Turkiye Klinikleri Journal of Ophthalmology

.: ORIGINAL RESEARCH
Ambliyopi Modelinde, Rolipramin Vizüel Kortikal Plastisite Üzerine Etkisi: Deneysel Hayvan Çalışması
The Effect of Rolipram on Visual Cortical Plasticity in Amblyopia Model: Experimental Animal Study
Esra EMREa , Büşra KATREb , Özgül ALTINTAŞc, Züleyha ŞIK SARMANd , Hüsnü EFENDİe , Başak ÖZKANf , Dilek AKAKINg , Özlem Tuğçe ÇİLİNGİRg , İlkay ÖZDEMİRh , Seyhun SOLAKOĞLUh
aClinic of Ophthalmology, Fatih State Hospital, Trabzon, Türkiye
bClinic of Ophthalmology, Kocaeli State Hospital, Kocaeli, Türkiye
cDepartment of Ophthalmology, Acıbadem Ali Aydınlar University, İstanbul, Türkiye
dClinic of Ophthalmology, Büyükçekmece Mimar Sinan State Hospital, İstanbul, Türkiye
eDepartment of Neurology, Kocaeli University Faculty of Medicine, Kocaeli, Türkiye
fDepartment of Ophthalmology, University of Health Sciences Süreyyapaşa Chest Disease and Thoracic Surgery Training and Research Hospital, İstanbul, Türkiye
gDeparment of Histology and Embryology, Marmara University Faculty of Medicine, İstanbul, Türkiye
hDeparment of Histology and Embryology, İstanbul University Faculty of Medicine İstanbul, İstanbul, Turkey.
Turkiye Klinikleri J Ophthalmol. 2023;32(3):144-52
doi: 10.5336/ophthal.2022-93575
Article Language: TR
Full Text
ÖZET
 Amaç: Ratlarda, deneysel monooküler deprivasyon ambliyopisi modelinde rolipramın, vizüel kortikal plastisite üzerindeki etkisinin elektrofizyolojik ve elektron mikroskobik olarak incelenmesidir. Gereç ve Yöntemler: Yirmi dört Wistar albino cinsi rat, her grupta 4 rat olacak şekilde rastgele 6 gruba ayrıldı. Kontrol grubu dışındaki ratlar dışında tüm ratların sağ göz kapakları 21-45. günleri arası sütüre edilerek monooküler deprivasyon modeli oluşturuldu. Kırk altıncı günde tüm ratlara 10 gün boyunca rolipram veya rolipramı çözmek için kullandığımız sıvı uygulandı. Sinaptik kavşaktaki ultrastrüktürel modifikasyonlar ve görsel uyarılmış potansiyellerdeki (VEP) değişiklikler analiz edildi. Bulgular: Deprivasyon ambliyopisi modelinde VEP?te P2 latansının uzadığı, amplütüdün azaldığı ve rolipram ile tedavi sonrası ise latansın kısaldığı ve amplitüdün arttığı ve kontrol grubuna yaklaştığı görüldü. Deprivasyon ambliyopisi modelinde piramidal nöronlardaki sinapsların ultrastrüktüral yapısının bozulduğu, sinaptik yarığın genişlediği, sinaptik aktif zonun uzunluğunun kısaldığı, postsinaptik dansite kalınlığının azaldığı ve rolipram ile tedavi sonrası ise bu değerlerin kontrol grubuna yaklaştığı görüldü. Sonuç: Bu çalışma, rolipramın ratlardaki ambliyopi modelinde, vizüel kortekste yapısal ve fonksiyonel iyileşmeyi sağladığını göstermektedir. Rolipram, ambliyopi tedavisinde umut vadetmektedir ve ileri araştırmalara gerek vardır.

Anahtar Kelimeler: Ambliyopi; fosfodiesteraz inhibitör; nöral plastisite; uyandırılmış potansiyeller, görsel
ABSTRACT
 Objective: Electrophysiological and electron microscopic investigation of the effect of rolipram on visual cortical plasticity in a monocular deprived rat model. Material and Methods: Twenty-four female Wistar albino rats were randomly assigned into 6 groups, 4 rats in each. The right eyelids of all rats except the control group were sutured to induce amblyopia. We subjected rats at postnatal day 21 to a monocular deprivation (MD) model until postnatal day 45. Then, the deprived eyes of the MD model rats were reopened? cümlesi çıkarılarak ?On day 46, the deprived eyes of the rats were opened.On day 46, the deprived eyes of the rats were opened. Rats were treated with rolipram or vehicle for 10 days. The ultrastructural modifications in the synaptic junction and the changes to the visual evoked potentials (VEP) were analyzed. Results: In flash VEP, the P2 latency was prolonged and the P2 amplitude was reduced in monocularly deprived rats. With rolipram treatment, the P2 latency became shorter and the amplitude increased. In the monocularly deprived rats, the ultrastructural analysis showed that the synapses in the pyramidal neurons were impaired, the synaptic clefts were expanded, the synaptic active zones were shorter, and the thicknesses of the postsynaptic density were decreased. However, rolipram treatment improved all of the structural indices in the MD rats. Conclusion: This study shows that rolipram provides structural and functional improvement in visual cortex in amblyopia model in rats. Rolipram shows promise in the treatment of amblyopia and more research is needed.

Keywords: Amblyopia; phosphodiesterase inhibitor; neuronal plasticity; evoked potentials, visual
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