Turkiye Klinikleri Journal of Medical Sciences

.: ORIGINAL RESEARCH
Does Metformin Protect Against Brain Injury After Subarachnoid Hemorrhage? An Experimental Study
Subaraknoid Kanama Sonrası Metformin Erken Beyin Hasarını Önleyebilir mi? Deneysel Çalışma
Mahmut ÇAMLAR a, Çağlar TÜRK b, Fatma Demet ARSLANc, Meryem Merve ÖREN d , Emrah AKÇAYe , Gülden DİNİZ f , Füsun ÖZER a
aDepartment of Neurosurgery, University of Health Sciences Tepecik Training and Research Hospital, İzmir, TURKEY
bDepartment of Neurosurgery, Artvin State Hospital, Artvin, TURKEY
cDepartment of Biochemistry, University of Health Sciences Tepecik Training and Research Hospital, İzmir, TURKEY
dDepartment of Public Health, İstanbul University İstanbul Faculty of Medicine, İstanbul, TURKEY
eDepartment of Neurosurgery, University of Health Sciences Bozyaka Training and Research Hospital, İzmir, TURKEY
fDepartment of Pathology, İzmir Demokrasi University Faculty of Medicine, İzmir, TURKEY
Turkiye Klinikleri J Med Sci.2020;40(4):393-8
doi: 10.5336/medsci.2020-76488
Article Language: EN
Full Text
ABSTRACT
 Objective: Hyperglycemia is one of the main risk factors for early brain injury and an outcome in patients with subarachnoid hemorrhage (SAH). Agents that regulate blood glucose levels can prevent the development of early brain injury. The present experimental study aimed to investigate the histological and anti-apoptotic effects of metformin on SAH in a rat model. Material and Methods: An SAH model was applied in 28 male Wistar rats who were randomly grouped as follows: control (Group 1), SAH alone (Group 2), SAH with hyperglycemia (Group 3), and metformin-treated SAH with hyperglycemia (Group 4). Intraperitoneal streptozotocin (50 mg/kg) was administered for 7 d to induce hyperglycemia before conducting the experiment. All rats were sacrified 72 hours after SAH. Histopathological and immunohistochemical evaluations were also conducted. Results: The thickness of the basilar artery wall was significantly different among the four groups (p=0.003). The thickness of the basilar artery wall was higher in Group 3 than in Groups 1 and 2. Blood glucose values were significantly different among the four groups (p

Keywords: Early brain injury; hyperglycemia; metformin; subarachnoid hemorrhage
ÖZET
 Amaç: Subaraknoid kanama (SAK) sonrası gelişen hiperglisemi, erken beyin hasarı ve sağkalım için ana risk faktörlerinden biridir. Kan glukoz seviyesini düzenleyen ajanlar erken beyin hasarını önleyebilirler. Bu deneysel çalışma ratlarda SAK modelinde metforminin histolojik ve anti-apoptotik etkilerini etkilerini ortaya koymayı amaçlamıştır. Gereç ve Yöntemler: Toplam 28 adet Wistar albino cinsi rata deneysel SAK modeli uygulanarak rastgele 4 gruba ayrıldı: kontrol (Grup1), sadece SAK uygulananlar (Grup 2), SAK ve hiperglisemi geliştirilenler (Grup 3), SAK ve hiperglisemi uygulanıp metformin verilenler (Grup 4). Deney öncesi ratlarda hiperglisemi intraperitoneal streptozosin (50 mg/kg) ile sağlandı. SAK sonrası 72. saatte ratlar sakrifiye edildi. Histopatolojik ve immünohistokimyasal incelemeler yapıldı. Bulgular: Dört grupta baziller arter kalınlıklarının anlamlı farklı olarak saptandı (p=0,003). Grup 3'te baziller arter duvar kalınlıkları grup 1 ve 2'den daha fazla bulundu. Kan glukoz seviye değerleri dört grup arasında farklı olarak saptandı (p

Anahtar Kelimeler: Erken beyin hasarı; hiperglisemi; metformin; subaraknoid kanama
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