Turkiye Klinikleri Journal of Veterinary Sciences

.: ORIGINAL RESEARCH
Doğal Peptid Analoğu Laktoferrisin B'nin Kaşıntılı Köpeklerde Antipruritik Amaçla Kullanımı ve Epidermal Hidrasyon ile pH Üzerine Etkileri
The Use of Natural Peptide Analogue Lactoferrin B for Antipruritic Purposes in Pruritic Dogs and its Effects on pH with Epidermal Hydration
Kerem URALa, Buğrahan Bekir YAĞCIb, Hasan ERDOĞANa, Serdar PAŞAa, Mehmet GÜLTEKİNa, Gizem GÜLa, Halil İbrahim ADAKa, Elif TÜRKa
aİç Hastalıkları ABD, Aydın Adnan Menderes Üniversitesi Veteriner Fakültesi, Aydın, TÜRKİYE
bİç Hastalıkları ABD, Kırıkkale Üniversitesi Veteriner Fakültesi, Kırıkkale, TÜRKİYE
Turkiye Klinikleri J Vet Sci. 2019;10(1):11-20
doi: 10.5336/vetsci.2019-70049
Article Language: TR
Full Text
ÖZET
 Amaç: Dermatolojik rahatsızlıkların yarattıkları görsel kusurun yanı sıra hayvan sağlığı üzerine olumsuz etkileri göz önünde bulundurulursa, alerjik dermatitli köpeklerde en sık karşılaşılan bulgu olan kaşıntının giderilmesi önem arz etmektedir. Kronikleşmeye yüz tutan alerjik dermatitli köpeklerde kaşıntı ile beraber epidermal pH ve hidrasyonun bozulması (epidermal koruyucu bariyerin ortadan kalkması) komplikasyonlara neden olmaktadır. Hasta sahipleri en az köpekleri kadar kaşıntıdan ve lezyonların doğal görünümünden rahatsız olmaktadırlar. Etiyolojik açıdan değerlendirildiğinde kaşıntı; dermatolojik, nörolojik, psikojenik, sistemik, karışık veyahutta bilinmeyen sebeplere göre sınıflandırılmaktadır. Bu çalışmada, doğal peptid analoğu laktoferrisin B'nin kaşıntılı köpeklerde antipuritik amaçla kullanımı ve epidermal hidrasyon ile pH üzerine etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Olgularımızda kaşıntının skorlanması için geliştirilmiş (adapte edilmiş) gözlemsel analog ölçek (cetvel) kullanıldı. Bulgular ve Sonuç: Callegari Soft Plus korneometre analizi kullanılarak gerçekleştirilen bu çalışmada, karşılaştırmalı değerlendirildiğinde, kaşıntı skoruna ilişkin ortanca (aralık) değerleri sağaltım öncesinde 8 (5-10), tedavi sonrasında ise 2 (0-4) olarak belirlendi (p<0,001). Diğer yandan (ortalama±standart sapma) sağaltım öncesi ve sonrası epidermal pH (4,4±0,4 vs. 5,4±0,2; p˂0,001) ile hidrasyon değerleri (21,3±11,6 94,0±9,5; p˂0,001) açısından belirgin istatistiksel farklılıklar saptanması laktoferrisin B'nin etkinliği ile ilişkilendirilebilinir. Sonuç olarak, doğal peptid analoğu laktoferrisin B'nin kaşıntılı köpeklerde antipuritik etkinliğinin yüksek olduğu ve epidermal hidrasyon ile pH üzerine de tamir edici, düzenleyici (böylelikle epidermal bariyerin fonksiyonel bütünlüğünü sağlayıcı ve düzenleyici) etkiler sağlayabileceği rahatlıkla öne sürülebilir.

Anahtar Kelimeler: Laktoferrisin b; pH; hidrasyon; deri
ABSTRACT
 Objective: In view of the negative effects on animal health beyond the cosmetic defect caused by dermatological diseases, it is important to remove the most common finding in dogs with allergic dermatitis. Epidermal pH and degradation of the hydration (removal of the epidermal protective barrier) cause complications in dogs with chronic allergic dermatitis. Patient owners suffer from at least as small dogs as itching and natural appearance of the lesions. From an etiological point of view, itching might be classsified in dermatological, neurological, psychogenic, systemic, mixed or unknown reasons. In this study, the effects of natural peptide lactoferricin on antiparitic purposes and epidermal hydration were investigated. Material and Methods: In our cases, an observational analog scale (ruler) which was adapted for scoring the itch was used. Results and Conclusion: The median (range) values of the pruritus score were determined as 8 (5-10) before treatment and 2 (0-4) after treatment when compared with Callegari Soft Plus corneometer analysis (p<0.001). On the other hand (mean±standard deviation) epidermal pH before and after treatment (4,4±0.4 vs. 5.4±0.2; p<0.001) and hydration values (21.3±11.6 94.0±9.5; Significant statistical differences in p<0.001) can be correlated with the efficacy of lactoferricin. As a result, it can easily be argued that natural peptid analogue lactoferricin can have antipuritic activity in pruritic dogs and can provide repairing and regulating effects on the epidermal hydration and pH (thus providing the functional integrity of the epidermal barrier).

Keywords: Lactoferricin b; pH; hydration; skin
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