Turkiye Klinikleri Journal of Dental Sciences

.: ORIGINAL RESEARCH
Alternatif Bir Endodontik Şelatör Olarak Fitik Asidin Farklı pH Değerlerindeki Biyouyumluluğunun Değerlendirilmesi: Bir in vitro Çalışma
Evaluation of the Biocompatibility at Different pH Values of Phytic Acid as an Alternative Endodontic Chelator: An in vitro Study
Hilal ERDOĞANa , Funda KONT ÇOBANKARAb
aNevşehir Hacı Bektaş Veli Üniversitesi Diş Hekimliği Fakültesi, Endodonti AD, Nevşehir, Türkiye
bSelçuk Üniversitesi Diş Hekimliği Fakültesi, Endodonti AD, Konya, Türkiye
Turkiye Klinikleri J Dental Sci. 2024;30(3):430-7
doi: 10.5336/dentalsci.2024-102001
Article Language: TR
Full Text
ÖZET
 Amaç: Kök kanal tedavisinde etilen diamin tetraasetik asit (EDTA), şelasyon amacıyla ve rejeneratif tedavilerde yaygın olarak kullanılmaktadır. Doğal bir solüsyon olan fitik asit (FA) ise EDTA'ya alternatif olma potansiyeli bulunan bir şelatördür. Bu in vitro araştırmanın amacı, farklı pH değerlerindeki FA'nın insan fibroblast hücrelerinin canlılığı üzerine etkisini değerlendirmek ve EDTA ile karşılaştırmaktır. Gereç ve Yöntemler: Araştırmada %17 EDTA-pH: 12 (orijinal), %1 FA-pH: 2 (orijinal), %1 FA-pH: 7 klinik koşulları yansıtmak amacıyla MRC5 insan fibroblast hücrelerine 10 dk boyunca uygulandı. Kontrol grubu olarak kültür medyumu kullanılan sitotoksisite değerlendirmesi için XTT testi kullanıldı. Hücre canlılıklarının % olarak elde edildiği verilerin istatistiksel değerlendirmeleri için ''KruskalWallis'' ve ''Bonferroni düzeltmeli Dunn'' testleri kullanıldı (p<0,05). Bulgular: Orijinal pH'deki EDTA (pH: 12) ve FA (pH: 2) solüsyonları için hücre canlılıklarının kontrol grubuna göre daha düşük olduğu görüldü (p<0,05). FA için pH: 2 ve pH: 7'de benzer sitotoksik etkiler izlendi (p>0,05). FA, pH: 7'de kontrol grubu ile benzer hücre canlılığı gösterdi. Tüm solüsyonlarda hücre canlılık oranının %50 ve üzerinde olduğu izlendi. Sonuç: Mevcut araştırma koşulları altında; irrigasyon solüsyonları pH'nin sitotoksik etkide belirleyici bir faktör olarak göz önüne alınması gerektiği ve FA için nötr pH'nin hücre canlılığını olumlu şekilde etkilediği söylenebilir. Ayrıca özellikle FA-pH: 7; biyouyumluluk açısından, EDTA'ya alternatif olabilecek özellikte doğal bir şelasyon ajanı olarak önerilebilir.

Anahtar Kelimeler: Fitik asit; etilen diamin tetraasetik asit; biyouyumluluk; sitotoksisite; şelasyon ajanı
ABSTRACT
 Objective: Ethylene diamine tetraacetic acid (EDTA) is widely used in root canal treatment for chelation and regenerative therapies. Phytic acid (FA), a natural solution, is a potential alternative chelator to EDTA. The aims of this in vitro study were to evaluate the effect of FA at different pH values on the viability of human fibroblast cells and to compare it with EDTA. Material and Methods: In the study, 17% EDTA-pH: 12 (original), 1% FA-pH: 2 (original), and 1% FA-pH: 7 were applied to MRC5 human fibroblast cells for 10 minutes to reflect clinical conditions. Cytotoxicity was evaluated using the XTT assay. Culture medium was used as a control. Cell viability was expressed as a percentage and statistical analysis was performed using Kruskal-Wallis and Dunn's tests with Bonferroni correction (p<0.05). Results: Cell viabilities for EDTA (pH: 12) and FA (pH: 2) solutions at the original pH were lower than the control group (p<0.05). Similar cytotoxic effects were observed for FA at pH: 2 and pH: 7 (p>0.05). FA showed similar cell viability as the control group at pH: 7. It was observed that the cell viability rate was 50% and above in all solutions. Conclusion: Under the current research conditions, it can be concluded that the pH of irrigation solutions should be considered as a determining factor for cytotoxic effect and neutral pH for FA positively affects cell viability. Also, especially FA-pH: 7 can be recommended as a natural chelating agent that could be an alternative to EDTA in terms of biocompatibility.

Keywords: Phytic acid; ethylene diamine tetraacetic acid; biocompatibility; cytotoxicity; chelating agent
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