Turkiye Klinikleri Journal of Medical Sciences

.: ORIGINAL RESEARCH
The Effects of Resveratrol and Pioglitazone Treatment on Serum Resistin and Apelin Levels and Histochemical Parameters in the Metabolic Syndrome Rat Model: Animal Experiment
Rat Metabolik Sendrom Modelinde Resveratrol ve Pioglitazon Tedavisinin Serum Resistin ve Apelin Düzeyleri ile Histokimyasal Parametreler Üzerine Etkileri: Hayvan Deneyi
Onur GÜRSUa, Sevgi ALTAŞb, Emir DÖNDERc, Mehmet Ferit GÜRSUb, Muhammed Emre KARAMANd
aClinic of Anesthesiology and Reanimation, Ümraniye Training and Research Hospital, İstanbul, Türkiye
bDepartment of Medical Biochemistry, Fırat University Faculty of Medicine, Elazığ, Türkiye
cDepartment of Internal Medicine, Fırat University Faculty of Medicine, Elazığ, Türkiye
dDepartment of Coach Training, Fırat University Faculty of Sport Sciences, Elazığ, Türkiye
Turkiye Klinikleri J Med Sci. 2023;43(1):127-34
doi: 10.5336/medsci.2022-92225
Article Language: EN
Full Text
ABSTRACT
 Objecvtive: The current research was aimed to determine the serum resistin and apelin levels in rats with metabolic syndrome and to examine the impact of resveratrol and pioglitazone intervention on serum resistin, apelin levels and metabolic parameters. Material and Methods: Fourty male Wistar albino rats were divided into 4 groups. Group 1 [n=10, control group; without (metabolic syndrome 'MetS')]; Group 2 (n=10, MetS group without treatment); Group 3 (n=10, MetS and treatment with resveratrol); Group 4 (n=10, MetS and treatment with pioglitazone). The rats were decapitated and resistin, apelin and biochemical parameters were studied in blood samples. Results: Apelin levels did not decreased in MetS+pioglitazone treatment group. Resistin level significantly decreased in treatment groups. Myxoid degeneration and edema in aortic vessel wall were less severe in MetS+resveratrol group. Periportal sinusoidal enlargement, congestion and lymphocyte infiltration in rat liver tissue with metabolic syndrome were absent in the group treated with resveratrol, and regressed in the group treated with pioglitazone treatment. Conclusion: Resistin and apelin concentrations have found to be increased in MetS and associated with insulin re- sistance. Although pioglitazone and resveratrol treat- ment decreased resistin levels, it was ineffective on apelin levels. Although pioglitazone treatment pro- vided improvement in histopathological changes, resveratrol treatment had more benefits on histopathological changes. In the treatment of metabolic syndrome, resveratrol may be useful in support therapy. This study is the first study which shows that pioglitazone does not effects the apelin levels in metabolic syndrome treatment

Keywords: Metabolic syndrome; resistin; apelin; resveratrol; pioglitazone
ÖZET
 Amaç: Sunulan araştırmada, metabolik sendrom oluşturulan ratların serum rezistin ve apelin seviyelerinin incelenmesi ve resveratrol ve pioglitazon tedavisinin serum resistin, apelin düzeyleri ve metabolik parametreleri nasıl etkilediğinin araştırılması amaçlandı. Gereç ve Yöntemler: Kırk erkek Wistar albino rat 4 gruba ayrıldı. Grup 1 [n=10, kontrol grubu; (metabolik sendrom 'MetS') olmadan]; Grup 2 (n=10, tedavisiz MetS grubu); Grup 3 (n=10, MetS ve resveratrol ile tedavi); Grup 4 (n=10, MetS ve pioglitazon tedavisi). Ratlar dekapite edilerek, kan örneklerinde rezistin, apelin ve biyokimyasal parametreler çalışıldı. Bulgular: MetS+pioglitazon tedavi grubunda apelin seviyeleri düşmedi. Rezistin düzeyi tedavi gruplarında önemli ölçüde azaldı. Aort damar duvarında miksoid dejenerasyon ve ödem MetS+resveratrol grubunda daha az şiddetliydi. MetS'li sıçan karaciğer dokusunda periportal sinüzoidal genişleme, tıkanıklık ve lenfosit infiltrasyonu resveratrol ile tedavi edilen grupta yokken, pioglitazon tedavisi alan grupta geriledi. Sonuç: Resistin ve apelin konsantrasyonları MetS'de artmış ve insülin direnci ile ilişkili olduğu bulunmuştur. Pioglitazon ve resveratrol tedavisinin resistin düzeylerini düşürmesine karşın, apelin düzeyleri üzerine etkisisiz olduğu gösterilmiştir. Pioglitazon tedavisi histopatolojik değişikliklerde düzelme sağlasa da, resveratrol tedavisi histopatolojik değişiklikler üzerinde daha fazla fayda sağladığı gösterilmiştir. MetS tedavisinde resveratrolün destek tedavisinde faydalı olabiliceği düşünülmektedir. Bu çalışma, MetS tedavisinde pioglitazonun apelin düzeylerini etkilemediğini gösteren ilk çalışmadır.

Anahtar Kelimeler: Metabolik sendrom; resistin; apelin; resveratrol; pioglitazon
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