnamon application was effective on the SO(+) content in diabetes. Conclusion: In conclusion, the findings of this study show that diabetes alters the number of MC and cinnamon can reduced the increase in diabetesrelated MC numbers. In addition, this study demonstrated the possible effects of diabetes and cinnamon on the histochemical and morphological differences of MCs in rat liver.

Keywords: Diabetes; cinnamon; mast cell; histochemistry
ÖZET
 Amaç: Bu çalışmada, tarçının diyabetik sıçanların karaciğer dokusundaki mast hücrelerinin (MH) dağılımı ve sayısı üzerindeki etkileri araştırıldı. Gereç ve Yöntemler: Kontrol grubu olarak kullanılan ratlara herhangi bir uygulama yapılmadı. Diyabet ve diyabet+tarçın gruplarındaki tüm ratlar tek doz intraperitoneal streptozotosin enjeksiyonu ile diyabetik hâle getirildi. Çalışmamızda, diyabet oluşturulduktan sonra tarçın ve diyabet+tarçın gruplarındaki tüm ratlara 14 gün boyunca günlük olarak oral gavaj yoluyla tarçın uygulandı. Mast hücrelerini belirlemek için doku kesitleri hem toluidin blue (TB) hem de Alcian blue/Safranin O (AB/SO) yöntemleri ile boyandı. Bulgular: Diyabetle birlikte portal alanlardaki MH sayısının arttığı belirlendi. Dikkate değer bir bulgu olarak çalışmamızda, tarçının diyabet+tarçın grubunda diyabetle birlikte artan MH sayısal artışını azalttığı gözlendi. AB/SO kombine boyama metodu uygulaması sonucunda, rat karaciğer dokusunda mavi AB(+), kırmızı SO(+) ve karışık renkli AB/SO(+) olmak üzere 3 tip mast hücre popülasyonunun olduğu saptandı. Diyabette tarçın uygulamasının, SO(+) içeriği üzerinde etkili olduğu bulundu. Sonuç: Sonuç olarak bu çalışmanın bulguları, diyabetin MH sayısını değiştirdiği ve tarçının diyabete bağlı MH sayılarındaki artışı engelleyebileceğini göstermektedir. Ayrıca bu çalışma, sıçan karaciğerindeki MH'lerin histokimyasal ve morfolojik farklılıkları üzerinde diyabet ve tarçının olası etkilerini göstermiştir.

Anahtar Kelimeler: Diyabet; tarçın; mast hücre; histokimya
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