Turkiye Klinikleri Journal of Dental Sciences

.: ORIGINAL RESEARCH
Effect of Caffeic Acid Phenethyl Ester on Gingival Tissue Inflammation in Experimental Periodontitis: Experimental Study
Deneysel Periodontitiste Kafeik Asit Fenetil Esterin Diş Eti Doku İnflamasyonuna Etkisi: Deneysel Çalışma
Esra DEMİRa, Feyza OTAN ÖZDENb, Bahattin AVCIc
aDepartment of Periodontology, Bezmiâlem Vakıf University Faculty of Dentistry, İstanbul, Türkiye
bDepartment of Periodontology, Ondokuz Mayıs University Faculty of Dentistry, Samsun, Türkiye
cDepartment of Biochemistry, Ondokuz Mayıs University Faculty of Medicine, Samsun, Türkiye
Turkiye Klinikleri J Dental Sci. 2022;28(4):883-9
doi: 10.5336/dentalsci.2022-93042
Article Language: EN
Full Text
ABSTRACT
 Objective: This study aimed to investigate the anti-inflammatory effect of two different dosages of caffeic acid phenethyl ester (CAPE) on lipopolysaccharide-induced experimental periodontitis (EP). Material and Methods: Forty Sprague Dawley rats were randomly divided into four groups: control, EP, EP treated with 5 μmol/kg/day of CAPE (EP+CAPE 5), and EP treated with 10 μmol/kg/day of CAPE (EP+CAPE 10). Followed by the EP, CAPE was administered intraperitoneally to the EP+CAPE groups for 28 days. Samples were investigated biochemically using an enzyme-linked immunosorbent assay kit and alveolar bone loss was measured morphometrically. Results: In both of the CAPE groups, the levels of interleukin-1 beta and tumor necrosis factor alpha (TNF-α) in the gingiva were significantly lower than those in the EP group (p<0.001). The decrease in tissue levels of TNF-α was greater in the EP+CAPE 10 group than in the EP+CAPE 5 group in a dose-dependent manner. Serum analysis of the cytokines showed no significant difference between the groups. Conclusion: Within the limits of this study, CAPE showed an anti-inflammatory effect and is claimed to be a novel agent in improving the results of periodontal therapy. CAPE may be valuable as an alternative host modulating agent for the treatment of periodontal disease.

Keywords: Caffeic acid phenethyl ester; periodontal diseases; interleukin-1 beta; tumor necrosis factor-alpha
ÖZET
 Amaç: Bu çalışma, 2 farklı dozda kafeik asit fenetil esterin (KAFE) lipopolisakkarit ile indüklenen deneysel periodontitis (DP) üzerindeki antiinflamatuar etkisini araştırmayı amaçlamıştır. Gereç ve Yöntemler: Kırk Sprague Dawley sıçanı rastgele 4 gruba ayrılmıştır: kontrol, DP, 5 μmol/kg/gün KAFE ile tedavi edilen DP (DP+KAFE 5) ve 10 μmol/kg/gün KAFE ile tedavi edilen DP (DP+KAFE 10). DP'yi takiben DP+KAFE gruplarına 28 gün süreyle intraperitoneal olarak KAFE uygulanmıştır. Numuneler, enzim bağlı immünosorbent tahlil kiti kullanılarak biyokimyasal olarak araştırılmış ve alveolar kemik kaybı morfometrik olarak ölçülmüştür. Bulgular: Her iki KAFE grubunda da diş eti interlökin-1 beta ve tümör nekroz faktörü-alfa (TNF-α) seviyeleri DP grubuna göre anlamlı derecede düşük bulunmuştur (p<0,001). TNF-α'nın doku seviyelerindeki düşüş, doza bağlı bir şekilde DP+KAFE 5 grubuna göre DP+KAFE 10 grubunda daha fazla olmuştur. Sitokinlerin serum analizi, gruplar arasında anlamlı bir farklılık göstermemiştir. Sonuç: Bu çalışmanın sınırları dâhilinde, KAFE'nin antiinflamatuar etkisi gösterilmiş ve periodontal tedavinin sonuçlarını arttırmada etkili bir ajan olabileceği ileri sürülmüştür. KAFE, periodontal hastalığın tedavisi için alternatif bir konak modüle edici ajan olarak değerli olabilir.

Anahtar Kelimeler: Kafeik asit fenetil ester; periodontal hastalıklar; interlökin-1 beta; tümör nekroz faktörü-alfa
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