Turkiye Klinikleri Journal of Dental Sciences

.: ORIGINAL RESEARCH
Biyoaktif Cam ve Nano-Çinko Silisyum Kompleksi İçeren Kompozit Rezinlerin Candida Albicans Adezyonu Açısından Karşılaştırılması
Comparison of Composite Resins Containing Bioactive Glass and Nano-Zinc Silicium Complex in Terms of Candida Albicans Adhesion
Makbule Tuğba TUNÇDEMİRa, Yasemin Derya GÜLSERENb
aKonya Necmettin Erbakan Üniversitesi Diş Hekimliği Fakültesi, Restoratif Diş Tedavisi ABD, Konya, TÜRKİYE
bKonya Necmettin Erbakan Üniversitesi Meram Tıp Fakültesi, Tıbbi Mikrobiyoloji ABD, Konya, TÜRKİYE
Turkiye Klinikleri J Dental Sci. 2020;26(1):67-74
doi: 10.5336/dentalsci.2019-65108
Article Language: TR
Full Text
ÖZET
 Amaç: İki farklı antibakteriyel ajan ile üretilmiş kompozitlerin Candida albicans (C. albicans) adezyonunu değerlendirmektir. Gereç ve Yöntemler: Biyoaktif Cam (BAC) ve Nano-Çinko Silisyum (NÇS) içeren kompozitlerden toplamda 40 adet örnek hazırlandı (n=10). Deney grupları 1. Grup: Kontrol (antibakteriyel ajan ilavesi yok); 2. Grup: %10 BAC; 3. Grup: %10 NÇS; 4. Grup: %10 BAC +%10 NÇS olarak oluşturuldu. Her grupta örneklerin yarısına tükürükle muamele edildi, diğer yarısı distile suda bekletildi. C. albicans ATCC 14053 Sabouraud Dextrose Agar'da 37°C'de aerobik koşullarda 48 saat inkübe edildi. Örneklerin üzerine 2' şer mililitre hazırlanan maya solüsyonu dağıltıldı ve 37°C'de başlangıç adezyonu için 2 saat inkübe edildi. Mikroskobik inceleme için örnekler steril metilen mavisiyle boyandı. Bağlanması gerçekleşen maya hücreleri ışık mikroskobu altında bir araştırmacı tarafından sayıldı. Sonuçlar hücre sayısı/ alan şeklinde belirlendi. Elde edilen veriler Two-way ANOVA testi analiz edildi. Bulgular: Materyal çeşidinin C. albicans adezyonuna etkisi olduğu tespit edildi (p=0,006). Tükürükle kaplı olmasının C. albicans adezyonunda etkili olduğu saptandı (p=0,001). Tükürükle kaplı örneklerde, BAC'nın C. albicans adezyonu NÇS ile karşılaştırıldığında istatistiksel olarak anlamlı bulundu (p=0,047). Tükürükle kaplı olmayan örneklerde, BAC'da kontrol grubuna göre C. albicans adezyonunu istatistiksel olarak anlamlı olduğu tespit edildi (p=0,02). Sonuç: Farklı antibakteriyel ajan içeren kompozit gruplarının farklı miktarlarda C. albicans adezyonu gösterdikleri tespit edildi. Tükürükle muamelenin, deney gruplarında C. Albicans adezyonunu artırdığı gözlendi.

Anahtar Kelimeler: Candida albicans; biyoaktif cam; kompozit rezin
ABSTRACT
 Objective: The aim of this study was to evaluate Candida albicans (C. albicans) adhesion of composites with two different antibacterial agents. Material and Methods: A total of 40 samples were prepared from composites containing bioactive glass (BAC) and Nano- Zinc Silicium (NÇS) (n=10). Experimental groups were fabricated as Group 1: Control (no addition of antibacterial agent); Group 2: 10% BAC; Group 3: 10% NÇS; Group 4: 10% BAC+10% NÇS. In each group half of the samples were treated with saliva and the others were kept in distilled water. C. albicans ATCC 14053 was incubated at 37C for 48 hours in aerobic conditions on Sabouraud Dextrose Agar. 2 mL of prepared yeast solution was dispersed onto the samples and incubated for 2 hours at 37C for initial adhesion. The samples were stained with sterile methylene blue. Attached yeast cells were counted by a researcher under light microscopy. The results were determined as cell number/area. Data were analyzed using two-way ANOVA test. Results: Material type had an effect on C. albicans adhesion (p=0.006). Saliva coated was effective on C. albicans adhesion (p=0.001). There was a significantly difference between BAC-NÇS in saliva-coated samples (p=0.047). C. albicans adhesion on BAC was found statistically significant than control group in non-saliva samples (p<0.05). Conclusion: Composite groups which contain different antibacterial agents showed different amounts of C. Albicans adhesion. It was observed that treatment with saliva increased C. albicans adhesion in experimental groups.

Keywords: Candida albicans; bioactive glass; composite resin
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"Turkiye Klinikleri" in its sole discretion may change the hereby "Terms of Use" anytime announcing within the "SITE". The changed terms of the hereby "Terms of Use" will become valid when they are announced. Hereby "Terms of Use" cannot be changed by unilateral declarations of users.

7. FORCE MAJEURE

"Turkiye Klinikleri" is not responsible for executing late or never of this hereby "Terms of Use", privacy policy and "USER Contract" in any situation legally taken into account as force majeure. Being late or failure of performance or non-defaulting of this and similar cases like this will not be the case from the viewpoint of "Turkiye Klinikleri", and "Turkiye Klinikleri" will not have any damage liability for these situations. "Force majeure" term will be regarded as outside of the concerned party's reasonable control and any situation that "Turkiye Klinikleri" cannot prevent even though it shows due diligence. Also, force majeure situations include but not limited to natural disasters, rebellion, war, strike, communication problems, infrastructure and internet failure, power cut and bad weather conditions.

8. LAW AND AUTHORISATION TO FOLLOW

Turkish Law will be applied in practicing, interpreting the hereby "Terms of Use" and managing the emerging legal relationships within this "Terms of Use" in case of finding element of foreignness, except for the rules of Turkish conflict of laws. Ankara Courts and Enforcement Offices are entitled in any controversy happened or may happen due to hereby contract.

9. CLOSING AND AGREEMENT

Hereby "Terms of Use" come into force when announced in the "SITE" by "Turkiye Klinikleri". The users are regarded to agree to hereby contract terms by using the "SITE". "Turkiye Klinikleri" may change the contract terms and the changes will be come into force by specifying the version number and the date of change on time it is published in the "SITE".

 

30.03.2014

Privacy Policy

We recommend you to read the terms of use below before you visit our website. In case you agree these terms, following our rules will be to your favor. Please read our Terms of Use thoroughly.

www.turkiyeklinikleri.com website belongs to Ortadoğu Advertisement Presentation Publishing Tourism Education Architecture Industry and Trade Inc. and is designed in order to inform physicians in the field of health

www.turkiyeklinikleri.com cannot reach to user’s identity, address, service providers or other information. The users may send this information to the website through forms if they would like to. However, www.turkiyeklinikleri.com may collect your hardware and software information. The information consists of your IP address, browser type, operating system, domain name, access time, and related websites. www.turkiyeklinikleri.com cannot sell the provided user information (your name, e-mail address, home and work address, phone number) to the third parties, publish it publicly, or keep it in the website. Gathered information has a directing feature to be a source for the website’s visitor profile, reporting and promotion of the services.

www.turkiyeklinikleri.com uses the taken information:

-To enhance, improve and maintain the quality of the website

-To generate visitor’s profile and statistical data

-To determine the tendency of the visitors on using our website

-To send print publications/correspondences

-To send press releases or notifications through e-mail

-To generate a list for an event or competition

By using www.turkiyeklinikleri.com you are considered to agree that;

-Ortadoğu Advertisement Presentation Publishing Tourism Education Architecture Industry and Trade Inc. cannot be hold responsible for any user’s illegal and immoral behavior,

-Terms of use may change from time to time,

-It is not responsible for other websites’ contents it cannot control or the harms they may cause although it uses the connection they provided.

Ortadoğu Advertisement Presentation Publishing Tourism Education Architecture Industry and Trade Inc. may block the website to users in the following events:

-Information with wrong, incomplete, deceiving or immoral expressions is recorded to the website,

-Proclamation, advertisement, announcement, libelous expressions are used against natural person or legal identity,

-During various attacks to the website,

-Disruption of the website because of a virus.

Written, visual and audible materials of the website, including the code and the software are under protection by legal legislation.

Without the written consent of Ortadoğu Advertisement Presentation Publishing Tourism Education Architecture Industry and Trade Inc. the information on the website cannot be downloaded, changed, reproduced, copied, republished, posted or distributed.

All rights of the software and the design of the website belong to Ortadoğu Advertisement Presentation Publishing Tourism Education Architecture Industry and Trade Inc.

Ortadoğu Advertisement Presentation Publishing Tourism Education Architecture Industry and Trade Inc. will be pleased to hear your comments about our terms of use. Please share the subjects you think may enrich our website or if there is any problem regarding our website.

info@turkiyeklinikleri.com