Turkiye Klinikleri Journal of Ophthalmology

.: ORIGINAL RESEARCH
Assessment of Corneal Endothelial Cell Status in Aging Eye
Yaşlanmanın Kornea Endotel Hücre Özellikleri Üzerine Etkisinin Değerlendirilmesi
Zeynep KAYAARASI ÖZTÜRKERa, Serpil AKARa, Serhat İMAMOĞLUb, Aslı ÖZBEK FİGUEROAc, Serpil YAZGANd, Ömer Faruk YILMAZc
aDepartment of Eye Diseases, Başkent University Faculty of Medicine,
bClinic of Eye Diseases, Haydarpaşa Numune Training and Research Hospital,
cDepartment of Eye Diseases, Yeni Yüzyıl University Faculty of Medicine, Gaziosmanpaşa Hospital, İstanbul, TURKEY
dDepartment of Eye Diseases, Zonguldak Bülent Ecevit University, Faculty of Medicine, Zonguldak, TURKEY
Turkiye Klinikleri J Ophthalmol. 2019;28(1):16-22
doi: 10.5336/ophthal.2018-60329
Article Language: EN
Full Text
ABSTRACT
 Objective: To describe the corneal endothelial cell density and morphology in normal adult eyes, and determine the influence of aging on these parameters. Material and Methods: In this cross-sectional study, specular microscopy was performed in 547 eyes of 547 healthy volunteers aged 20 to 85 years. Of these, 279 were female, and 268 were male. The study parameters included endothelial cell density (ECD), cell area (CA), the coefficient of variation (CoV) and percentage of hexagonal cells (Hex%). The influence of age and gender on these parameters were evaluated. Results: The mean ECD and mean CA in the study population were 2607±323 cells/mm2 and 389.6±51.2 μm2, respectively. In the correlation analysis, there was a decrease in mean ECD (r= - 0.305, p<0.001), and an increase in mean CA (r=0.295, p<0.001) with increasing age. The mean CoV in cell size and the mean Hex% were 35.7±6.06% and 56.8±9.1%, respectively. There was a weak correlation between increasing age and CoV in cell size (r=0.075, p=0.021), and the percentage of hexagonal was not correlated with age (p=0.088). There were no statistically significant differences in mean ECD, mean CoV in cell size, or hexagonality between genders (all p values>0.05). Conclusion: Our data for the corneal endothelial structure in Turkish population showed that age but not gender, has a negative impact on endothelial cell density. Age was not a determinant in the pleomorphic changes of the endothelium.

Keywords: Corneal endothelium; corneal endothelial cell loss; specular microscopy
ÖZET
 Amaç: Sağlıklı ve yetişkin gözlerde kornea endotel hücre yoğunluğunu ve morfolojisini tanımlamak ve yaşlanmanın bu parametreler üzerindeki etkisini incelemek. Gereç ve Yöntemler: Bu kesitsel çalışmada, 20-85 yaş arasındaki 547 sağlıklı olgunun 547 gözü çalışmaya dahil edildi. Olguların 279'u kadın, 268'i erkek idi. Speküler mikroskopi ile santral korneada yapılan analizlerde endotel hücre yoğunluğu (EHY), endotel hücre alanı (HA), endotel hücre varyasyon katsayısı (VK) ve altıgen hücrelerin yüzdesi (%Hex) değerlendirildi. Yaş ve cinsiyetin bu parametreler üzerindeki etkisi araştırıldı. Bulgular: Çalışma grubundaki ortalama EHY ve ortalama endotel HA, sırasıyla 2607±323 hücre/mm2 ve 389,6±51,2 μm2 idi. Korelasyon analizinde yaşın artması ile ortalama EHY'da (r=-0,305, p<0,001) ve ortalama endotel HA'nda (r=0,295, p<0,001) azalma tespit edildi. Endotel hücre varyasyon katsayısı ve altıgen hücre yüzdesi sırasıyla %35,7±6,06 ve %56,8±9,1 idi. Yaşın ilerlemesi ile endotel hücre VK arasında zayıf korelasyon bulundu (r=0,075, p=0,021). Altıgen hücrelerin yüzdesi (%Hex) yaş ile korele bulunmadı (p=0,088). Her iki cinsiyet arasında ortalama EHY, endotel hücre varyasyon katsayısı ve altıgen hücre yüzdesi açısından anlamlı fark bulunmadı (tüm parametreler için p>0,05). Sonuç: Türk popülasyonundaki kornea endotel hücre sayısı, yaşlanma ile olumsuz yönde etkilenmekle birlikte, pleomorfik değişikliklerin yaşla ilgisi bulunmamıştır. Her iki cins arasında ortalama endotel hücre yoğunluğu ve hücre morfolojisi açısından tüm yaş gruplarında bir fark saptanmamıştır.

Anahtar Kelimeler: Kornea endoteli; kornea endotel hücre kaybı; speküler mikroskopi
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