Turkiye Klinikleri Cardiovascular Sciences

.: ORIGINAL RESEARCH
Circulating Long Intergenic Non-Coding RNA LINC01538 as Potential Novel Biomarker for Acute Myocardial Infarction: Prospective Cohort Study
Akut Myokard Enfarktüsü için Potansiyel Yeni Bir Biyobelirteç Olarak Dolaşan Uzun İntergenik Kodlamayan RNA LINC01538: Prospektif Kohort Çalışma
Christina SOLIMANa, Sara AGWAb, Shadia FATHYc, Manal EMAMc, Ahmed ELSHAZLYd, Doaa IBRAHIMe
aAssistant Lecturer of Biochemistry, Faculty of Science, Ain Shams University, Cairo 11382, Egypt
bProfessor of Clinical Pathology and Molecular Genomics, Medical Ain Shams Research Institute (MASRI), Faculty of Medicine, Ain Shams University, Cairo, Egypt
cProfessor of Biochemistry, Faculty of Science, Ain Shams University, Cairo 11382, Egypt
dLecturer of Cardiology, Ain Shams University, Cairo, Egypt
eAssistant Professor of Biochemistry, Faculty of Science, Ain Shams University, Cairo 11382, Egypt
Turkiye Klinikleri J Cardiovasc Sci. 2022;34(2):55-61
doi: 10.5336/cardiosci.2022-90615
Article Language: EN
Full Text
ABSTRACT
 Objective: Non-coding RNAs are the RNAs with no protein coding function, long non-coding RNAs (LncRNAs) are the non-coding RNAs with more than 200 nucleotides. During the journey of discovering novel biomarkers for diagnosis of myocardial infarction (MI), the principle of studying the LncRNA as a potential would be always attractive, as it needs easy sample collection, has high detection sensitivity and high myocardial tissue specificity. Our main objective was to investigate the potential of LINC01538 as a novel diagnostic biomarker for MI. Material and Methods: Quantitative real-time polymerase chain reaction (RT-qPCR) was used to assess the expression of the serum LINC01538 in 50 ST Elevation MI (STEMI) patients and 48 controls. Results: The study showed a significant increase in serum level expression of LINC01538 in MI patients compared to controls [12 (6.6- 21.8) vs. 0.07 (0.01-0.2), p<0.001]. LINC01538 expression level in MI group was positively correlated with creatine kinase MB and high sensitive cardiac troponin I (hs-cTnI) (r=0.39, p=0.006 and r=0.22, p=0.007, respectively). Hs-cTnI found to have diagnostic value for MI with an area under curve (AUC) 0.917 [95% confidence interval (CI): 0.855- 0.979, p<0.001] at an optimal cutoff point of 1.45 ng/L, 90% sensitivity and 91% specificity. However, LINC01538 showed the highest diagnostic value with an AUC 0.980 (95% CI: 0.942- 1, p<0.001) at an optimal cut-off point of 1.76, 100% sensitivity and 98% specificity. Conclusion: Our findings have, for the first time, demonstrated that circulating LINC01538 are highly expressed in patients with MI, functioning as potential novel biomarker for diagnosis.

Keywords: LncRNA; LINC01538; novel biomarker; myocardial infarction
ÖZET
 Amaç: Kodlamayan RNA'lar protein kodlama işlevi olmayan RNA'lardır, uzun kodlamayan RNA'lar (LncRNA'lar), 200'den fazla nükleotid içeren kodlamayan RNA'lardır. Miyokard enfarktüsünün (MI) tanısı için yeni biyobelirteçleri keşfetme yolculuğu sırasında, LncRNA'yı bir potansiyel olarak inceleme ilkesi, kolay örnek toplamaya ihtiyaç duyduğundan, yüksek algılama duyarlılığına ve yüksek miyokardiyal dolarak LINC01538'in potansiyelini araştırmaktır. Gereç ve Yöntemler: Elli ST yükselmeli MI (STEMI) hoku özgüllüğüne sahip olduğundan her zaman çekici olacaktır. Asıl amacımız MI için yeni bir biyobelirteç astası ve 48 kontrolde serum LINC01538 ekspresyonunu değerlendirmek için kantitatif gerçek zamanlı polimeraz zincir reaksiyonu (RT-qPCR) kullanıldı. Bulgular: MI hastalarında kontrollere kıyasla serum LINC01538 düzeylerinde anlamlı artış olduğu görüldü [12 (6.6-21.8) vs. 0.07 (0.01-0.2), p<0.001]. MI grubunda LINC01538 ekspresyon düzeyi kreatin kinaz MB ve yüksek sensitif kardiyak troponin I (hs-cTnI) ile pozitif olarak körele idi [sırasıyla, 12 (6.6-21.8) vs. 0.07 (0.01-0.2), p<0.001]. Hs-cTnI, eğri altında kalan alan (AUC) 0,917 [%95 güven aralığı (GA): 0,855-0,979, p<0,001] ile 1,45 ng/L optimal kesme noktasında, %90 duyarlılık ve %91 özgüllük ile MI için tanısal değere sahip bulundu. Bununla birlikte, LINC01538, 1,76 optimal kesme noktasında, %100 duyarlılık ve %98 özgüllükte AUC 0.980 (%95 CI: 0.942-1, p<0.001) ile en yüksek tanı değerini göstermiştir. Sonuç: Bulgularımız, ilk kez, dolaşımdaki LINC01538'in MI hastalarında yüksek oranda eksprese edildiğini ve tanı için potansiyel yeni bir biyobelirteç olarak işlev gördüğünü göstermiştir. Bununla birlikte, potansiyel uygulamayı doğrulamak ve tutarlılığı belirlemek için ST elevasyonsuz akut koroner sendromda test edilmesi gerekir.

Anahtar Kelimeler: LncRNA; LINC01538; yeni biyobelirteç; myokard enfarktüsü
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